Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L0Y25_RS04365 Genome accession   NZ_CP090588
Coordinates   956606..957622 (+) Length   338 a.a.
NCBI ID   WP_256554359.1    Uniprot ID   -
Organism   Pectobacterium aroidearum strain AK042     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 951606..962622
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0Y25_RS04330 (L0Y25_04330) - 951835..952095 (-) 261 WP_256543388.1 type II toxin-antitoxin system ParD family antitoxin -
  L0Y25_RS04335 (L0Y25_04335) hemW 952323..953465 (-) 1143 WP_180778632.1 radical SAM family heme chaperone HemW -
  L0Y25_RS04340 (L0Y25_04340) - 953458..954051 (-) 594 WP_039470191.1 XTP/dITP diphosphatase -
  L0Y25_RS04345 (L0Y25_04345) yggU 954069..954359 (-) 291 WP_010279110.1 DUF167 family protein YggU -
  L0Y25_RS04350 (L0Y25_04350) - 954356..954910 (-) 555 WP_010295003.1 YggT family protein -
  L0Y25_RS04355 (L0Y25_04355) proC 954959..955780 (-) 822 WP_015841592.1 pyrroline-5-carboxylate reductase -
  L0Y25_RS04360 (L0Y25_04360) - 955871..956584 (-) 714 WP_256554358.1 YggS family pyridoxal phosphate-dependent enzyme -
  L0Y25_RS04365 (L0Y25_04365) pilT 956606..957622 (+) 1017 WP_256554359.1 type IV pilus twitching motility protein PilT Machinery gene
  L0Y25_RS04370 (L0Y25_04370) - 957591..959417 (-) 1827 WP_181845903.1 diguanylate cyclase -
  L0Y25_RS04375 (L0Y25_04375) - 959946..961517 (-) 1572 WP_180778637.1 methyl-accepting chemotaxis protein -
  L0Y25_RS04380 (L0Y25_04380) nsrR 961833..962258 (+) 426 WP_015841587.1 nitric oxide-sensing transcriptional repressor NsrR -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36887.08 Da        Isoelectric Point: 6.7684

>NTDB_id=643647 L0Y25_RS04365 WP_256554359.1 956606..957622(+) (pilT) [Pectobacterium aroidearum strain AK042]
MELDEWIARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTSEQVAQWCDAWLEPAQREQLRQFGQIDGALMLPDGQ
RLRVNVFRQREGLSAALRIIPSVQPSLDGLHAPPVLSTLLEKPNGLILITGATGSGKSTTLAAMLGALNDSSDRHVITLE
DPIEFIHASRRCLIQQREIGAHSASFAQALRATLREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLSTCLQAVVTQRLLPSAHGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGTAAGMQTFEQS
YQQRCRDGLISHNCALAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=643647 L0Y25_RS04365 WP_256554359.1 956606..957622(+) (pilT) [Pectobacterium aroidearum strain AK042]
ATGGAATTGGATGAGTGGATAGCGCGTAGTGTAAAACATAATGCCTCAGATCTGCACCTTTGTAGCGGCCACCCGCCAGT
GTTACGTGTCGATGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGTCAGAGCAGGTGGCACAATGGTGCGATG
CCTGGCTTGAACCCGCTCAGAGAGAACAACTGCGGCAGTTTGGTCAGATAGACGGCGCGTTAATGCTGCCTGATGGGCAG
CGTCTGCGAGTCAATGTGTTTCGCCAGCGAGAAGGGCTGTCTGCGGCGCTGCGTATTATCCCGTCCGTTCAGCCGTCATT
GGACGGGCTACACGCGCCACCCGTTCTGTCGACGCTGTTGGAGAAACCGAACGGGCTGATCCTGATTACCGGTGCGACGG
GGAGCGGTAAATCCACGACGCTGGCGGCAATGCTCGGGGCGTTAAATGACAGCAGCGATCGCCATGTGATTACGCTGGAG
GATCCGATCGAGTTTATCCATGCCAGCCGACGCTGCCTGATTCAACAGCGAGAGATCGGTGCGCACAGCGCGTCTTTTGC
ACAGGCGCTACGGGCCACCTTGCGGGAAGATCCCGATGTGATTCTGCTGGGAGAATTGCGCGATACGGAGACGATTCGGC
TGGCGCTGACAGCGGCGGAAACCGGCCATCTGGTGCTATCCACGCTGCATACGCGCAGTGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCCGGTGAAGAGAAAGCCTATGTTCGCAGTCAGCTATCCACCTGCTTGCAGGCCGTGGTGACGCA
AAGGTTGTTGCCTTCTGCACACGGCGGTCGTATTGCGCTTTATGAAGTGCTGACGGCGACAGCGGCAGTCAGCAACCTGA
TTCGGGAAGGGAAAACGCATCAGCTTCCCGGCCTGATTCAAACGGGAACGGCTGCGGGCATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAATTGCGCCCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

50

99.408

0.497
  pilT Vibrio cholerae strain A1552

50.909

97.633

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.909

97.633

0.497
  pilT Neisseria gonorrhoeae MS11

49.702

99.408

0.494
  pilT Pseudomonas stutzeri DSM 10701

49.254

99.112

0.488
  pilT Acinetobacter baylyi ADP1

48.521

100

0.485
  pilT Legionella pneumophila strain ERS1305867

48.955

99.112

0.485
  pilT Pseudomonas aeruginosa PAK

48.955

99.112

0.485
  pilT Legionella pneumophila strain Lp02

48.955

99.112

0.485
  pilT Acinetobacter nosocomialis M2

48.06

99.112

0.476
  pilT Acinetobacter baumannii D1279779

48.06

99.112

0.476
  pilT Acinetobacter baumannii strain A118

48.06

99.112

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.246

99.704

0.411
  pilU Pseudomonas stutzeri DSM 10701

40.95

99.704

0.408
  pilU Vibrio cholerae strain A1552

39.466

99.704

0.393
  pilU Acinetobacter baylyi ADP1

39.441

95.266

0.376