Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LZP85_RS16110 Genome accession   NZ_CP090431
Coordinates   3118167..3119207 (-) Length   346 a.a.
NCBI ID   WP_206782145.1    Uniprot ID   A0A8I1MCD7
Organism   Priestia flexa strain DMP08     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3113167..3124207
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LZP85_RS16085 (LZP85_16085) - 3113821..3114447 (-) 627 WP_142925374.1 hypothetical protein -
  LZP85_RS16090 (LZP85_16090) pilM 3114444..3115436 (-) 993 WP_234561796.1 type IV pilus biogenesis protein PilM -
  LZP85_RS16095 (LZP85_16095) - 3115450..3116202 (-) 753 WP_234561798.1 A24 family peptidase -
  LZP85_RS16100 (LZP85_16100) - 3116202..3116615 (-) 414 WP_234561800.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LZP85_RS16105 (LZP85_16105) - 3116953..3118167 (-) 1215 WP_076512665.1 type II secretion system F family protein -
  LZP85_RS16110 (LZP85_16110) pilT 3118167..3119207 (-) 1041 WP_206782145.1 type IV pilus twitching motility protein PilT Machinery gene
  LZP85_RS16115 (LZP85_16115) - 3119219..3120886 (-) 1668 WP_206833172.1 GspE/PulE family protein -
  LZP85_RS16120 (LZP85_16120) - 3120899..3122338 (-) 1440 WP_206782147.1 VanW family protein -
  LZP85_RS16125 (LZP85_16125) - 3122355..3123140 (-) 786 WP_076512661.1 PRC-barrel domain-containing protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38247.00 Da        Isoelectric Point: 7.6293

>NTDB_id=642381 LZP85_RS16110 WP_206782145.1 3118167..3119207(-) (pilT) [Priestia flexa strain DMP08]
MRSKVEHLMRSAFQLKASDVHLTAGVPPIFRINGALKRYGKENLVPEELEEMAKNIIDPIRWGDFKEKGELDFSYAVEGL
SRFRVNAYHQRGSISIAIRIVPTKIPSLEDLKLPEVLKTITTKSQGLVLVTGPTGSGKSTTLASMIDFMNRTASRHIITL
EDPIEYLHRHGSCIIDQREVGFDTHSFANGLRASLRQDPDVILVGELRDLETIHTAITAAETGHLVLATLHTSSAPATIN
RIIDVFPAAQQEQIRVQLSSVLVSVISQRLLPTTDLQNRTCATEILMNNPAVSNLIRSEKIHQIPNVMQTSRSLGMHTLA
QSVNELLQAGVITKKVAEPYVNEAVS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=642381 LZP85_RS16110 WP_206782145.1 3118167..3119207(-) (pilT) [Priestia flexa strain DMP08]
ATGAGAAGCAAAGTGGAACATTTAATGAGAAGTGCATTCCAGCTAAAGGCGTCAGACGTACATTTAACTGCAGGCGTCCC
GCCGATTTTTCGCATCAACGGTGCTTTAAAGCGCTATGGAAAAGAAAATTTAGTTCCAGAAGAGCTTGAAGAGATGGCAA
AGAATATTATTGATCCAATAAGATGGGGAGACTTCAAAGAAAAAGGCGAGCTCGATTTTTCATACGCTGTGGAAGGATTA
TCGCGCTTTCGCGTCAACGCTTATCATCAGCGCGGTTCAATTTCAATTGCCATTCGAATTGTGCCTACGAAGATTCCGAG
TTTAGAAGATTTAAAGCTTCCGGAAGTATTAAAAACAATTACGACGAAATCTCAGGGACTTGTTCTTGTGACTGGACCAA
CGGGAAGTGGGAAGTCAACAACGCTTGCGTCTATGATCGATTTTATGAATCGTACGGCAAGTCGTCATATTATTACGCTA
GAAGATCCGATTGAGTACTTACATCGCCACGGTAGCTGCATTATTGATCAGCGGGAAGTGGGGTTTGATACACACAGCTT
TGCCAACGGTCTGCGCGCTTCGCTTCGTCAGGATCCTGACGTTATTCTAGTGGGAGAGCTTCGCGACCTAGAAACGATTC
ATACTGCCATTACGGCAGCTGAAACCGGACACTTAGTGCTCGCTACGCTGCATACGTCAAGCGCACCGGCAACGATTAAC
CGTATTATTGACGTATTCCCAGCTGCGCAGCAGGAGCAAATCCGCGTCCAGCTCTCTTCGGTGCTCGTGAGCGTCATTTC
ACAGCGTTTGCTGCCAACGACGGACTTGCAAAATCGCACGTGCGCAACGGAAATTTTAATGAATAATCCAGCTGTATCCA
ACCTGATTCGCAGTGAAAAAATTCATCAAATTCCAAACGTTATGCAAACAAGTCGTTCTCTTGGCATGCATACACTCGCT
CAGTCGGTCAATGAGCTTTTGCAAGCGGGTGTGATTACGAAAAAAGTAGCGGAACCGTATGTCAACGAGGCGGTTAGCTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.368

95.087

0.488
  pilT Legionella pneumophila strain Lp02

47.246

99.711

0.471
  pilT Legionella pneumophila strain ERS1305867

47.246

99.711

0.471
  pilT Acinetobacter nosocomialis M2

48.171

94.798

0.457
  pilT Acinetobacter baumannii D1279779

48.171

94.798

0.457
  pilT Acinetobacter baumannii strain A118

48.171

94.798

0.457
  pilT Neisseria meningitidis 8013

48.607

93.353

0.454
  pilT Vibrio cholerae strain A1552

47.866

94.798

0.454
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.866

94.798

0.454
  pilT Pseudomonas stutzeri DSM 10701

47.72

95.087

0.454
  pilT Pseudomonas aeruginosa PAK

47.561

94.798

0.451
  pilT Acinetobacter baylyi ADP1

47.256

94.798

0.448
  pilT Neisseria gonorrhoeae MS11

47.516

93.064

0.442
  pilU Pseudomonas stutzeri DSM 10701

43.114

96.532

0.416
  pilU Acinetobacter baylyi ADP1

45.513

90.173

0.41
  pilU Vibrio cholerae strain A1552

42.415

93.353

0.396