Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA/pilA1   Type   Machinery gene
Locus tag   LZ558_RS04480 Genome accession   NZ_CP091424
Coordinates   993286..993705 (-) Length   139 a.a.
NCBI ID   WP_326498444.1    Uniprot ID   -
Organism   Methylobacter sp. YRD-M1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 988286..998705
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LZ558_RS04465 (LZ558_04440) mutS 988381..990948 (-) 2568 WP_268119636.1 DNA mismatch repair protein MutS -
  LZ558_RS04470 (LZ558_04445) - 990987..991490 (+) 504 WP_268119637.1 CinA family protein -
  LZ558_RS04475 (LZ558_04450) - 991529..993037 (-) 1509 WP_268119639.1 hypothetical protein -
  LZ558_RS04480 (LZ558_04455) pilA/pilA1 993286..993705 (-) 420 WP_326498444.1 pilin Machinery gene
  LZ558_RS04490 (LZ558_04465) - 994164..994406 (+) 243 WP_268119640.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
  LZ558_RS04495 (LZ558_04470) - 994399..994791 (+) 393 WP_268119641.1 type II toxin-antitoxin system VapC family toxin -
  LZ558_RS04500 (LZ558_04475) - 994953..995150 (+) 198 WP_268119643.1 type II toxin-antitoxin system VapB family antitoxin -
  LZ558_RS04505 (LZ558_04480) - 995147..995518 (+) 372 WP_268119644.1 type II toxin-antitoxin system VapC family toxin -
  LZ558_RS04510 (LZ558_04485) - 995655..996176 (-) 522 WP_268119645.1 DUF1264 domain-containing protein -
  LZ558_RS04515 (LZ558_04490) - 996954..997367 (+) 414 WP_268119646.1 hypothetical protein -
  LZ558_RS04520 (LZ558_04495) - 997401..998000 (-) 600 WP_268119647.1 hypothetical protein -
  LZ558_RS04525 (LZ558_04500) - 998121..998312 (+) 192 WP_268119648.1 hypothetical protein -

Sequence


Protein


Download         Length: 139 a.a.        Molecular weight: 14264.41 Da        Isoelectric Point: 8.4477

>NTDB_id=651089 LZ558_RS04480 WP_326498444.1 993286..993705(-) (pilA/pilA1) [Methylobacter sp. YRD-M1]
MKTQMRKVQQGFTLIELMIVVAIIGILAAIAIPAYNDYTAKAQASEAFTLIDGLKTPLSANLSETGAWAIPAGSVTAGKY
VASIVSGGAAGAYTLLATYVANGANSQIVGKTVTFTYNDATGAWSCASNLPNNIKPASC

Nucleotide


Download         Length: 420 bp        

>NTDB_id=651089 LZ558_RS04480 WP_326498444.1 993286..993705(-) (pilA/pilA1) [Methylobacter sp. YRD-M1]
ATGAAAACTCAAATGAGAAAAGTACAACAAGGTTTTACCTTAATCGAATTAATGATCGTCGTTGCGATCATCGGTATTTT
GGCGGCGATTGCGATTCCGGCTTATAACGACTACACTGCAAAAGCTCAGGCTTCTGAAGCTTTCACTTTAATTGATGGTC
TTAAAACTCCGCTTTCTGCAAATCTCTCAGAGACTGGCGCTTGGGCTATTCCGGCTGGCTCTGTAACTGCTGGTAAATAT
GTTGCTTCGATTGTATCTGGCGGCGCTGCTGGTGCTTATACACTTCTGGCAACCTATGTCGCTAACGGAGCAAATAGTCA
AATTGTCGGCAAAACAGTGACTTTTACTTACAATGATGCAACCGGTGCATGGTCTTGTGCATCTAATCTACCTAATAATA
TTAAACCTGCTTCTTGCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Eikenella corrodens VA1

44.516

100

0.496

  pilA2 Legionella pneumophila str. Paris

44.755

100

0.46

  comP Acinetobacter baylyi ADP1

43.448

100

0.453

  pilA2 Legionella pneumophila strain ERS1305867

42.657

100

0.439

  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.411

100

0.424

  pilA Vibrio parahaemolyticus RIMD 2210633

42.963

97.122

0.417

  pilA Acinetobacter nosocomialis M2

54.286

75.54

0.41

  pilA Pseudomonas aeruginosa PAK

37.584

100

0.403

  pilA Acinetobacter baumannii strain A118

40

100

0.403

  pilE Neisseria gonorrhoeae MS11

40.741

97.122

0.396

  pilE Neisseria gonorrhoeae strain FA1090

41.221

94.245

0.388

  pilA Vibrio cholerae C6706

33.54

100

0.388

  pilA Vibrio cholerae strain A1552

33.54

100

0.388

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

33.54

100

0.388

  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.486

100

0.388

  pilA Glaesserella parasuis strain SC1401

37.5

97.842

0.367