Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L0992_RS13770 Genome accession   NZ_CP090854
Coordinates   3150086..3151195 (-) Length   369 a.a.
NCBI ID   WP_373948887.1    Uniprot ID   -
Organism   Vibrio pomeroyi strain YSX02     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3145086..3156195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0992_RS13735 (L0992_13730) - 3145358..3145858 (+) 501 WP_373948879.1 SprT family zinc-dependent metalloprotease -
  L0992_RS13740 (L0992_13735) - 3146010..3146741 (+) 732 WP_373948881.1 endonuclease -
  L0992_RS13745 (L0992_13740) rsmE 3146895..3147626 (+) 732 WP_210443101.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  L0992_RS13750 (L0992_13745) gshB 3147636..3148586 (+) 951 WP_373948884.1 glutathione synthase -
  L0992_RS13755 (L0992_13750) - 3148696..3149259 (+) 564 WP_017629552.1 YqgE/AlgH family protein -
  L0992_RS13760 (L0992_13755) ruvX 3149314..3149736 (+) 423 WP_048659417.1 Holliday junction resolvase RuvX -
  L0992_RS13765 (L0992_13760) - 3149835..3149996 (+) 162 WP_176680464.1 hypothetical protein -
  L0992_RS13770 (L0992_13765) pilU 3150086..3151195 (-) 1110 WP_373948887.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L0992_RS13775 (L0992_13770) pilT 3151210..3152247 (-) 1038 WP_017629549.1 type IV pilus twitching motility protein PilT Machinery gene
  L0992_RS13780 (L0992_13775) - 3152274..3152978 (+) 705 WP_055319638.1 YggS family pyridoxal phosphate-dependent enzyme -
  L0992_RS13785 (L0992_13780) proC 3153063..3153881 (+) 819 WP_373948890.1 pyrroline-5-carboxylate reductase -
  L0992_RS13790 (L0992_13785) - 3153940..3154497 (+) 558 WP_017067798.1 YggT family protein -
  L0992_RS13795 (L0992_13790) yggU 3154527..3154817 (+) 291 WP_009847703.1 DUF167 family protein YggU -
  L0992_RS13800 (L0992_13795) - 3154846..3155277 (+) 432 WP_061018830.1 DUF4426 domain-containing protein -
  L0992_RS13805 (L0992_13800) - 3155324..3155923 (+) 600 WP_373948893.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41323.19 Da        Isoelectric Point: 5.8700

>NTDB_id=645267 L0992_RS13770 WP_373948887.1 3150086..3151195(-) (pilU) [Vibrio pomeroyi strain YSX02]
MELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGDKLNAAQVAQLLDAMMDQDRRDEYQQTREANFAIVREFGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGSGRHGVFEILLNSPRVSDLIRRGELHELKATMAKSKEIGMQTFDQALY
DLVVGGKISEEDAFHSADSANDLRLMLKTRRGDDDYGTGALAGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=645267 L0992_RS13770 WP_373948887.1 3150086..3151195(-) (pilU) [Vibrio pomeroyi strain YSX02]
ATGGAATTGAATCAAATCCTTGAAGGGATGCTGTCTCAAAAAGCATCGGATCTTTATATCACTGTTGATGCACCTATCCT
GTTCCGTGTGGATGGTGAATTACGACCTCAAGGCGATAAGCTGAATGCGGCTCAGGTTGCTCAATTACTTGATGCGATGA
TGGATCAAGACCGACGTGATGAATATCAGCAAACACGTGAAGCAAACTTTGCCATTGTGCGTGAGTTTGGTCGCTTTCGT
GTCAGTGCATTCTTTCAACGAGAACTGCCTGGAGCGGTCATTCGACGCATCGAGACTAACATCCCAACCTTTGAGCAATT
AAAGCTTCCTGATGTATTACAAGACCTTTCAATCGCCAAGCGTGGGCTTGTGCTGGTGGTTGGTGCAACTGGCTCTGGTA
AATCGACCTCGATGGCTGCGATGACAGGCTATCGCAATACCAATCGTTCAGGTCATATCTTAACAGTTGAAGACCCGATT
GAGTTCGTCCATGAACATAAAAAATGTATTGTGACTCAGCGTGAGGTAGGGCTCGACACCGAGAGTTATGAAGTCGCACT
TAAGAACTCTTTGCGCCAAGCACCAGATATGATTTTGATTGGCGAAATCCGTAGCCGTGAAACCATGGAATACGCGATGA
CCTTTGCTGAAACGGGTCACTTGTGTATGGCGACACTGCACGCGAATAATGCCAACCAAGCGTTAGAGCGTATTCTCCAC
TTGGTGCCGAAAGAGCAGAAAGAACAGTTCCTGTTTGATCTGTCGATGAATCTGCGTGGTGTCGTCGCTCAGCAGTTAAT
CCGAGATAAGAATGGCAGCGGTCGTCATGGTGTGTTTGAGATTTTACTCAACAGCCCACGAGTGTCTGACTTGATTCGTC
GCGGTGAACTGCACGAGCTAAAAGCAACAATGGCGAAATCGAAAGAGATTGGGATGCAGACCTTTGACCAAGCTTTGTAT
GATTTAGTTGTAGGAGGCAAGATCAGCGAAGAGGATGCGTTCCACAGTGCTGACTCCGCTAACGACTTACGCTTGATGCT
AAAAACTAGACGTGGCGATGATGACTATGGGACTGGTGCTTTGGCTGGTGTGAAGATCGATATGGGGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.239

99.729

0.84
  pilU Pseudomonas stutzeri DSM 10701

56.334

100

0.566
  pilU Acinetobacter baylyi ADP1

54.986

95.122

0.523
  pilT Acinetobacter baumannii strain A118

42.353

92.141

0.39
  pilT Acinetobacter baumannii D1279779

42.353

92.141

0.39
  pilT Acinetobacter nosocomialis M2

42.353

92.141

0.39
  pilT Legionella pneumophila strain ERS1305867

42.183

91.87

0.388
  pilT Legionella pneumophila strain Lp02

42.183

91.87

0.388
  pilT Acinetobacter baylyi ADP1

42.169

89.973

0.379
  pilT Pseudomonas aeruginosa PAK

41.791

90.786

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.593

87.805

0.374
  pilT Pseudomonas stutzeri DSM 10701

40.299

90.786

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Neisseria gonorrhoeae MS11

38.841

93.496

0.363
  pilT Neisseria meningitidis 8013

38.841

93.496

0.363