Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L3070_RS18725 Genome accession   NZ_CP091492
Coordinates   3919091..3920071 (-) Length   326 a.a.
NCBI ID   WP_094935388.1    Uniprot ID   -
Organism   Enterobacter cloacae complex sp. ECL405     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3914091..3925071
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L3070_RS18695 (L3070_18695) - 3914967..3915464 (+) 498 WP_094935386.1 SprT family zinc-dependent metalloprotease -
  L3070_RS18700 (L3070_18700) endA 3915559..3916266 (+) 708 WP_023333343.1 deoxyribonuclease I -
  L3070_RS18705 (L3070_18705) rsmE 3916318..3917049 (+) 732 WP_023309126.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  L3070_RS18710 (L3070_18710) gshB 3917069..3918016 (+) 948 WP_094935387.1 glutathione synthase -
  L3070_RS18715 (L3070_18715) - 3918104..3918664 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  L3070_RS18720 (L3070_18720) ruvX 3918664..3919080 (+) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  L3070_RS18725 (L3070_18725) pilT 3919091..3920071 (-) 981 WP_094935388.1 type IV pilus twitching motility protein PilT Machinery gene
  L3070_RS18730 (L3070_18730) - 3920089..3920790 (+) 702 WP_094935389.1 YggS family pyridoxal phosphate-dependent enzyme -
  L3070_RS18735 (L3070_18735) - 3920812..3921378 (+) 567 WP_087823219.1 YggT family protein -
  L3070_RS18740 (L3070_18740) yggU 3921375..3921671 (+) 297 WP_023309131.1 DUF167 family protein YggU -
  L3070_RS18745 (L3070_18745) - 3921675..3922268 (+) 594 WP_033146609.1 XTP/dITP diphosphatase -
  L3070_RS18750 (L3070_18750) hemW 3922261..3923403 (+) 1143 WP_094935390.1 radical SAM family heme chaperone HemW -
  L3070_RS18755 (L3070_18755) - 3923467..3923829 (+) 363 WP_094935391.1 endonuclease domain-containing protein -
  L3070_RS18760 (L3070_18760) - 3923891..3924607 (-) 717 WP_024906551.1 DUF2884 domain-containing protein -
  L3070_RS18765 (L3070_18765) - 3924664..3924990 (-) 327 WP_094935392.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35523.55 Da        Isoelectric Point: 6.9108

>NTDB_id=651703 L3070_RS18725 WP_094935388.1 3919091..3920071(-) (pilT) [Enterobacter cloacae complex sp. ECL405]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFQPPDVGALLKAWLNDEQQGTWWAKGQVDFAATVTGGQRLRG
SAFKQMRGVSVTLRLLPRSCPQLASLGAPRVIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHADGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFAEALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRSQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=651703 L3070_RS18725 WP_094935388.1 3919091..3920071(-) (pilT) [Enterobacter cloacae complex sp. ECL405]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGATTAGGCCGGCTTGAACCCGCTCCCTTTCAGCCTCCCGACGTTGGGGCGTTATTAAAAGCGTGGCTTAACG
ATGAGCAGCAGGGAACATGGTGGGCAAAGGGCCAGGTGGATTTTGCCGCGACGGTAACGGGAGGCCAGCGCCTGCGCGGC
AGTGCCTTTAAGCAGATGAGAGGTGTTTCTGTGACGCTGCGGCTGTTGCCGCGTAGCTGCCCGCAGCTCGCTTCGCTGGG
CGCGCCGCGGGTAATCCCGGAACTGTTATCCAATGACGCCGGGCTGATTCTGGTCACGGGGGCGACCGGCAGCGGCAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTAAACCACCATGCTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCTGAAGCGCTGCG
CAGCGCCTTACGCCAGGATCCGGACGTTATTCTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAAACCGGACATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAGCCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAGAGGCTGCTTCC
CGATCTGCAGGGCGGGCGCGTCGCGTTGTATGAACTGCTGGTGAACACAGCGGCGGCGGCGAATCTGATTCGTGAAGGGA
AAACGTGGCAACTGCCCGGCATCATTCAAACCGGTCAGCAGGCAGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(88-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Pseudomonas stutzeri DSM 10701

48.93

100

0.491
  pilT Pseudomonas aeruginosa PAK

48.318

100

0.485
  pilT Acinetobacter baumannii D1279779

48.318

100

0.485
  pilT Acinetobacter baumannii strain A118

48.318

100

0.485
  pilT Legionella pneumophila strain ERS1305867

49.068

98.773

0.485
  pilT Legionella pneumophila strain Lp02

49.068

98.773

0.485
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Acinetobacter baylyi ADP1

48.012

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter nosocomialis M2

48.75

98.16

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.789

98.773

0.433
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.402
  pilU Vibrio cholerae strain A1552

39.752

98.773

0.393
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362