Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   L1776_RS17505 Genome accession   NZ_CP091174
Coordinates   3741842..3742249 (+) Length   135 a.a.
NCBI ID   WP_419586397.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain XX1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3736842..3747249
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1776_RS17485 coaE 3736976..3737584 (-) 609 WP_080940987.1 dephospho-CoA kinase -
  L1776_RS17490 pilD 3737716..3738582 (-) 867 WP_236450814.1 prepilin peptidase Machinery gene
  L1776_RS17495 pilC 3738587..3739804 (-) 1218 WP_236450815.1 type II secretion system F family protein Machinery gene
  L1776_RS17500 pilB 3739807..3741510 (-) 1704 WP_106442874.1 type IV-A pilus assembly ATPase PilB Machinery gene
  L1776_RS17505 pilA/pilAI 3741842..3742249 (+) 408 WP_419586397.1 pilin Machinery gene
  L1776_RS17510 pilA/pilAII 3742308..3742733 (+) 426 WP_236450817.1 pilin Regulator
  L1776_RS17515 - 3742758..3744149 (+) 1392 WP_236450818.1 O-antigen ligase family protein -
  L1776_RS17520 tfpZ 3744158..3744895 (+) 738 WP_236450819.1 TfpX/TfpZ family type IV pilin accessory protein -
  L1776_RS17525 cysN 3745018..3746916 (-) 1899 WP_236450820.1 sulfate adenylyltransferase subunit CysN -

Sequence

Protein


Download         Length: 135 a.a.        Molecular weight: 14064.01 Da        Isoelectric Point: 4.8277

>NTDB_id=648167 L1776_RS17505 WP_419586397.1 3741842..3742249(+) (pilA/pilAI) [Stutzerimonas stutzeri strain XX1]
MQKGFTLIELMIVVAIIGILAAIALPAYQDYTVRSNAAAALAEITPGKIGFEQAINEGKTPSLDPTEEGYIGITDSTSYC
GVTINPAANGNIECTAKGGNAGKFDGKKITLNRTAEGAWSCASDLESKYKPGKCS

Nucleotide


Download         Length: 408 bp        

>NTDB_id=648167 L1776_RS17505 WP_419586397.1 3741842..3742249(+) (pilA/pilAI) [Stutzerimonas stutzeri strain XX1]
ATGCAGAAAGGCTTTACCCTTATCGAACTGATGATCGTGGTTGCGATCATCGGCATTCTGGCCGCCATTGCGCTGCCGGC
TTATCAGGACTATACCGTACGCTCCAACGCTGCCGCTGCCTTGGCAGAAATCACCCCCGGCAAAATCGGTTTTGAGCAGG
CGATCAACGAAGGAAAAACTCCGTCGCTCGACCCAACCGAGGAAGGCTACATTGGTATAACCGACTCTACTTCTTACTGT
GGAGTGACTATTAATCCTGCTGCAAATGGCAATATCGAGTGTACTGCCAAAGGCGGCAATGCCGGCAAGTTTGATGGCAA
AAAAATCACGCTTAATCGCACCGCCGAGGGAGCGTGGAGCTGCGCTAGCGACTTGGAATCCAAGTACAAGCCAGGCAAGT
GTTCCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(33-134)

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

88.148

100

0.881
  pilA/pilAII Pseudomonas stutzeri DSM 10701

65.909

97.778

0.644
  pilA Pseudomonas aeruginosa PAK

45.89

100

0.496
  pilA/pilA1 Eikenella corrodens VA1

40.385

100

0.467
  pilA Acinetobacter baumannii strain A118

45

100

0.467
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.727

100

0.43
  pilA Vibrio cholerae strain A1552

41.727

100

0.43
  pilA Vibrio cholerae C6706

41.727

100

0.43
  comP Acinetobacter baylyi ADP1

40

100

0.43
  pilA2 Legionella pneumophila str. Paris

40.741

100

0.407
  pilA2 Legionella pneumophila strain ERS1305867

40.741

100

0.407
  pilA Vibrio parahaemolyticus RIMD 2210633

36.296

100

0.363