Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L0M13_RS07620 Genome accession   NZ_CP090977
Coordinates   1442987..1444027 (+) Length   346 a.a.
NCBI ID   WP_235198982.1    Uniprot ID   -
Organism   Planococcus sp. 107-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1438670..1462033 1442987..1444027 within 0


Gene organization within MGE regions


Location: 1438670..1462033
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0M13_RS07600 (L0M13_07600) - 1438670..1439086 (-) 417 WP_235198978.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  L0M13_RS07605 (L0M13_07605) - 1439428..1439817 (+) 390 WP_235198979.1 hypothetical protein -
  L0M13_RS07610 (L0M13_07610) - 1439819..1441303 (+) 1485 WP_235198980.1 VanW family protein -
  L0M13_RS07615 (L0M13_07615) - 1441326..1442984 (+) 1659 WP_235198981.1 GspE/PulE family protein -
  L0M13_RS07620 (L0M13_07620) pilT 1442987..1444027 (+) 1041 WP_235198982.1 type IV pilus twitching motility protein PilT Machinery gene
  L0M13_RS07625 (L0M13_07625) - 1444031..1445242 (+) 1212 WP_235198983.1 type II secretion system F family protein -
  L0M13_RS07630 (L0M13_07630) - 1445377..1445853 (+) 477 WP_235198984.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  L0M13_RS07635 (L0M13_07635) pilM 1445893..1446831 (+) 939 WP_235198985.1 type IV pilus biogenesis protein PilM -
  L0M13_RS07640 (L0M13_07640) - 1446834..1447166 (+) 333 WP_235198986.1 hypothetical protein -
  L0M13_RS07645 (L0M13_07645) - 1447207..1447596 (+) 390 WP_235198987.1 hypothetical protein -
  L0M13_RS07650 (L0M13_07650) - 1447730..1448554 (+) 825 WP_235198988.1 LysM peptidoglycan-binding domain-containing protein -
  L0M13_RS07655 (L0M13_07655) - 1448669..1449424 (+) 756 WP_235198989.1 A24 family peptidase -
  L0M13_RS07660 (L0M13_07660) - 1449620..1450234 (+) 615 WP_235198990.1 nucleoside triphosphate pyrophosphatase -
  L0M13_RS07665 (L0M13_07665) radC 1450245..1450781 (+) 537 Protein_1473 DNA repair protein RadC -
  L0M13_RS07670 (L0M13_07670) - 1450915..1452000 (+) 1086 WP_002333499.1 tyrosine-type recombinase/integrase -
  L0M13_RS07675 (L0M13_07675) - 1451997..1453889 (+) 1893 WP_016631285.1 site-specific integrase -
  L0M13_RS07680 (L0M13_07680) - 1453896..1454273 (+) 378 WP_016631286.1 DUF6262 family protein -
  L0M13_RS07685 (L0M13_07685) lnu(G) 1454654..1455457 (+) 804 WP_025481929.1 lincosamide nucleotidyltransferase Lnu(G) -
  L0M13_RS07690 (L0M13_07690) - 1455591..1455722 (+) 132 Protein_1478 JAB domain-containing protein -
  L0M13_RS07695 (L0M13_07695) - 1455812..1456840 (+) 1029 WP_311195639.1 rod shape-determining protein -
  L0M13_RS07700 (L0M13_07700) mreC 1456855..1457739 (+) 885 WP_235198992.1 rod shape-determining protein MreC -
  L0M13_RS07705 (L0M13_07705) mreD 1457736..1458280 (+) 545 Protein_1481 rod shape-determining protein MreD -
  L0M13_RS07710 (L0M13_07710) minC 1458315..1458980 (+) 666 WP_235198993.1 septum site-determining protein MinC -
  L0M13_RS07715 (L0M13_07715) minD 1458982..1459782 (+) 801 WP_235198994.1 septum site-determining protein MinD -
  L0M13_RS07720 (L0M13_07720) - 1459882..1460820 (+) 939 WP_235198995.1 nuclease-related domain-containing protein -
  L0M13_RS07725 (L0M13_07725) rplU 1461088..1461396 (+) 309 WP_235198996.1 50S ribosomal protein L21 -
  L0M13_RS07730 (L0M13_07730) - 1461400..1461729 (+) 330 WP_235198997.1 ribosomal-processing cysteine protease Prp -
  L0M13_RS07735 (L0M13_07735) rpmA 1461743..1462033 (+) 291 WP_008431505.1 50S ribosomal protein L27 -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38087.67 Da        Isoelectric Point: 6.2832

