Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L2D50_RS20470 Genome accession   NZ_CP091120
Coordinates   4316982..4318004 (-) Length   340 a.a.
NCBI ID   WP_049204215.1    Uniprot ID   -
Organism   Serratia marcescens strain CM2019_254     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4311982..4323004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2D50_RS20440 (L2D50_20440) - 4312781..4313293 (+) 513 WP_004937462.1 SprT family zinc-dependent metalloprotease -
  L2D50_RS20445 (L2D50_20445) endA 4313395..4314090 (+) 696 WP_043141331.1 deoxyribonuclease I -
  L2D50_RS20450 (L2D50_20450) rsmE 4314160..4314891 (+) 732 WP_025304244.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  L2D50_RS20455 (L2D50_20455) gshB 4314902..4315852 (+) 951 WP_025304245.1 glutathione synthase -
  L2D50_RS20460 (L2D50_20460) - 4316000..4316563 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  L2D50_RS20465 (L2D50_20465) ruvX 4316563..4316985 (+) 423 WP_025304246.1 Holliday junction resolvase RuvX -
  L2D50_RS20470 (L2D50_20470) pilT 4316982..4318004 (-) 1023 WP_049204215.1 type IV pilus twitching motility protein PilT Machinery gene
  L2D50_RS20475 (L2D50_20475) - 4318025..4318732 (+) 708 WP_025304248.1 YggS family pyridoxal phosphate-dependent enzyme -
  L2D50_RS20480 (L2D50_20480) proC 4318752..4319573 (+) 822 WP_025304249.1 pyrroline-5-carboxylate reductase -
  L2D50_RS20485 (L2D50_20485) - 4319605..4320159 (+) 555 WP_025304250.1 YggT family protein -
  L2D50_RS20490 (L2D50_20490) yggU 4320156..4320446 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  L2D50_RS20495 (L2D50_20495) - 4320464..4321057 (+) 594 WP_043141349.1 XTP/dITP diphosphatase -
  L2D50_RS20500 (L2D50_20500) hemW 4321050..4322192 (+) 1143 WP_197791199.1 radical SAM family heme chaperone HemW -
  L2D50_RS20505 (L2D50_20505) - 4322230..4322478 (-) 249 WP_025304253.1 DUF2442 domain-containing protein -
  L2D50_RS20510 (L2D50_20510) - 4322462..4322704 (-) 243 WP_004937418.1 DUF4160 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36442.61 Da        Isoelectric Point: 7.0670

>NTDB_id=647833 L2D50_RS20470 WP_049204215.1 4316982..4318004(-) (pilT) [Serratia marcescens strain CM2019_254]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAQTLTAQGAQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANIFQQSAGISLALRRIAGQSPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTTTIRLALTAAETGHLVLATLHTRSASQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCAGEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=647833 L2D50_RS20470 WP_049204215.1 4316982..4318004(-) (pilT) [Serratia marcescens strain CM2019_254]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCAAACGCTGACGGCGCAAGGCGCGCAGGCCCTCTGCGATG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGGGCGGGGAG
CGGTTGCGCGCCAACATTTTTCAACAAAGCGCGGGGATTTCTCTCGCGTTGCGGCGTATTGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCAGCGCCGGCCATCGTGCCGGCGCTGCTGCGGCGCGACGATGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCAGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCACATTTTGACGCTGGAG
GATCCGATTGAATTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTTGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCACCACCATCCGGC
TGGCGCTCACCGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGTCGCAGGCAGTGGAACGG
TTAGTGGACGTGTTTCCGGCGGAAGAAAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGTCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGCGGCGGACGCGTGGCGATCTTCGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCACAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATTGACGCCGGTGTGTTGGGGGACTGTGCGGGGGAGGGAACGGGTGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.242

97.059

0.526
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.242

97.059

0.526
  pilT Acinetobacter baylyi ADP1

50.453

97.353

0.491
  pilT Acinetobacter baumannii D1279779

50.303

97.059

0.488
  pilT Acinetobacter baumannii strain A118

50.303

97.059

0.488
  pilT Acinetobacter nosocomialis M2

50.303

97.059

0.488
  pilT Neisseria gonorrhoeae MS11

49.254

98.529

0.485
  pilT Neisseria meningitidis 8013

49.699

97.647

0.485
  pilT Pseudomonas stutzeri DSM 10701

48.368

99.118

0.479
  pilT Legionella pneumophila strain ERS1305867

48.943

97.353

0.476
  pilT Legionella pneumophila strain Lp02

48.943

97.353

0.476
  pilT Pseudomonas aeruginosa PAK

49.091

97.059

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.38

100

0.453
  pilU Vibrio cholerae strain A1552

39.571

95.882

0.379
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.58

95.294

0.368