Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L0B52_RS02505 Genome accession   NZ_CP090975
Coordinates   525894..526940 (-) Length   348 a.a.
NCBI ID   WP_235064966.1    Uniprot ID   -
Organism   Suttonella sp. R2A3     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 520894..531940
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0B52_RS02495 (L0B52_02495) parE 522696..524615 (-) 1920 WP_235064964.1 DNA topoisomerase IV subunit B -
  L0B52_RS02500 (L0B52_02500) - 524668..525885 (-) 1218 WP_235064965.1 PilT/PilU family type 4a pilus ATPase -
  L0B52_RS02505 (L0B52_02505) pilT 525894..526940 (-) 1047 WP_235064966.1 type IV pilus twitching motility protein PilT Machinery gene
  L0B52_RS02510 (L0B52_02510) proC 527053..527898 (+) 846 WP_235064967.1 pyrroline-5-carboxylate reductase -
  L0B52_RS02515 (L0B52_02515) - 527900..528418 (+) 519 WP_235064968.1 YggT family protein -
  L0B52_RS02520 (L0B52_02520) mnmG 528424..530286 (+) 1863 WP_235065424.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG -
  L0B52_RS02525 (L0B52_02525) - 530283..530483 (+) 201 WP_235064969.1 hypothetical protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38832.45 Da        Isoelectric Point: 6.6432

>NTDB_id=646827 L0B52_RS02505 WP_235064966.1 525894..526940(-) (pilT) [Suttonella sp. R2A3]
MASKTLLDDLLRFAHQNNASDLHLSSGLPPIVRVDGDIKRLNMPELDAQELRNMVYDIMANSMVRDFEETWEADFSTEIR
GISRFRVNVFNQNRGIAVVFRIIPSKVLSLRELGAPEKFVDIIDVPRGLVLVTGPTGSGKSTTLAAMLDHINNTKHEHIL
TIEDPIEFVHQSNKSLINQREVYRHTQSFNNALRAALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKT
IDRIIDVFPGEEKQLVRSMLSESLRAVIAQTLLKKQTGGRVAAHEVLVGTPAVKNLIREDKVAQIYSMIQTGAQHGMQTL
DQSLQDLVMKGVVDSEHARTKAQDKYSF

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=646827 L0B52_RS02505 WP_235064966.1 525894..526940(-) (pilT) [Suttonella sp. R2A3]
ATGGCCAGCAAAACTTTGTTAGATGATTTATTGCGTTTTGCCCATCAAAATAATGCCTCAGATTTGCATTTAAGCTCCGG
CTTACCGCCAATAGTGCGTGTTGATGGGGATATCAAGCGTCTTAACATGCCTGAGCTTGATGCGCAAGAGCTGCGCAATA
TGGTTTATGACATTATGGCCAATTCGATGGTGCGTGATTTTGAGGAAACGTGGGAAGCGGATTTTTCCACCGAAATTCGC
GGTATTTCGCGTTTTCGTGTCAATGTGTTTAATCAAAACCGTGGGATCGCCGTTGTTTTCCGGATTATTCCTAGCAAAGT
ACTTTCCTTACGGGAGCTTGGTGCGCCGGAGAAATTTGTTGATATCATCGATGTGCCGCGTGGATTGGTGCTGGTTACTG
GTCCGACGGGTTCGGGTAAGTCAACCACTTTGGCGGCGATGCTCGATCATATTAATAACACCAAACACGAACATATCCTC
ACCATCGAAGATCCGATTGAATTCGTCCATCAAAGCAATAAGTCTTTGATTAACCAGCGTGAAGTTTATCGTCATACGCA
AAGTTTTAATAACGCCTTGCGCGCGGCGCTTCGTGAGGACCCGGATATTATTCTGGTTGGTGAGCTGCGTGATTTAGAAA
CGATTCGTTTAGCGCTCACTGCTGCCGAAACAGGCCATTTAGTCTTTGGTACACTGCATACCAGTTCAGCGGCCAAAACC
ATCGATCGGATTATCGATGTGTTCCCTGGCGAAGAAAAGCAATTGGTTCGTTCGATGCTTTCTGAGTCACTTCGCGCGGT
TATCGCACAAACCTTACTCAAAAAACAAACTGGTGGGCGTGTTGCAGCGCATGAAGTGTTAGTCGGTACGCCTGCAGTGA
AAAACTTGATTCGTGAGGATAAGGTTGCACAAATCTATTCGATGATTCAAACCGGTGCGCAGCACGGTATGCAAACCTTA
GACCAATCGTTGCAGGATTTGGTGATGAAAGGGGTTGTCGATTCAGAGCATGCGCGCACTAAAGCCCAAGATAAATACTC
ATTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

69.006

98.276

0.678
  pilT Acinetobacter baumannii D1279779

68.129

98.276

0.67
  pilT Acinetobacter baumannii strain A118

68.129

98.276

0.67
  pilT Acinetobacter nosocomialis M2

68.129

98.276

0.67
  pilT Pseudomonas stutzeri DSM 10701

68.129

98.276

0.67
  pilT Pseudomonas aeruginosa PAK

68.129

98.276

0.67
  pilT Neisseria meningitidis 8013

64.14

98.563

0.632
  pilT Neisseria gonorrhoeae MS11

63.848

98.563

0.629
  pilT Legionella pneumophila strain Lp02

63.529

97.701

0.621
  pilT Legionella pneumophila strain ERS1305867

63.529

97.701

0.621
  pilT Vibrio cholerae strain A1552

62.985

96.264

0.606
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

62.985

96.264

0.606
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.289

100

0.514
  pilU Pseudomonas stutzeri DSM 10701

39.64

95.69

0.379
  pilU Vibrio cholerae strain A1552

39.157

95.402

0.374
  pilU Acinetobacter baylyi ADP1

39.319

92.816

0.365