>NTDB_id=646867 L0M13_RS07620 WP_235198982.1 1442987..1444027(+) (pilT) [Planococcus sp. 107-1]
MPNDYINKVLTAAQEKNVSDIHITVGIPPVFRMHGTLQRFGDESITPELSKEIGKALIPETLWDVFLEKGEMDYSYSLPG
VARFRVNSFQQRGSISHAFRMIPTSIPTIEDLNMPPTLKNLAEINQGLILVTGPTGSGKSTTLAAMIRHINEKMNKHIIT
LEDPIEYLHQHGTSIIDQREVGFDTDSFANGLRAALRQDPDVILVGEMRDLETISTAITAAETGHLVMATLHTSSAASTI
ERIIDVFPPEQQSQVRTQLGGVLKAVVAQRLLPTADNKGRVAATEIMINNTAIANLIRTEKVHQIPNVILTNRSAGMHMM
LTTIQSLLSQGKISRQTAQPFLEGEE

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=646867 L0M13_RS07620 WP_235198982.1 1442987..1444027(+) (pilT) [Planococcus sp. 107-1]
ATGCCAAATGATTATATCAACAAAGTATTAACGGCTGCCCAGGAAAAAAATGTATCCGATATTCACATTACAGTTGGAAT
TCCACCGGTATTTCGAATGCACGGAACACTTCAGCGATTCGGGGACGAAAGCATCACGCCGGAACTTTCAAAAGAAATTG
GGAAAGCATTAATCCCAGAGACATTATGGGACGTTTTTCTAGAAAAAGGCGAAATGGACTACTCCTACTCGTTGCCTGGA
GTTGCCAGATTCCGAGTCAACTCATTCCAGCAGCGAGGTTCGATTTCGCATGCGTTCCGAATGATTCCGACTAGTATTCC
AACAATTGAAGATTTGAACATGCCGCCGACGCTGAAGAACTTGGCTGAGATTAATCAAGGATTAATCTTGGTGACTGGTC
CTACAGGCTCTGGTAAATCAACTACGCTTGCTGCGATGATCCGCCACATTAATGAAAAAATGAATAAACATATCATCACC
CTTGAAGATCCGATTGAATACTTGCATCAGCACGGAACATCGATTATTGATCAACGGGAAGTCGGTTTTGACACAGATAG
CTTCGCAAATGGTTTGCGGGCAGCGTTGCGTCAAGATCCAGACGTGATTTTAGTCGGAGAAATGCGAGACCTTGAAACCA
TTTCAACCGCTATTACAGCTGCAGAAACTGGCCACTTGGTTATGGCGACGCTCCATACTTCAAGCGCTGCTTCTACTATC
GAGCGGATTATCGACGTCTTCCCTCCGGAACAGCAATCCCAAGTGCGTACACAGCTTGGCGGTGTGTTAAAAGCTGTAGT
GGCCCAACGTTTGCTGCCGACTGCCGACAATAAAGGACGCGTTGCCGCAACGGAAATCATGATCAATAACACCGCGATCG
CCAATTTAATACGTACCGAAAAAGTCCATCAGATTCCGAATGTTATATTAACGAATCGTTCTGCAGGCATGCATATGATG
CTGACGACAATTCAAAGCTTGCTTTCCCAAGGGAAAATTTCCAGGCAGACAGCGCAGCCTTTCCTGGAAGGAGAGGAATA
G

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.415

98.844

0.488
  pilT Vibrio cholerae strain A1552

49.415

98.844

0.488
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.769

93.931

0.477
  pilT Acinetobacter nosocomialis M2

48.225

97.688

0.471
  pilT Neisseria meningitidis 8013

48.225

97.688

0.471
  pilT Acinetobacter baumannii D1279779

47.929

97.688

0.468
  pilT Neisseria gonorrhoeae MS11

47.929

97.688

0.468
  pilT Pseudomonas stutzeri DSM 10701

47.929

97.688

0.468
  pilT Acinetobacter baumannii strain A118

47.929

97.688

0.468
  pilT Legionella pneumophila strain Lp02

47.929

97.688

0.468
  pilT Legionella pneumophila strain ERS1305867

47.929

97.688

0.468
  pilT Acinetobacter baylyi ADP1

46.667

99.711

0.465
  pilT Pseudomonas aeruginosa PAK

47.041

97.688

0.46
  pilU Pseudomonas stutzeri DSM 10701

44.109

95.665

0.422
  pilU Vibrio cholerae strain A1552

40.476

97.11

0.393
  pilU Acinetobacter baylyi ADP1

38.623

96.532

0.373