Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L0Y14_RS00360 Genome accession   NZ_CP090569
Coordinates   78356..79465 (-) Length   369 a.a.
NCBI ID   WP_040819744.1    Uniprot ID   A0A9J6ZY92
Organism   Candidatus Endoriftia persephone isolate Tica-EPR-9o50.N     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 73356..84465
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0Y14_RS00345 (L0Y14_00345) lysA 73949..75196 (-) 1248 WP_006475166.1 diaminopimelate decarboxylase -
  L0Y14_RS16570 lptM 75200..75376 (-) 177 WP_081417766.1 LPS translocon maturation chaperone LptM -
  L0Y14_RS00350 (L0Y14_00350) - 75441..75725 (-) 285 WP_005965486.1 YcgL domain-containing protein -
  L0Y14_RS00355 (L0Y14_00355) - 75722..78250 (-) 2529 WP_006475167.1 glycogen/starch/alpha-glucan phosphorylase -
  L0Y14_RS00360 (L0Y14_00360) pilU 78356..79465 (-) 1110 WP_040819744.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L0Y14_RS00365 (L0Y14_00365) - 79580..80398 (+) 819 WP_005965491.1 MOSC domain-containing protein -
  L0Y14_RS00370 (L0Y14_00370) - 80391..80951 (+) 561 WP_006475169.1 DUF1415 domain-containing protein -
  L0Y14_RS00375 (L0Y14_00375) - 80948..81418 (-) 471 WP_005965494.1 DUF2939 domain-containing protein -
  L0Y14_RS00380 (L0Y14_00380) - 81497..81772 (-) 276 WP_006475170.1 RNA recognition motif domain-containing protein -
  L0Y14_RS00385 (L0Y14_00385) - 81908..83818 (-) 1911 WP_006475171.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41327.54 Da        Isoelectric Point: 6.7705

>NTDB_id=643528 L0Y14_RS00360 WP_040819744.1 78356..79465(-) (pilU) [Candidatus Endoriftia persephone isolate Tica-EPR-9o50.N]
MDIVPYLKLMVQKNGSDLFFSTGAPPNLKAEGDCRPIGSTPLQPGQVRKLAYSVMSDDQIHEFEATLECNMAISISKLGR
FRVNVFRQRGDVAMVIRYIKGVIPPVEQLNLPVILKDLILQKHGLILVVGATGSGKSTTLAAMLEHRNQNMYGHILTIED
PIEYLYTHKRSIVDQREVGIDTLSYENALKNAMREAPDVIQIGEIRDMETMKYAIAYAETGHLCLSTLHSNNANQALDRI
INFFPDTARQQLYMDLSLNLKAVISQRLVRNVNGQRLPAVEILLLTSYISELIQKGDIHGIKEAMEQGTERGMQTFDQSL
YKLYREERISLEEALSHADSRNNLSLKIRLSEGASPGFSGNLGITEKQP

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=643528 L0Y14_RS00360 WP_040819744.1 78356..79465(-) (pilU) [Candidatus Endoriftia persephone isolate Tica-EPR-9o50.N]
ATGGATATCGTCCCTTACTTGAAACTCATGGTGCAGAAAAACGGCTCGGATCTCTTCTTCAGTACTGGCGCACCACCCAA
CCTCAAGGCCGAGGGGGATTGCCGTCCGATCGGCTCCACCCCGCTGCAGCCCGGCCAGGTGCGCAAGCTCGCCTACTCGG
TGATGTCAGACGATCAGATACACGAGTTCGAGGCGACGCTGGAGTGCAATATGGCCATTTCCATCAGCAAACTGGGGCGG
TTTCGCGTCAATGTCTTCAGACAACGCGGCGATGTGGCGATGGTGATCCGCTACATCAAAGGGGTGATCCCGCCGGTGGA
ACAGCTCAATCTGCCGGTCATCCTGAAGGATCTGATCCTGCAGAAGCACGGCCTGATCCTGGTGGTCGGCGCCACCGGTT
CGGGCAAGTCGACCACCCTGGCGGCGATGCTCGAGCACCGCAACCAGAACATGTATGGCCACATTCTCACCATCGAGGAT
CCGATCGAATACCTCTACACCCACAAGCGGTCGATCGTCGACCAGCGCGAGGTTGGCATAGATACTCTCAGCTATGAAAA
TGCCCTAAAGAACGCCATGCGCGAGGCCCCTGACGTGATCCAGATCGGCGAGATCCGCGACATGGAGACGATGAAATACG
CCATCGCCTACGCCGAGACTGGCCACCTCTGCCTCTCCACCCTGCACTCCAACAACGCCAATCAGGCCCTCGACCGGATC
ATCAACTTCTTTCCCGACACCGCCCGGCAGCAGCTCTACATGGACCTCTCACTCAACCTCAAGGCGGTGATCTCACAACG
CCTGGTGCGCAACGTCAACGGCCAGCGCCTGCCGGCAGTAGAGATCTTGCTACTCACCAGCTACATCTCTGAGCTGATCC
AGAAGGGCGACATCCACGGCATCAAAGAGGCGATGGAACAGGGCACAGAACGCGGCATGCAGACCTTCGACCAGTCGCTC
TATAAGCTCTACCGGGAGGAGCGGATCAGCCTGGAGGAGGCCCTCAGCCACGCCGACTCACGCAACAACCTATCGCTGAA
GATCCGCCTCTCGGAAGGCGCCAGCCCTGGCTTCTCCGGCAATCTCGGCATCACTGAGAAGCAGCCCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.416

95.122

0.518
  pilU Acinetobacter baylyi ADP1

53.221

96.748

0.515
  pilU Vibrio cholerae strain A1552

50.413

98.374

0.496
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.382

97.832

0.415
  pilT Legionella pneumophila strain Lp02

43.114

90.515

0.39
  pilT Legionella pneumophila strain ERS1305867

43.114

90.515

0.39
  pilT Acinetobacter baylyi ADP1

41.765

92.141

0.385
  pilT Pseudomonas aeruginosa PAK

40.988

93.225

0.382
  pilT Vibrio cholerae strain A1552

41.369

91.057

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.369

91.057

0.377
  pilT Pseudomonas stutzeri DSM 10701

40.116

93.225

0.374
  pilT Acinetobacter baumannii D1279779

40.828

91.599

0.374
  pilT Acinetobacter nosocomialis M2

40.828

91.599

0.374
  pilT Acinetobacter baumannii strain A118

40.828

91.599

0.374
  pilT Neisseria gonorrhoeae MS11

39.193

94.038

0.369
  pilT Neisseria meningitidis 8013

39.193

94.038

0.369