Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L0Y28_RS04165 Genome accession   NZ_CP090589
Coordinates   918247..919263 (+) Length   338 a.a.
NCBI ID   WP_256542896.1    Uniprot ID   -
Organism   Pectobacterium aroidearum strain QJ316     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 913247..924263
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0Y28_RS04130 (L0Y28_04130) - 913475..913735 (-) 261 WP_256543388.1 type II toxin-antitoxin system ParD family antitoxin -
  L0Y28_RS04135 (L0Y28_04135) hemW 913964..915106 (-) 1143 WP_256542895.1 radical SAM family heme chaperone HemW -
  L0Y28_RS04140 (L0Y28_04140) - 915099..915692 (-) 594 WP_014916538.1 XTP/dITP diphosphatase -
  L0Y28_RS04145 (L0Y28_04145) yggU 915710..916000 (-) 291 WP_010279110.1 DUF167 family protein YggU -
  L0Y28_RS04150 (L0Y28_04150) - 915997..916551 (-) 555 WP_010295003.1 YggT family protein -
  L0Y28_RS04155 (L0Y28_04155) proC 916600..917421 (-) 822 WP_015841592.1 pyrroline-5-carboxylate reductase -
  L0Y28_RS04160 (L0Y28_04160) - 917512..918225 (-) 714 WP_015841591.1 YggS family pyridoxal phosphate-dependent enzyme -
  L0Y28_RS04165 (L0Y28_04165) pilT 918247..919263 (+) 1017 WP_256542896.1 type IV pilus twitching motility protein PilT Machinery gene
  L0Y28_RS04170 (L0Y28_04170) - 919232..921058 (-) 1827 WP_256542897.1 diguanylate cyclase -
  L0Y28_RS04175 (L0Y28_04175) - 921586..923157 (-) 1572 WP_256542898.1 methyl-accepting chemotaxis protein -
  L0Y28_RS04180 (L0Y28_04180) nsrR 923474..923899 (+) 426 WP_015841587.1 nitric oxide-sensing transcriptional repressor NsrR -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36889.10 Da        Isoelectric Point: 6.7684

>NTDB_id=643671 L0Y28_RS04165 WP_256542896.1 918247..919263(+) (pilT) [Pectobacterium aroidearum strain QJ316]
MELDEWIARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTSEQVAQWCDAWLEPAQREQLRQFGQIDGALMLPDGQ
RLRVNVFRQREGLSAALRIIPSVQPSLDGLHAPPVLSTLLEKTNGLILITGATGSGKSTTLAAMIGALNDSSDRHVITLE
DPIEFIHASRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQVVDR
LIDVFPGEEKAYVRSQLSTCLQAVVTQRLLPSAHGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGTAAGMQTFEQS
YQQRCRDGLISHNCALAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=643671 L0Y28_RS04165 WP_256542896.1 918247..919263(+) (pilT) [Pectobacterium aroidearum strain QJ316]
ATGGAATTGGATGAGTGGATAGCGCGTAGTGTAAAACATAATGCCTCAGATCTGCACCTTTGTAGCGGCCACCCGCCGGT
GTTACGTGTCGATGGGCGGCTACAGCCTGAAAACACCTTACCACGTTTAACGTCGGAGCAGGTGGCACAATGGTGCGATG
CCTGGCTTGAACCCGCTCAGAGAGAACAACTGCGGCAGTTTGGTCAGATAGACGGCGCGTTAATGCTGCCTGATGGGCAG
CGTCTGCGGGTCAATGTGTTTCGCCAGCGAGAAGGGCTGTCCGCGGCGCTGCGTATTATCCCGTCCGTTCAGCCGTCATT
GGACGGGCTACACGCGCCACCCGTTCTGTCGACGCTGTTGGAAAAAACGAACGGGCTGATCCTGATTACCGGTGCGACGG
GGAGCGGTAAATCCACGACGCTGGCGGCAATGATCGGGGCGTTAAACGACAGCAGCGATCGCCATGTGATTACGCTGGAG
GATCCGATCGAGTTTATCCATGCCAGCCGACGCTGCCTGATTCAACAGCGTGAGATCGGTGCGCACAGCGCGTCTTTTGC
ACAGGCGCTACGGGCAGCCTTACGGGAAGATCCCGATGTGATTCTGCTGGGGGAATTGCGCGATACGGAGACGATTCGGT
TGGCGCTGACGGCAGCGGAAACCGGCCATCTGGTGCTATCCACGCTGCATACGCGCAGTGCATCGCAGGTGGTCGATCGT
CTGATTGACGTCTTTCCCGGTGAAGAGAAAGCCTATGTTCGCAGTCAGCTATCCACCTGTTTGCAGGCCGTGGTGACGCA
AAGGTTGTTACCTTCTGCACACGGCGGTCGCATTGCGCTTTATGAAGTGCTGACGGCGACAGCGGCAGTCAGCAACCTGA
TTCGGGAAGGGAAAACGCATCAGCTTCCCGGCCTGATTCAAACGGGAACGGCTGCGGGCATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAATTGCGCCCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

50.298

99.408

0.5
  pilT Vibrio cholerae strain A1552

51.212

97.633

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.212

97.633

0.5
  pilT Neisseria gonorrhoeae MS11

50

99.408

0.497
  pilT Legionella pneumophila strain ERS1305867

49.552

99.112

0.491
  pilT Legionella pneumophila strain Lp02

49.552

99.112

0.491
  pilT Pseudomonas stutzeri DSM 10701

48.955

99.112

0.485
  pilT Pseudomonas aeruginosa PAK

48.657

99.112

0.482
  pilT Acinetobacter baylyi ADP1

48.225

100

0.482
  pilT Acinetobacter nosocomialis M2

47.761

99.112

0.473
  pilT Acinetobacter baumannii D1279779

47.761

99.112

0.473
  pilT Acinetobacter baumannii strain A118

47.761

99.112

0.473
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.543

99.704

0.414
  pilU Pseudomonas stutzeri DSM 10701

40.653

99.704

0.405
  pilU Vibrio cholerae strain A1552

39.169

99.704

0.391
  pilU Acinetobacter baylyi ADP1

39.441

95.266

0.376