Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   L1776_RS17500 Genome accession   NZ_CP091174
Coordinates   3739807..3741510 (-) Length   567 a.a.
NCBI ID   WP_106442874.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain XX1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3734807..3746510
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1776_RS17470 - 3734992..3736032 (+) 1041 WP_236450813.1 NADP(H)-dependent aldo-keto reductase -
  L1776_RS17475 - 3736089..3736769 (+) 681 WP_013981946.1 energy-coupling factor ABC transporter permease -
  L1776_RS17480 yacG 3736782..3736979 (-) 198 WP_041771656.1 DNA gyrase inhibitor YacG -
  L1776_RS17485 coaE 3736976..3737584 (-) 609 WP_080940987.1 dephospho-CoA kinase -
  L1776_RS17490 pilD 3737716..3738582 (-) 867 WP_236450814.1 prepilin peptidase Machinery gene
  L1776_RS17495 pilC 3738587..3739804 (-) 1218 WP_236450815.1 type II secretion system F family protein Machinery gene
  L1776_RS17500 pilB 3739807..3741510 (-) 1704 WP_106442874.1 type IV-A pilus assembly ATPase PilB Machinery gene
  L1776_RS17505 pilA/pilAI 3741842..3742249 (+) 408 WP_419586397.1 pilin Machinery gene
  L1776_RS17510 pilA/pilAII 3742308..3742733 (+) 426 WP_236450817.1 pilin Regulator
  L1776_RS17515 - 3742758..3744149 (+) 1392 WP_236450818.1 O-antigen ligase family protein -
  L1776_RS17520 tfpZ 3744158..3744895 (+) 738 WP_236450819.1 TfpX/TfpZ family type IV pilin accessory protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62085.04 Da        Isoelectric Point: 5.4725

>NTDB_id=648166 L1776_RS17500 WP_106442874.1 3739807..3741510(-) (pilB) [Stutzerimonas stutzeri strain XX1]
MSENISLSGLARQMVLAGVLDERTAQQAQVQALRNKTSFVTYVVQNKLAKGRAVAEVASEQFGVALLDLAALDKEVPPKD
VVSEKLARQHRLLPLAKRGNKLYVAVSDPANHQAISDIQFSTGLTTEAILVEDDKLGDAIDKFFDNGSTGLEDLGDVDLD
GVDVETVSDDIKGDDGGAEADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAYRVRLRTDGILHEVARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKLSKSKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEDQKELYMNALRQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCNSCKKEVDVPRDVLLREGFP
EQKLGTFKIYGPVGCDNCKGGYKGRVGIYEVVKNTPSLQRIIMEDGNSIDISTQMRKDGFNDLRTSALLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=648166 L1776_RS17500 WP_106442874.1 3739807..3741510(-) (pilB) [Stutzerimonas stutzeri strain XX1]
ATGAGCGAAAATATTTCTCTGTCCGGGTTGGCTCGGCAGATGGTGTTGGCTGGTGTACTGGATGAGCGGACAGCACAGCA
GGCGCAGGTTCAGGCGCTGCGAAACAAAACATCCTTCGTCACCTACGTAGTCCAGAACAAATTGGCAAAGGGGCGTGCCG
TCGCTGAAGTTGCCTCGGAGCAGTTCGGCGTTGCGCTCTTGGATCTGGCAGCTCTGGACAAGGAGGTGCCGCCCAAGGAT
GTCGTCAGCGAGAAGCTGGCTCGCCAGCATCGCTTGCTCCCGCTGGCTAAGCGCGGCAATAAGCTTTACGTGGCCGTTTC
AGATCCGGCGAACCATCAGGCGATTTCCGATATTCAGTTCAGTACTGGGTTAACCACCGAGGCCATCCTCGTCGAGGACG
ACAAGCTCGGGGACGCCATCGATAAATTTTTCGATAATGGTTCAACCGGTCTGGAGGATCTGGGCGACGTGGATCTCGAC
GGAGTGGATGTCGAGACCGTTTCGGATGACATAAAAGGAGACGATGGCGGTGCGGAGGCTGACGATGCGCCGGTCGTGCG
CTTCGTCAACAAGATGCTCCTCGACGCCATTCGTGGGGGCTCATCAGACCTCCATTTCGAGCCCTATGAAAAGGCCTATC
GAGTTCGGTTGCGTACTGACGGAATTCTGCATGAGGTGGCGCGCCCGCCCATTCAACTGGCGCCTCGAATTTCGGCGCGT
CTAAAGGTCATGGCAGGGCTGGATATCTCCGAGCGGCGCAAGCCGCAGGATGGCCGCATCAAGATGAAACTCTCCAAGAG
CAAGGCCATCGATTTCCGGGTCAACACGCTACCGACCCTGTGGGGCGAAAAGATCGTGATGCGGATTCTTGATCCCTCAA
GTGCTCAAATGGGCATCGATGCGCTGGGGTATGAGGAGGATCAAAAAGAGCTGTATATGAATGCTCTGCGGCAACCGCAG
GGTATGATTCTCGTGACTGGTCCGACGGGCTCCGGTAAGACGGTATCCCTCTACACCGGGTTGAACATCCTCAACACCGT
CGATGTGAATATTTCGACGGCGGAGGATCCGGTAGAGATCAACCTCGAGGGTATCAACCAGGTCAACGTCAATCCTAAAC
AGGGCATGGACTTTTCTCAGGCCCTCCGTGCCTTTCTACGTCAGGACCCGGACATCATCATGGTAGGGGAGATCCGCGAT
CTGGAGACCGCCGAGATTGCGATCAAGGCTGCGCAGACGGGCCATATGGTGATGTCGACACTTCATACCAATAGCGCTGC
GGAAACCCTGACGCGTCTGCGCAACATGGGGGTGCCTGCGTTCAACATCGCGACCTCCGTGAACCTGATCATTGCTCAGC
GTCTGGCACGAAAGCTCTGCAATAGCTGCAAGAAAGAGGTTGATGTCCCCCGTGACGTTTTGCTGCGCGAAGGGTTCCCT
GAGCAGAAGCTTGGAACCTTCAAGATTTATGGTCCGGTAGGTTGCGACAACTGCAAGGGCGGCTACAAGGGGCGCGTTGG
TATTTATGAAGTGGTTAAAAACACGCCATCGTTGCAGCGGATTATCATGGAAGACGGAAACTCCATCGATATTTCAACCC
AGATGCGCAAAGACGGCTTCAACGACCTGCGAACCTCGGCTTTGCTGAAAGCCATGCAGGGCGTGACCAGCCTCGAAGAA
GTCAATCGGGTCACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.638

99.471

0.603
  pilB Acinetobacter baylyi ADP1

59.574

99.471

0.593
  pilB Legionella pneumophila strain ERS1305867

55.769

100

0.563
  pilB Vibrio cholerae strain A1552

51.287

95.944

0.492
  pilB Vibrio campbellii strain DS40M4

48.246

100

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

51.923

91.711

0.476
  pilF Neisseria gonorrhoeae MS11

51.731

91.711

0.474
  pilB/pilB1 Synechocystis sp. PCC 6803

35.91

100

0.393