Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LVW35_RS26940 Genome accession   NZ_CP089985
Coordinates   5862813..5863847 (-) Length   344 a.a.
NCBI ID   WP_233892762.1    Uniprot ID   -
Organism   Pseudomonas sp. HN11     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5857813..5868847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVW35_RS26910 (LVW35_26910) ruvX 5857974..5858411 (+) 438 WP_005792259.1 Holliday junction resolvase RuvX -
  LVW35_RS26915 (LVW35_26915) pyrR 5858485..5858991 (+) 507 WP_233892756.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  LVW35_RS26920 (LVW35_26920) - 5859016..5860020 (+) 1005 WP_233892757.1 aspartate carbamoyltransferase catalytic subunit -
  LVW35_RS26925 (LVW35_26925) - 5860017..5861288 (+) 1272 WP_233892758.1 dihydroorotase -
  LVW35_RS26930 (LVW35_26930) - 5861358..5861768 (-) 411 WP_164393875.1 TM2 domain-containing protein -
  LVW35_RS26935 (LVW35_26935) - 5862036..5862674 (+) 639 WP_233892760.1 C40 family peptidase -
  LVW35_RS26940 (LVW35_26940) pilT 5862813..5863847 (-) 1035 WP_233892762.1 type IV pilus twitching motility protein PilT Machinery gene
  LVW35_RS26945 (LVW35_26945) - 5863905..5864591 (+) 687 WP_233892763.1 YggS family pyridoxal phosphate-dependent enzyme -
  LVW35_RS26950 (LVW35_26950) proC 5864625..5865443 (+) 819 WP_233892764.1 pyrroline-5-carboxylate reductase -
  LVW35_RS26955 (LVW35_26955) - 5865453..5866043 (+) 591 WP_185709128.1 YggT family protein -
  LVW35_RS26960 (LVW35_26960) - 5866139..5867278 (+) 1140 WP_233892765.1 homoserine O-acetyltransferase -
  LVW35_RS26965 (LVW35_26965) metW 5867286..5867906 (+) 621 WP_010207236.1 methionine biosynthesis protein MetW -
  LVW35_RS26970 (LVW35_26970) - 5867928..5868362 (+) 435 WP_233892766.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37563.35 Da        Isoelectric Point: 6.6695

>NTDB_id=639715 LVW35_RS26940 WP_233892762.1 5862813..5863847(-) (pilT) [Pseudomonas sp. HN11]
MDISELLTVSVRRGASDLHLSAGLTPMLRVDGEVWPLEGPVLSPTQVADLLSPLLNQHQQKDFETSLETDFAFELPGVAR
FRANVFRQDRGMSAVFRTIPSEVQSLENLGLDEVFRRIAQLPRGLVLVTGPTGSGKSTTLAAMIDFLNQHRRQHILTLED
PIEFIHTPKMALINQRQVHRDTHGFSIALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRL
VDVFPAGEKAMVRSMLSESLQAVVSQVLVKKIGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGALGMKTLDMSL
KALVGEGVISREDAREKARVPADI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=639715 LVW35_RS26940 WP_233892762.1 5862813..5863847(-) (pilT) [Pseudomonas sp. HN11]
ATGGATATCTCAGAATTACTCACGGTCAGCGTGCGCCGTGGCGCCTCCGACCTGCATTTGTCCGCCGGCCTGACGCCGAT
GCTGCGAGTGGATGGCGAGGTCTGGCCACTGGAGGGGCCAGTGCTTTCACCTACGCAAGTAGCGGACTTATTGAGCCCTT
TGCTCAATCAGCACCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCCTTCGAACTGCCCGGAGTGGCGCGG
TTCCGGGCCAATGTGTTTCGTCAGGATCGCGGCATGAGTGCAGTGTTTCGCACCATCCCGTCCGAAGTCCAAAGCCTGGA
AAACCTTGGCCTTGATGAAGTGTTCCGGCGTATCGCTCAACTGCCACGGGGTTTGGTGTTGGTCACCGGCCCGACCGGTT
CAGGCAAGTCCACCACCCTGGCGGCGATGATCGATTTTCTCAATCAGCATCGGCGCCAGCACATTCTCACCCTTGAAGAC
CCCATCGAATTTATCCACACGCCGAAAATGGCGTTGATCAACCAACGCCAGGTGCATCGCGACACCCATGGTTTTTCCAT
CGCCCTGCGCTCGGCGCTTCGGGAAGACCCGGACGTGATCCTGGTGGGCGAACTGCGTGACCTGGAAACCATCCGTCTGG
CGCTGACGGCGGCTGAGACCGGGCACCTGGTGTTTGGCACCCTGCACACCAGTTCGGCGGCCAAGACTGTCGACCGACTG
GTGGACGTGTTCCCCGCCGGGGAAAAGGCGATGGTCCGCTCGATGCTGTCGGAGTCGTTGCAGGCGGTGGTGTCCCAAGT
GCTGGTGAAGAAGATTGGCGGCGGGCGGGTAGCGGCCCATGAAATCATGCTGGGCACGCCGGCCATTCGGAATTTGATTC
GGGAGGACAAGGTGGCGCAGATGGTCTCGGCGATCCAGACCGGCGGGGCGTTGGGGATGAAGACCCTGGATATGAGTTTG
AAGGCGCTGGTCGGGGAGGGGGTGATCAGTCGGGAGGATGCGCGGGAGAAGGCGAGGGTGCCTGCAGACATATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(79-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

73.256

100

0.733
  pilT Pseudomonas stutzeri DSM 10701

71.221

100

0.712
  pilT Acinetobacter baumannii D1279779

69.795

99.128

0.692
  pilT Acinetobacter baumannii strain A118

69.795

99.128

0.692
  pilT Acinetobacter nosocomialis M2

69.795

99.128

0.692
  pilT Acinetobacter baylyi ADP1

69.208

99.128

0.686
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.688

97.965

0.634
  pilT Vibrio cholerae strain A1552

64.688

97.965

0.634
  pilT Legionella pneumophila strain Lp02

64.201

98.256

0.631
  pilT Legionella pneumophila strain ERS1305867

64.201

98.256

0.631
  pilT Neisseria meningitidis 8013

61.471

98.837

0.608
  pilT Neisseria gonorrhoeae MS11

61.176

98.837

0.605
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.406

100

0.485
  pilU Vibrio cholerae strain A1552

38.841

100

0.39
  pilU Acinetobacter baylyi ADP1

38.439

100

0.387
  pilU Pseudomonas stutzeri DSM 10701

39.403

97.384

0.384