Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OHB13_RS34495 Genome accession   NZ_CP107921
Coordinates   7751264..7752376 (+) Length   370 a.a.
NCBI ID   WP_323183960.1    Uniprot ID   -
Organism   Streptomyces sp. NBC_00440     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7746264..7757376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OHB13_RS34480 (OHB13_34460) - 7746473..7746922 (+) 450 WP_328379766.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OHB13_RS34485 (OHB13_34465) - 7746919..7747443 (+) 525 WP_328379767.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OHB13_RS34490 (OHB13_34470) - 7747440..7751267 (+) 3828 WP_328379768.1 hypothetical protein -
  OHB13_RS34495 (OHB13_34475) pilT 7751264..7752376 (+) 1113 WP_323183960.1 type IV pilus twitching motility protein PilT Machinery gene
  OHB13_RS34500 (OHB13_34480) - 7752554..7753519 (-) 966 WP_328379769.1 pirin family protein -
  OHB13_RS34505 (OHB13_34485) - 7753798..7754457 (+) 660 WP_328380458.1 pyridoxal 5'-phosphate synthase -
  OHB13_RS34510 (OHB13_34490) - 7754626..7755297 (+) 672 WP_328379770.1 ABC transporter permease -
  OHB13_RS34515 (OHB13_34495) - 7755294..7755968 (+) 675 WP_328379771.1 ABC transporter permease subunit -
  OHB13_RS34520 (OHB13_34500) - 7755965..7756915 (+) 951 WP_328380459.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40056.57 Da        Isoelectric Point: 5.9636

>NTDB_id=649430 OHB13_RS34495 WP_323183960.1 7751264..7752376(+) (pilT) [Streptomyces sp. NBC_00440]
MSKHATLEATMPGPSSANIDHLLGELWERGGSDLLLTAGSAPFLRVDGALRPVEGAALTEPEVDRLVTGVLGEELTERFR
QEKQVDFAFSWGEKARLRGNAFVQRGASALALRIIPFDVPSSEQLGLPPVVIGWAGMPRGFVLVTGPTGSGKSTSLAALL
DYVNTHRSVHILTIEDPIEYLHRHKRSAVNQREVGTDTDSFPAALRSALREDPDVLLLGEMRDPESISAALTIAETGHLV
FATLHTNDTSQTLDRIVDVFPAEQQPQIRLQLAHTLVGILNQTLIPRVGGGRVAAFEVLVGTSAIRNLIREGKTRQIRNM
ISTGHREGMQTLETSINALVAAGTIAYEDAVSYTACPEDIQRPHVPVGRI

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=649430 OHB13_RS34495 WP_323183960.1 7751264..7752376(+) (pilT) [Streptomyces sp. NBC_00440]
ATGAGCAAACACGCCACCTTGGAGGCCACCATGCCCGGGCCGAGTTCCGCGAACATTGACCACCTGCTCGGTGAGCTGTG
GGAGCGGGGCGGCAGCGACCTGCTGCTCACCGCCGGATCGGCGCCGTTCCTCCGCGTCGACGGCGCCCTGCGACCGGTCG
AGGGGGCCGCGCTGACCGAGCCGGAGGTCGATCGCCTGGTGACCGGGGTGCTGGGCGAGGAACTCACGGAACGGTTCCGC
CAGGAGAAGCAGGTCGATTTCGCGTTCAGCTGGGGGGAGAAGGCCAGGCTGCGCGGGAACGCTTTCGTGCAGCGCGGCGC
GTCGGCGCTGGCGCTGCGGATCATCCCGTTCGACGTGCCGTCGTCCGAGCAGCTCGGGCTCCCCCCGGTCGTCATCGGAT
GGGCGGGCATGCCGCGCGGGTTCGTGCTGGTCACCGGGCCGACCGGTTCTGGGAAGTCGACAAGCCTGGCGGCGCTCCTC
GACTACGTCAACACGCACCGGTCGGTGCACATCCTCACCATTGAGGACCCGATCGAGTACCTGCACCGGCACAAGCGCTC
GGCGGTCAACCAGCGTGAGGTCGGTACCGACACCGACTCATTCCCGGCCGCGCTGCGCTCGGCCCTGCGCGAGGATCCCG
ACGTCCTGCTCCTCGGCGAGATGCGCGACCCGGAGAGCATCTCCGCGGCCCTGACCATCGCCGAGACCGGGCACCTGGTG
TTCGCGACCCTGCACACCAACGACACGTCGCAGACGCTGGACCGCATCGTCGACGTGTTCCCGGCCGAGCAACAGCCGCA
GATCCGGCTGCAATTGGCGCATACCCTGGTCGGCATCCTCAACCAGACGCTCATCCCCCGGGTCGGCGGGGGCCGGGTGG
CCGCCTTCGAGGTGCTGGTAGGCACGTCGGCGATCCGCAACCTGATCCGCGAGGGCAAGACTCGCCAGATCCGCAACATG
ATCTCGACCGGCCACCGTGAAGGCATGCAGACTCTTGAGACCAGCATCAACGCCCTCGTCGCGGCAGGCACCATCGCCTA
CGAGGACGCCGTGTCGTACACGGCCTGCCCGGAGGACATCCAGCGGCCCCACGTCCCGGTCGGTCGCATCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(138-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

50.581

92.973

0.47
  pilT Pseudomonas stutzeri DSM 10701

49.709

92.973

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.138

94.324

0.454
  pilT Acinetobacter baumannii D1279779

48.974

92.162

0.451
  pilT Acinetobacter baumannii strain A118

48.974

92.162

0.451
  pilT Acinetobacter nosocomialis M2

48.68

92.162

0.449
  pilT Acinetobacter baylyi ADP1

48.387

92.162

0.446
  pilT Neisseria meningitidis 8013

49.102

90.27

0.443
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.512

90.811

0.441
  pilT Vibrio cholerae strain A1552

48.512

90.811

0.441
  pilT Neisseria gonorrhoeae MS11

48.348

90

0.435
  pilT Legionella pneumophila strain ERS1305867

48.048

90

0.432
  pilT Legionella pneumophila strain Lp02

48.048

90

0.432
  pilU Pseudomonas stutzeri DSM 10701

42.319

93.243

0.395
  pilU Vibrio cholerae strain A1552

39.826

92.973

0.37
  pilU Acinetobacter baylyi ADP1

38.728

93.514

0.362