Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LW347_RS17485 Genome accession   NZ_CP090065
Coordinates   3868781..3869797 (-) Length   338 a.a.
NCBI ID   WP_258882994.1    Uniprot ID   A0AAE9NM80
Organism   Pectobacterium polonicum strain BY21311     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3863781..3874797
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LW347_RS17470 (LW347_17450) nsrR 3864176..3864601 (-) 426 WP_258882991.1 nitric oxide-sensing transcriptional repressor NsrR -
  LW347_RS17475 (LW347_17455) - 3864896..3866467 (+) 1572 WP_258882992.1 methyl-accepting chemotaxis protein -
  LW347_RS17480 (LW347_17460) - 3866980..3868812 (+) 1833 WP_258882993.1 diguanylate cyclase -
  LW347_RS17485 (LW347_17465) pilT 3868781..3869797 (-) 1017 WP_258882994.1 type IV pilus twitching motility protein PilT Machinery gene
  LW347_RS17490 (LW347_17470) - 3869819..3870532 (+) 714 WP_258882995.1 YggS family pyridoxal phosphate-dependent enzyme -
  LW347_RS17495 (LW347_17475) proC 3870619..3871440 (+) 822 WP_258882996.1 pyrroline-5-carboxylate reductase -
  LW347_RS17500 (LW347_17480) - 3871490..3872044 (+) 555 WP_137741245.1 YggT family protein -
  LW347_RS17505 (LW347_17485) yggU 3872041..3872331 (+) 291 WP_010295005.1 DUF167 family protein YggU -
  LW347_RS17510 (LW347_17490) - 3872363..3872956 (+) 594 WP_258882997.1 XTP/dITP diphosphatase -
  LW347_RS17515 (LW347_17495) hemW 3872949..3874091 (+) 1143 WP_258882998.1 radical SAM family heme chaperone HemW -
  LW347_RS17520 (LW347_17500) - 3874320..3874592 (+) 273 WP_258882999.1 type II toxin-antitoxin system ParD family antitoxin -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36999.38 Da        Isoelectric Point: 6.2762

>NTDB_id=640505 LW347_RS17485 WP_258882994.1 3868781..3869797(-) (pilT) [Pectobacterium polonicum strain BY21311]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPCLMQEQVAQWCDVWLEPTQKEQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSVALRIIPFVQPSLDELHAPPVLATLLEKPNGLILITGATGSGKSTTLAAMVGTLNDSSDRHVITLE
DPIEFIHTSRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQKLLPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGTAVGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=640505 LW347_RS17485 WP_258882994.1 3868781..3869797(-) (pilT) [Pectobacterium polonicum strain BY21311]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGGCATCCGCCGGT
ATTACGTGTGGATGGGCGGCTACAGCCTGAAAATACCTTACCATGTTTAATGCAGGAACAGGTGGCACAATGGTGTGATG
TCTGGCTAGAGCCGACTCAGAAAGAACAATTGCGACAGGCTGGGCAGGTGGATGGCGCGTTAATGCTGCCTGATGGTCAG
CGCCTGCGGGTAAATGTGTTTCGTCAACGGGAAGGGTTATCTGTCGCGCTGCGTATTATCCCGTTTGTGCAACCTTCATT
GGATGAGCTACATGCGCCACCCGTCCTAGCGACACTGCTGGAGAAACCAAACGGGCTGATTCTGATTACCGGTGCGACGG
GCAGCGGTAAATCCACGACGCTGGCGGCAATGGTTGGAACGTTAAACGACAGCAGCGATCGCCATGTGATCACGCTGGAA
GATCCGATCGAGTTTATTCATACCAGCCGGCGCTGTCTGATCCAACAGCGAGAGATCGGTGCACACAGCGCGTCTTTTGC
TCAGGCGCTACGAGCGGCATTGCGGGAAGATCCCGATGTTATTCTACTGGGAGAACTGCGCGATACGGAGACGATTCGTC
TGGCGTTGACGGCGGCGGAAACCGGCCATTTGGTGCTATCAACGCTACATACACGCAGTGCATCGCAGGCCGTTGATCGG
CTAATTGATGTCTTCCCCGGTGAAGAGAAAGCGTATGTTCGCAGCCAGTTAGCGACCTGTTTGCAGGCTGTAGTGACGCA
AAAGCTGTTGCCCGCCGCGCAAGGTGGCCGAATCGCCCTTTATGAAGTGCTGACGGCGACAGCAGCAGTCAGCAACTTGA
TTCGTGAAGGGAAAACGCACCAGCTTCCCGGCTTGATTCAGACGGGAACCGCAGTGGGAATGCAAACGTTCGAACAAAGC
TATCAGCAGCGCTGTCGGGACGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

50

98.225

0.491
  pilT Vibrio cholerae strain A1552

50.303

97.633

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.303

97.633

0.491
  pilT Neisseria gonorrhoeae MS11

49.699

98.225

0.488
  pilT Pseudomonas aeruginosa PAK

48.036

97.929

0.47
  pilT Legionella pneumophila strain Lp02

48.036

97.929

0.47
  pilT Legionella pneumophila strain ERS1305867

48.036

97.929

0.47
  pilT Pseudomonas stutzeri DSM 10701

48.036

97.929

0.47
  pilT Acinetobacter baylyi ADP1

47.734

97.929

0.467
  pilT Acinetobacter nosocomialis M2

47.13

97.929

0.462
  pilT Acinetobacter baumannii D1279779

47.13

97.929

0.462
  pilT Acinetobacter baumannii strain A118

47.13

97.929

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.136

99.704

0.42
  pilU Vibrio cholerae strain A1552

38.776

100

0.393
  pilU Pseudomonas stutzeri DSM 10701

39.94

98.521

0.393
  pilU Acinetobacter baylyi ADP1

38.509

95.266

0.367