Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L2D51_RS20290 Genome accession   NZ_CP091122
Coordinates   4247521..4248543 (-) Length   340 a.a.
NCBI ID   WP_240293712.1    Uniprot ID   -
Organism   Serratia marcescens strain CM2012_028     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4242521..4253543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2D51_RS20260 (L2D51_20260) - 4243321..4243833 (+) 513 WP_016930082.1 SprT family zinc-dependent metalloprotease -
  L2D51_RS20265 (L2D51_20265) endA 4243935..4244630 (+) 696 WP_049300546.1 deoxyribonuclease I -
  L2D51_RS20270 (L2D51_20270) rsmE 4244700..4245431 (+) 732 WP_049300547.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  L2D51_RS20275 (L2D51_20275) gshB 4245442..4246392 (+) 951 WP_240293711.1 glutathione synthase -
  L2D51_RS20280 (L2D51_20280) - 4246539..4247102 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  L2D51_RS20285 (L2D51_20285) ruvX 4247102..4247524 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  L2D51_RS20290 (L2D51_20290) pilT 4247521..4248543 (-) 1023 WP_240293712.1 type IV pilus twitching motility protein PilT Machinery gene
  L2D51_RS20295 (L2D51_20295) - 4248564..4249271 (+) 708 WP_033639392.1 YggS family pyridoxal phosphate-dependent enzyme -
  L2D51_RS20300 (L2D51_20300) proC 4249291..4250112 (+) 822 WP_016930089.1 pyrroline-5-carboxylate reductase -
  L2D51_RS20305 (L2D51_20305) - 4250144..4250698 (+) 555 WP_016930090.1 YggT family protein -
  L2D51_RS20310 (L2D51_20310) yggU 4250695..4250988 (+) 294 WP_016930091.1 DUF167 family protein YggU -
  L2D51_RS20315 (L2D51_20315) - 4251129..4251722 (+) 594 WP_016930092.1 XTP/dITP diphosphatase -
  L2D51_RS20320 (L2D51_20320) hemW 4251715..4252857 (+) 1143 WP_048796774.1 radical SAM family heme chaperone HemW -
  L2D51_RS20325 (L2D51_20325) - 4252896..4253144 (-) 249 WP_079451748.1 DUF2442 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36419.67 Da        Isoelectric Point: 7.0671

>NTDB_id=647880 L2D51_RS20290 WP_240293712.1 4247521..4248543(-) (pilT) [Serratia marcescens strain CM2012_028]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANVFQQSAGISLALRRIAGQSPSLAELAAPAIVPALLRRDDGLILVTGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGECAGEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=647880 L2D51_RS20290 WP_240293712.1 4247521..4248543(-) (pilT) [Serratia marcescens strain CM2012_028]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGGGCGGGGAG
CGGTTGCGCGCCAACGTTTTTCAACAAAGCGCGGGGATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGACGACGGGCTTATCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCATATTTTGACGCTGGAA
GATCCGATCGAGTTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACTGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCATACCCGCAGCGCGCCGCAGGCGGTAGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCCGGCGGCGGACGCGTGGCGATCTTCGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGTGTGTTGGGGGAGTGCGCGGGGGAGGGAACGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.848

97.059

0.532
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.848

97.059

0.532
  pilT Acinetobacter baylyi ADP1

51.36

97.353

0.5
  pilT Acinetobacter baumannii D1279779

50.909

97.059

0.494
  pilT Acinetobacter baumannii strain A118

50.909

97.059

0.494
  pilT Acinetobacter nosocomialis M2

50.909

97.059

0.494
  pilT Neisseria meningitidis 8013

50.301

97.647

0.491
  pilT Neisseria gonorrhoeae MS11

50.151

97.353

0.488
  pilT Legionella pneumophila strain ERS1305867

49.398

97.647

0.482
  pilT Legionella pneumophila strain Lp02

49.398

97.647

0.482
  pilT Pseudomonas aeruginosa PAK

49.697

97.059

0.482
  pilT Pseudomonas stutzeri DSM 10701

49.091

97.059

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.785

100

0.456
  pilU Acinetobacter baylyi ADP1

40.248

95

0.382
  pilU Vibrio cholerae strain A1552

39.877

95.882

0.382
  pilU Pseudomonas stutzeri DSM 10701

38.889

95.294

0.371