Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LS684_RS15495 Genome accession   NZ_CP089997
Coordinates   3050359..3051399 (-) Length   346 a.a.
NCBI ID   WP_233807257.1    Uniprot ID   -
Organism   Cytobacillus spongiae strain CY-G     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3045359..3056399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LS684_RS15470 (LS684_15470) - 3046103..3046741 (-) 639 WP_233807253.1 fimbrial protein -
  LS684_RS15475 (LS684_15475) pilM 3046744..3047736 (-) 993 WP_233807254.1 pilus assembly protein PilM -
  LS684_RS15480 (LS684_15480) - 3047755..3048507 (-) 753 WP_233811678.1 A24 family peptidase -
  LS684_RS15485 (LS684_15485) - 3048528..3049037 (-) 510 WP_233807255.1 type II secretion system protein -
  LS684_RS15490 (LS684_15490) - 3049145..3050359 (-) 1215 WP_233807256.1 type II secretion system F family protein -
  LS684_RS15495 (LS684_15495) pilT 3050359..3051399 (-) 1041 WP_233807257.1 type IV pilus twitching motility protein PilT Machinery gene
  LS684_RS15500 (LS684_15500) - 3051411..3053072 (-) 1662 WP_233807258.1 ATPase, T2SS/T4P/T4SS family -
  LS684_RS15505 (LS684_15505) - 3053099..3054469 (-) 1371 WP_233807260.1 VanW family protein -
  LS684_RS15510 (LS684_15510) - 3054490..3055251 (-) 762 WP_233807261.1 PRC-barrel domain-containing protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38536.53 Da        Isoelectric Point: 8.7432

>NTDB_id=639920 LS684_RS15495 WP_233807257.1 3050359..3051399(-) (pilT) [Cytobacillus spongiae strain CY-G]
MKEKIDYLLRAGYELKASDIHLTVGVPPIMRMNGDLKKYGKDTLKPEDTEGMAKAIIPENMWKQFKEKGELDFSYGIPGI
SRFRVNTYFQRSCVAMAVRVVPTKIPTIEELDLPNILTKIAEKPQGLVLVTGPTGSGKSTTLASMIQYMNHHMRKHIITL
EDPIEYLHKHGNSIIDQREVGFDTNNFANGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHLVFGTLHTSSAPATIN
RIIDVFPPAQQAQIRIQLASVLVSIVSQRLFPTVDKQGRKAATEILINNAAVANLIRNEKIHQIVNIMQTSRAQGMHTLE
SNIKELVNRGSISNEVAAPFLQEMHN

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=639920 LS684_RS15495 WP_233807257.1 3050359..3051399(-) (pilT) [Cytobacillus spongiae strain CY-G]
ATGAAAGAAAAAATCGACTATTTACTACGCGCCGGATATGAGCTGAAAGCATCTGATATTCATTTAACAGTTGGGGTCCC
ACCGATCATGCGAATGAATGGCGATTTAAAAAAGTATGGAAAAGATACTTTAAAGCCTGAAGATACAGAAGGTATGGCGA
AAGCGATCATTCCTGAAAATATGTGGAAGCAGTTTAAAGAAAAAGGGGAGCTCGACTTTTCTTATGGTATCCCAGGGATA
TCTCGCTTTCGTGTAAACACATACTTCCAACGTTCATGTGTGGCAATGGCAGTACGTGTCGTTCCGACGAAAATCCCTAC
TATAGAAGAGCTTGACCTTCCAAATATATTAACGAAAATTGCCGAAAAGCCACAAGGACTTGTCCTTGTAACCGGCCCAA
CAGGTAGTGGTAAATCTACGACACTTGCTTCAATGATACAGTATATGAATCATCATATGCGCAAGCATATTATTACGTTA
GAAGACCCAATTGAATATTTACACAAACATGGAAATTCGATTATTGACCAGCGCGAAGTAGGTTTTGATACGAATAATTT
TGCTAATGGACTTCGAGCTGCGTTAAGACAGGACCCAGATGTCATTCTAGTTGGAGAGATGAGAGATTTAGAGACTATAC
AAACAGCGATTACAGCTGCAGAAACGGGCCATTTAGTTTTTGGAACACTACATACGTCTAGTGCTCCGGCAACGATCAAC
CGAATAATTGATGTTTTTCCTCCGGCACAGCAGGCACAAATTCGAATTCAGCTTGCCTCTGTGTTAGTATCAATCGTGTC
GCAGCGCCTTTTTCCTACTGTTGATAAACAAGGCAGAAAGGCAGCAACGGAAATTCTGATTAATAATGCGGCTGTAGCTA
ACCTTATTCGCAATGAGAAAATTCATCAAATCGTTAATATTATGCAAACATCTCGTGCGCAAGGGATGCATACGTTAGAG
AGTAATATTAAGGAGCTCGTTAATAGAGGATCCATATCAAATGAGGTCGCGGCTCCATTCTTACAGGAGATGCATAACTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.266

95.665

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.76

95.087

0.483
  pilT Vibrio cholerae strain A1552

50.76

95.087

0.483
  pilT Neisseria meningitidis 8013

48.802

96.532

0.471
  pilT Neisseria gonorrhoeae MS11

48.204

96.532

0.465
  pilT Legionella pneumophila strain Lp02

48.048

96.243

0.462
  pilT Legionella pneumophila strain ERS1305867

48.048

96.243

0.462
  pilT Pseudomonas aeruginosa PAK

47.147

96.243

0.454
  pilT Pseudomonas stutzeri DSM 10701

46.547

96.243

0.448
  pilT Acinetobacter baylyi ADP1

46.505

95.087

0.442
  pilT Acinetobacter baumannii D1279779

47.664

92.775

0.442
  pilT Acinetobacter baumannii strain A118

47.664

92.775

0.442
  pilT Acinetobacter nosocomialis M2

47.664

92.775

0.442
  pilU Pseudomonas stutzeri DSM 10701

43.413

96.532

0.419
  pilU Acinetobacter baylyi ADP1

41.693

92.197

0.384
  pilU Vibrio cholerae strain A1552

40.062

93.064

0.373