Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L0994_RS02115 Genome accession   NZ_CP090847
Coordinates   422433..423470 (+) Length   345 a.a.
NCBI ID   WP_373929932.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain WXL032     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 417433..428470
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0994_RS02080 (L0994_02075) hemW 417520..418707 (-) 1188 WP_010435644.1 radical SAM family heme chaperone HemW -
  L0994_RS02085 (L0994_02080) - 418707..419306 (-) 600 WP_010435646.1 XTP/dITP diphosphatase -
  L0994_RS02090 (L0994_02085) - 419355..419786 (-) 432 WP_016768173.1 DUF4426 domain-containing protein -
  L0994_RS02095 (L0994_02090) yggU 419817..420107 (-) 291 WP_010435651.1 DUF167 family protein YggU -
  L0994_RS02100 (L0994_02095) - 420137..420694 (-) 558 WP_010435653.1 YggT family protein -
  L0994_RS02105 (L0994_02100) proC 420771..421589 (-) 819 WP_010435654.1 pyrroline-5-carboxylate reductase -
  L0994_RS02110 (L0994_02105) - 421702..422406 (-) 705 WP_010435657.1 YggS family pyridoxal phosphate-dependent enzyme -
  L0994_RS02115 (L0994_02110) pilT 422433..423470 (+) 1038 WP_373929932.1 type IV pilus twitching motility protein PilT Machinery gene
  L0994_RS02120 (L0994_02115) pilU 423485..424594 (+) 1110 WP_010435664.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L0994_RS02125 (L0994_02120) - 424651..424812 (-) 162 WP_193838523.1 hypothetical protein -
  L0994_RS02130 (L0994_02125) ruvX 424911..425333 (-) 423 WP_004735480.1 Holliday junction resolvase RuvX -
  L0994_RS02135 (L0994_02130) - 425382..425945 (-) 564 WP_016768176.1 YqgE/AlgH family protein -
  L0994_RS02140 (L0994_02135) gshB 426062..427012 (-) 951 WP_004735482.1 glutathione synthase -
  L0994_RS02145 (L0994_02140) rsmE 427022..427753 (-) 732 WP_016783757.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38192.73 Da        Isoelectric Point: 6.0495

>NTDB_id=645204 L0994_RS02115 WP_373929932.1 422433..423470(+) (pilT) [Vibrio cyclitrophicus strain WXL032]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMDDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQARGCAAVFRTIPVEIPTLDQLGAPEIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLVNQREVHRDTHTFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIAQGLVDSDEVEKKIEIQASMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=645204 L0994_RS02115 WP_373929932.1 422433..423470(+) (pilT) [Vibrio cyclitrophicus strain WXL032]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATACCAGCTTTGAGTCATGCTGATGTACATCGTTTAGTTTTTGAGA
TCATGGACGATTCACAACGCGGTGAGTTTGAGGAAAAATTGGAAGTCGACTTCTCTTTTGAATTACCCAATGTTGGTCGC
TTCCGTGTTAATGCTTTTAATCAAGCTCGTGGGTGTGCTGCTGTCTTTCGAACTATCCCTGTAGAGATCCCGACTTTAGA
TCAGCTTGGTGCGCCAGAAATCTTTGAAAAGATTGCGAACTACGAGAAAGGTTTAGTGCTTGTCACTGGCCCTACAGGTT
CTGGTAAATCGACAACTTTGGCGGCGATGGTGGATTACGTAAACCGCAATCACAACAAACACATTTTGACGATTGAAGAT
CCAATTGAATTCGTTCACACCAATAATAAATGCCTAGTAAACCAGCGTGAGGTTCATCGTGATACTCACACCTTTAAAGC
GGCGTTGCGCAGTGCATTACGTGAGGATCCGGACGTGATTCTTGTTGGTGAACTTCGTGACCAAGAGACGATCAGCTTAG
CGTTAACCGCAGCTGAAACGGGTCACTTAGTTTTTGGTACTTTGCATACAAGCTCTGCGGCAAAAACTATCGACCGTATT
ATCGACGTTTTCCCAGGTAGCGACAAAGATATGGTTCGTTCAATGTTATCTGAATCACTACGCTCGGTGATTGCTCAAAA
GCTGTTAAAACGTGTTGGTGGTGGTCGTGTGGCTTGTCATGAAATAATGATGGCGACACCTGCAATTAGAAACTTGATCC
GTGAAGACAAAGTCGCGCAGATGTACTCGATCATTCAAACGGGTGCCGCACATGGCATGCAAACCATGGAGCAAAATGCG
AAGCAATTGATCGCGCAAGGTTTGGTTGATTCGGACGAGGTCGAGAAAAAGATCGAAATTCAAGCCTCTATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.696

100

0.887
  pilT Vibrio cholerae strain A1552

88.696

100

0.887
  pilT Acinetobacter baumannii D1279779

72.644

95.362

0.693
  pilT Acinetobacter baumannii strain A118

72.644

95.362

0.693
  pilT Acinetobacter nosocomialis M2

72.34

95.362

0.69
  pilT Pseudomonas stutzeri DSM 10701

69.733

97.681

0.681
  pilT Acinetobacter baylyi ADP1

70.909

95.652

0.678
  pilT Pseudomonas aeruginosa PAK

68.529

98.551

0.675
  pilT Legionella pneumophila strain Lp02

67.781

95.362

0.646
  pilT Legionella pneumophila strain ERS1305867

67.781

95.362

0.646
  pilT Neisseria meningitidis 8013

62.319

100

0.623
  pilT Neisseria gonorrhoeae MS11

62.029

100

0.62
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.841

0.496
  pilU Vibrio cholerae strain A1552

43.077

94.203

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.854

95.072

0.388
  pilU Acinetobacter baylyi ADP1

39.688

92.754

0.368