Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L1S38_RS03930 Genome accession   NZ_CP091081
Coordinates   793434..794414 (+) Length   326 a.a.
NCBI ID   WP_059360151.1    Uniprot ID   -
Organism   Enterobacter roggenkampii strain GD21SC1505     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 788434..799414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1S38_RS03895 (L1S38_03895) - 788806..789132 (+) 327 WP_059360146.1 YggL family protein -
  L1S38_RS03900 (L1S38_03900) - 789189..789905 (+) 717 WP_008499762.1 DUF2884 domain-containing protein -
  L1S38_RS03905 (L1S38_03905) hemW 790096..791244 (-) 1149 WP_232931276.1 radical SAM family heme chaperone HemW -
  L1S38_RS03910 (L1S38_03910) - 791237..791830 (-) 594 WP_059360147.1 XTP/dITP diphosphatase -
  L1S38_RS03915 (L1S38_03915) yggU 791834..792130 (-) 297 WP_059360148.1 DUF167 family protein YggU -
  L1S38_RS03920 (L1S38_03920) - 792127..792693 (-) 567 WP_008499757.1 YggT family protein -
  L1S38_RS03925 (L1S38_03925) - 792715..793416 (-) 702 WP_059360150.1 YggS family pyridoxal phosphate-dependent enzyme -
  L1S38_RS03930 (L1S38_03930) pilT 793434..794414 (+) 981 WP_059360151.1 type IV pilus twitching motility protein PilT Machinery gene
  L1S38_RS03935 (L1S38_03935) ruvX 794425..794841 (-) 417 WP_008499753.1 Holliday junction resolvase RuvX -
  L1S38_RS03940 (L1S38_03940) - 794841..795401 (-) 561 WP_008499752.1 YqgE/AlgH family protein -
  L1S38_RS03945 (L1S38_03945) gshB 795484..796431 (-) 948 WP_059360153.1 glutathione synthase -
  L1S38_RS03950 (L1S38_03950) rsmE 796451..797182 (-) 732 WP_021242075.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  L1S38_RS03955 (L1S38_03955) endA 797234..797941 (-) 708 WP_021242074.1 deoxyribonuclease I -
  L1S38_RS03960 (L1S38_03960) - 798036..798533 (-) 498 WP_059360154.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35525.56 Da        Isoelectric Point: 7.1631

>NTDB_id=647546 L1S38_RS03930 WP_059360151.1 793434..794414(+) (pilT) [Enterobacter roggenkampii strain GD21SC1505]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRTGRLEPAPFPSPDLGALLKVWLNDEQQGIWWANGQVDFAATVAGGQRLRG
SAFKQMRGVSVTLRLLPRTCPRLASLGAPRVIPELLTNASGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FIYQSARCLIQQREIGQHSPSFADALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLVPDLQDGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RSQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=647546 L1S38_RS03930 WP_059360151.1 793434..794414(+) (pilT) [Enterobacter roggenkampii strain GD21SC1505]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCTCG
CTGGCGCAGAACGGGCCGCCTTGAACCGGCACCGTTTCCCTCTCCGGACCTCGGGGCGTTGTTAAAAGTGTGGCTCAACG
ATGAGCAGCAGGGGATATGGTGGGCCAACGGCCAGGTGGATTTTGCCGCGACGGTGGCGGGAGGCCAGCGCCTGCGCGGC
AGTGCCTTTAAGCAGATGCGCGGAGTCTCTGTAACGCTACGGCTGTTGCCGCGTACCTGTCCGCGGCTCGCTTCGCTGGG
CGCACCGAGGGTTATCCCGGAACTGTTAACCAATGCGTCCGGGTTGATTCTGGTGACGGGGGCGACCGGCAGCGGCAAAT
CCACGACGCTGGCGGCGATGGTCGATTTTCTCAACCACCATACTGACGGCCATATTCTCACGCTCGAAGATCCGGTGGAG
TTTATCTATCAGAGCGCACGTTGCCTGATCCAGCAGCGGGAGATCGGCCAGCACAGCCCGTCATTTGCCGATGCGCTGCG
CAGTGCCTTACGCCAGGATCCGGACGTGATTTTACTGGGAGAGTTGCGCGACAGCGAAACGATCCGCCTGGCGCTAACGG
CAGCGGAGACCGGGCATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAGCGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAGAAGCTGGTTCC
TGACTTACAGGACGGGCGCGTCGCGCTGTATGAGCTGCTGGTGAATACCGCGGCGGCGGCGAATCTGATACGAGAAGGAA
AAACGTGGCAGCTGCCCGGCATTATTCAAACCGGACAGCAGGCAGGCATGCAGAACTTTGACCAAAGCCTGGCGGAGAGA
CGGTCGCAGGGGCGGTTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(86-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Legionella pneumophila strain Lp02

48.137

98.773

0.475
  pilT Legionella pneumophila strain ERS1305867

48.137

98.773

0.475
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Pseudomonas stutzeri DSM 10701

48.125

98.16

0.472
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

47.5

98.16

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

47.188

98.16

0.463
  pilT Acinetobacter baumannii D1279779

47.188

98.16

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.963

99.08

0.436
  pilU Pseudomonas stutzeri DSM 10701

40.181

100

0.408
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38
  pilU Acinetobacter baylyi ADP1

37.152

99.08

0.368