Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LVJ88_RS08180 Genome accession   NZ_CP091509
Coordinates   1780406..1781533 (+) Length   375 a.a.
NCBI ID   WP_054599157.1    Uniprot ID   -
Organism   Neisseria dumasiana strain LMG 30012     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1775406..1786533
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVJ88_RS08160 (LVJ88_08160) - 1775571..1776959 (-) 1389 WP_054599081.1 nucleobase:cation symporter-2 family protein -
  LVJ88_RS08165 (LVJ88_08165) dapA 1777249..1778124 (+) 876 WP_085417663.1 4-hydroxy-tetrahydrodipicolinate synthase -
  LVJ88_RS08170 (LVJ88_08170) bamC 1778141..1779274 (+) 1134 WP_085417664.1 outer membrane protein assembly factor BamC -
  LVJ88_RS08175 (LVJ88_08175) - 1779549..1780253 (-) 705 WP_054599156.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  LVJ88_RS08180 (LVJ88_08180) pilU 1780406..1781533 (+) 1128 WP_054599157.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LVJ88_RS08185 (LVJ88_08185) - 1781713..1783188 (+) 1476 WP_158087925.1 hypothetical protein -
  LVJ88_RS08190 (LVJ88_08190) - 1783279..1783821 (-) 543 WP_085417666.1 carboxymuconolactone decarboxylase family protein -
  LVJ88_RS08195 (LVJ88_08195) - 1784108..1784956 (-) 849 WP_085417667.1 DNA adenine methylase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 42153.31 Da        Isoelectric Point: 6.3459

>NTDB_id=651828 LVJ88_RS08180 WP_054599157.1 1780406..1781533(+) (pilU) [Neisseria dumasiana strain LMG 30012]
MVENPLPAKEEIYSLLKTLNKYKGSDLFITANFPPAMKLDGKITRINDTPLTPERCMEIAFSIMAPKQMDEFMNTNECNF
AISLPDTSRFRVNAMVQRGATALVFRTITSNIPKFETLNLPPVLKQIAMQKRGLVIFVGGTGSGKSTSLASMIDYRNENS
HDHIITIEDPIEFVHSHKNCIITQREVGVDTENWFAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHAN
STNQALDRIINFFPEERRTQLLTDLSLNLQAFISQRLIPREGGKGRVAAVEVLLNSPLIAELIHNGDVHSIKEIMKKSQS
QGMQTFDQALYELYENGHISFQDAIKNADSAHDLRLDIQLRSRRAQNAGPDLELI

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=651828 LVJ88_RS08180 WP_054599157.1 1780406..1781533(+) (pilU) [Neisseria dumasiana strain LMG 30012]
ATGGTTGAAAATCCTCTGCCCGCTAAAGAAGAAATCTACTCTTTATTGAAAACATTGAATAAATACAAAGGTTCCGATCT
CTTTATCACAGCCAATTTCCCGCCGGCCATGAAGCTCGACGGCAAAATTACCCGCATCAACGACACCCCGCTCACTCCCG
AGCGGTGCATGGAAATCGCCTTTTCCATTATGGCCCCCAAACAGATGGATGAGTTCATGAATACCAACGAATGCAACTTC
GCCATCAGCCTGCCCGATACCAGCCGCTTCCGTGTAAATGCAATGGTTCAACGCGGTGCAACGGCATTGGTGTTCCGTAC
CATTACCAGCAACATTCCCAAATTTGAAACCCTCAATCTGCCGCCTGTGCTGAAACAGATCGCCATGCAAAAGCGCGGTT
TGGTGATTTTTGTCGGCGGCACCGGTTCGGGCAAGTCCACCTCTTTGGCTTCCATGATCGACTACCGCAACGAAAACAGC
CACGATCATATCATCACCATCGAAGACCCGATTGAGTTTGTACACAGCCATAAAAACTGCATCATTACCCAGCGCGAGGT
CGGCGTGGATACCGAAAACTGGTTTGCTGCTTTGAAAAACACATTGCGCCAAGCCCCCGATGTTATTTTAATCGGTGAGA
TCCGCGATCGCGAAACCATGGATTACGCCATTGCCTTCGCCGAAACCGGCCACTTATGTATGGCTACCCTTCACGCCAAC
AGCACCAACCAAGCGCTCGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGCACCCAGCTGCTGACCGACCTATCGCT
CAACCTGCAAGCCTTTATCTCGCAACGCCTCATCCCGCGCGAAGGCGGCAAAGGCCGTGTGGCTGCCGTAGAAGTGCTGC
TCAATTCGCCGCTGATTGCCGAACTGATCCACAACGGCGATGTGCATTCGATTAAAGAAATCATGAAAAAATCACAAAGC
CAAGGCATGCAAACCTTCGACCAAGCTTTATACGAATTATACGAAAACGGCCACATCAGCTTTCAAGATGCCATTAAAAA
TGCCGACTCCGCCCACGACCTGCGCTTGGATATCCAATTGCGCAGCCGCCGCGCTCAAAACGCGGGGCCGGATTTGGAGC
TGATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-257)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.511

93.867

0.568
  pilU Acinetobacter baylyi ADP1

57.925

92.533

0.536
  pilU Vibrio cholerae strain A1552

53.203

95.733

0.509
  pilT Legionella pneumophila strain Lp02

44.012

89.067

0.392
  pilT Legionella pneumophila strain ERS1305867

44.012

89.067

0.392
  pilT Acinetobacter nosocomialis M2

43.713

89.067

0.389
  pilT Acinetobacter baumannii D1279779

43.713

89.067

0.389
  pilT Acinetobacter baumannii strain A118

43.713

89.067

0.389
  pilT Acinetobacter baylyi ADP1

42.647

90.667

0.387
  pilT Pseudomonas aeruginosa PAK

42.899

90.133

0.387
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.604

90.133

0.384
  pilT Pseudomonas stutzeri DSM 10701

42.012

90.133

0.379
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.042

88.8

0.373
  pilT Vibrio cholerae strain A1552

42.042

88.8

0.373
  pilT Neisseria meningitidis 8013

40.29

92

0.371
  pilT Neisseria gonorrhoeae MS11

39.826

91.733

0.365