Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IHC87_RS02275 Genome accession   NZ_CP090501
Coordinates   449368..450402 (+) Length   344 a.a.
NCBI ID   WP_005297689.1    Uniprot ID   -
Organism   Photobacterium damselae subsp. damselae strain CSP DAM1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 444368..455402
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHC87_RS02240 (IHC87_02240) - 444611..445015 (+) 405 WP_050787742.1 DUF2884 family protein -
  IHC87_RS02245 (IHC87_02245) hemW 445090..446250 (-) 1161 WP_237033364.1 radical SAM family heme chaperone HemW -
  IHC87_RS02250 (IHC87_02250) - 446250..446843 (-) 594 WP_237033365.1 XTP/dITP diphosphatase -
  IHC87_RS02255 (IHC87_02255) yggU 446945..447244 (-) 300 WP_036763214.1 DUF167 family protein YggU -
  IHC87_RS02260 (IHC87_02260) - 447241..447798 (-) 558 WP_005297682.1 YggT family protein -
  IHC87_RS02265 (IHC87_02265) proC 447810..448628 (-) 819 WP_005297684.1 pyrroline-5-carboxylate reductase -
  IHC87_RS02270 (IHC87_02270) - 448644..449345 (-) 702 WP_005297686.1 YggS family pyridoxal phosphate-dependent enzyme -
  IHC87_RS02275 (IHC87_02275) pilT 449368..450402 (+) 1035 WP_005297689.1 type IV pilus twitching motility protein PilT Machinery gene
  IHC87_RS02280 (IHC87_02280) pilU 450414..451520 (+) 1107 WP_005297691.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IHC87_RS02285 (IHC87_02285) ruvX 451848..452273 (-) 426 WP_005297693.1 Holliday junction resolvase RuvX -
  IHC87_RS02290 (IHC87_02290) - 452270..452833 (-) 564 WP_068968286.1 YqgE/AlgH family protein -
  IHC87_RS02295 (IHC87_02295) gshB 452987..453934 (-) 948 WP_005297697.1 glutathione synthase -
  IHC87_RS02300 (IHC87_02300) rsmE 453955..454686 (-) 732 WP_044174434.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38180.73 Da        Isoelectric Point: 6.1433

>NTDB_id=642972 IHC87_RS02275 WP_005297689.1 449368..450402(+) (pilT) [Photobacterium damselae subsp. damselae strain CSP DAM1]
MDITELLDFSVKHNASDLHLSAGVPPMIRVDGDVRKLSLPALEHSEVHRLIFDIMNDAQRRQFEEELEVDFSFELPDVGR
FRVNAFHQSRGCSAVFRTIPMTIPTLESLNVPDVFYKIAQCQRGLVLVTGATGSGKSTTIAALVDYINEHTNRHILTIED
PIEFVHHSKRCLINQREVHRDTHSFQAALRSALREDPDVIVVGELRDQETISLALTAAETGHLVLGTLHTSSAAKSIDRI
IDVFPGSDKSMVRSMLSESLRAVVSQSLLKCISGGRVASHEIMMATPAIRNLIREDKIAQMYSMIQTGSAMGMQTMEQSV
KMLVAQGLVEAEEGRRIVDSAQQI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=642972 IHC87_RS02275 WP_005297689.1 449368..450402(+) (pilT) [Photobacterium damselae subsp. damselae strain CSP DAM1]
ATGGATATCACCGAATTATTAGACTTCAGTGTAAAACATAACGCATCGGATCTGCATCTTTCAGCAGGTGTTCCACCTAT
GATCCGTGTAGATGGTGATGTACGCAAGTTAAGTTTACCTGCATTAGAGCACTCAGAGGTTCATCGTCTGATTTTTGATA
TCATGAATGATGCCCAACGTCGTCAATTTGAAGAAGAGTTAGAAGTCGACTTCTCATTTGAATTACCCGATGTTGGCCGT
TTTCGTGTTAACGCCTTTCACCAATCTCGAGGTTGTTCTGCGGTCTTTCGTACTATCCCTATGACCATTCCAACCTTGGA
ATCTCTGAATGTGCCCGATGTTTTCTACAAAATAGCACAATGTCAGCGTGGATTGGTATTAGTTACAGGGGCTACAGGTT
CTGGTAAATCCACCACCATTGCGGCATTGGTTGATTATATTAATGAGCATACCAATCGTCATATCCTCACGATTGAAGAT
CCAATTGAATTTGTGCATCACAGTAAACGTTGTTTGATTAACCAACGAGAAGTGCATCGTGATACCCACAGTTTTCAAGC
CGCTTTGCGCTCGGCATTACGTGAAGATCCCGATGTGATTGTTGTGGGGGAATTGCGTGATCAAGAGACCATCAGTTTAG
CGTTAACTGCTGCAGAAACCGGACACTTGGTTTTAGGTACGCTACACACAAGTTCCGCAGCCAAAAGTATTGACCGTATT
ATCGATGTATTTCCTGGTAGTGATAAATCCATGGTTCGCTCCATGTTATCTGAGTCATTACGTGCAGTGGTTTCCCAAAG
TTTGTTGAAATGCATTAGCGGTGGTCGTGTGGCTTCCCATGAGATCATGATGGCAACCCCTGCGATTCGAAATTTGATCC
GCGAAGATAAAATTGCTCAGATGTATTCCATGATCCAAACCGGATCGGCAATGGGAATGCAGACAATGGAACAGAGTGTG
AAGATGCTGGTTGCTCAAGGACTAGTGGAAGCAGAAGAAGGTCGTCGTATAGTCGACAGTGCCCAACAGATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

79.88

96.802

0.773
  pilT Vibrio cholerae strain A1552

79.88

96.802

0.773
  pilT Acinetobacter baumannii D1279779

70.448

97.384

0.686
  pilT Acinetobacter baumannii strain A118

70.448

97.384

0.686
  pilT Acinetobacter nosocomialis M2

70.149

97.384

0.683
  pilT Pseudomonas aeruginosa PAK

70.149

97.384

0.683
  pilT Pseudomonas stutzeri DSM 10701

69.851

97.384

0.68
  pilT Acinetobacter baylyi ADP1

68.421

99.419

0.68
  pilT Legionella pneumophila strain Lp02

69.605

95.64

0.666
  pilT Legionella pneumophila strain ERS1305867

69.605

95.64

0.666
  pilT Neisseria meningitidis 8013

65.579

97.965

0.642
  pilT Neisseria gonorrhoeae MS11

65.282

97.965

0.64
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

100

0.5
  pilU Vibrio cholerae strain A1552

41.159

100

0.413
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

39.13

93.605

0.366