Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LY254_RS08280 Genome accession   NZ_CP090898
Coordinates   1575234..1576307 (-) Length   357 a.a.
NCBI ID   WP_010313994.1    Uniprot ID   -
Organism   Synechococcus sp. NB0720_010     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1570234..1581307
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LY254_RS08260 (LY254_08260) - 1571170..1571721 (+) 552 WP_247476581.1 isoprenylcysteine carboxylmethyltransferase family protein -
  LY254_RS08265 (LY254_08265) dusA 1571726..1572724 (-) 999 WP_247476583.1 tRNA dihydrouridine(20/20a) synthase DusA -
  LY254_RS08270 (LY254_08270) - 1572750..1574000 (+) 1251 WP_247476584.1 NAD(P)/FAD-dependent oxidoreductase -
  LY254_RS08275 (LY254_08275) - 1573993..1575234 (-) 1242 WP_247476586.1 type II secretion system F family protein -
  LY254_RS08280 (LY254_08280) pilT 1575234..1576307 (-) 1074 WP_010313994.1 type IV pilus twitching motility protein PilT Machinery gene
  LY254_RS08285 (LY254_08285) - 1576374..1578197 (-) 1824 WP_247476588.1 GspE/PulE family protein -
  LY254_RS08290 (LY254_08290) grpE 1578293..1578967 (+) 675 WP_010313990.1 nucleotide exchange factor GrpE -
  LY254_RS08295 (LY254_08295) dnaJ 1578970..1580097 (+) 1128 WP_247476589.1 molecular chaperone DnaJ -
  LY254_RS08300 (LY254_08300) - 1580105..1580326 (+) 222 WP_247476591.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 39282.76 Da        Isoelectric Point: 6.5036

>NTDB_id=646221 LY254_RS08280 WP_010313994.1 1575234..1576307(-) (pilT) [Synechococcus sp. NB0720_010]
MELQIEQLMEELVEAGGSDLHLAAGQPPYGRFSGALKPMREERLSEDLCNRLIFSMINNAQRKQLEQTWELDCAYGLKGV
SRFRVNVYRQRGSYAACLRALGSTIPSLESLGLPPIVEETSRRPRGLVLVTGPTGSGKTTTLAALLDHINRSRAEHILTI
EDPIEFTYRNDKSVIHQRQLGDDTRSFGAALRAALREDPDVILVGEMRDLETIQLAITASETGHLVFGTLHTSSAAQTVD
RMVDVFPPEQQTQIRVQLSGSLVGVFSQTLCKRQNPKPGQFGRVMAQEILINTPAIANLIREGKTAQLYSQIQTGGQQGM
QTLERALANLVQAGAVERSEALAKASKPDELQRLLEG

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=646221 LY254_RS08280 WP_010313994.1 1575234..1576307(-) (pilT) [Synechococcus sp. NB0720_010]
ATGGAGCTCCAGATCGAACAGTTGATGGAGGAGCTGGTCGAGGCGGGTGGCAGCGACTTGCACCTGGCGGCCGGCCAACC
GCCCTATGGCCGCTTCAGTGGGGCGCTCAAACCGATGCGGGAGGAGCGGCTCAGCGAGGACCTCTGCAACCGGTTGATCT
TCTCGATGATCAACAACGCCCAGCGCAAGCAGCTGGAGCAAACCTGGGAGCTGGACTGCGCCTATGGCCTCAAAGGCGTC
TCCCGCTTTCGGGTCAATGTCTACCGCCAGCGGGGGAGCTATGCGGCCTGTCTGCGGGCCCTCGGCAGCACGATCCCCAG
CCTGGAGAGCCTGGGCTTACCGCCCATCGTCGAGGAAACCAGCCGCCGCCCCAGGGGTCTGGTGCTGGTGACCGGTCCCA
CCGGCTCAGGCAAAACCACGACGCTGGCGGCCCTGCTGGATCACATCAACCGCAGCCGCGCCGAGCACATCCTCACGATC
GAAGATCCGATCGAATTCACCTACCGCAACGACAAAAGCGTGATCCACCAGCGCCAGCTGGGGGATGACACCCGCAGCTT
TGGCGCCGCCCTTCGCGCCGCCCTGCGGGAGGACCCCGACGTGATCCTGGTGGGCGAAATGAGAGATCTGGAAACAATCC
AGTTGGCGATCACGGCCTCAGAGACGGGGCACCTGGTCTTTGGCACCCTGCACACCAGCTCGGCGGCGCAAACCGTCGAT
CGCATGGTGGATGTCTTCCCGCCAGAGCAGCAAACCCAGATCAGGGTTCAGCTCAGTGGCTCCCTGGTGGGGGTGTTCTC
GCAGACCCTCTGCAAACGCCAGAACCCCAAACCCGGGCAATTCGGGCGGGTCATGGCCCAAGAAATCCTGATCAACACCC
CGGCCATCGCCAACTTGATCCGGGAGGGCAAAACCGCCCAGCTCTATTCCCAGATCCAAACGGGCGGCCAGCAGGGGATG
CAAACCCTCGAGCGCGCCCTGGCGAACCTGGTGCAGGCCGGAGCAGTCGAGCGCAGCGAAGCCCTCGCCAAAGCGAGCAA
ACCCGATGAGCTGCAGCGGCTCCTGGAGGGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-246)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.576

97.759

0.504
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.284

98.599

0.496
  pilT Pseudomonas stutzeri DSM 10701

50.43

97.759

0.493
  pilT Acinetobacter baylyi ADP1

50.43

97.759

0.493
  pilT Acinetobacter baumannii D1279779

50.143

97.759

0.49
  pilT Acinetobacter nosocomialis M2

50.143

97.759

0.49
  pilT Acinetobacter baumannii strain A118

50.143

97.759

0.49
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.585

95.798

0.485
  pilT Vibrio cholerae strain A1552

50.585

95.798

0.485
  pilT Legionella pneumophila strain ERS1305867

51.032

94.958

0.485
  pilT Legionella pneumophila strain Lp02

51.032

94.958

0.485
  pilT Neisseria gonorrhoeae MS11

48.138

97.759

0.471
  pilT Neisseria meningitidis 8013

50

93.557

0.468
  pilU Vibrio cholerae strain A1552

37.288

99.16

0.37
  pilU Pseudomonas stutzeri DSM 10701

37.288

99.16

0.37
  pilU Acinetobacter baylyi ADP1

36.544

98.88

0.361