Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LU301_RS04770 Genome accession   NZ_CP089978
Coordinates   935600..936637 (-) Length   345 a.a.
NCBI ID   WP_305273216.1    Uniprot ID   -
Organism   Moraxella sp. ZY210820     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 930600..941637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LU301_RS04750 (LU301_04740) - 931269..932642 (-) 1374 WP_305273204.1 wax ester/triacylglycerol synthase family O-acyltransferase -
  LU301_RS04755 (LU301_04745) - 932733..933092 (-) 360 WP_305273207.1 hypothetical protein -
  LU301_RS04760 (LU301_04750) hemB 933158..934168 (+) 1011 WP_305273210.1 porphobilinogen synthase -
  LU301_RS04765 (LU301_04755) - 934323..935501 (+) 1179 WP_305273213.1 MBL fold metallo-hydrolase -
  LU301_RS04770 (LU301_04760) pilT 935600..936637 (-) 1038 WP_305273216.1 type IV pilus twitching motility protein PilT Machinery gene
  LU301_RS04775 (LU301_04765) - 936867..937553 (+) 687 WP_305273217.1 YggS family pyridoxal phosphate-dependent enzyme -
  LU301_RS04780 (LU301_04770) - 937557..938660 (+) 1104 WP_305273220.1 hypothetical protein -
  LU301_RS04785 (LU301_04775) - 938757..940778 (+) 2022 WP_305273968.1 FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38101.81 Da        Isoelectric Point: 6.8928

>NTDB_id=639625 LU301_RS04770 WP_305273216.1 935600..936637(-) (pilT) [Moraxella sp. ZY210820]
MDITELLAFSAKNGASDLHLSAGLPPMIRVDGEVRRINLPPMDHKDVHRLIYDIMNDKQRRDYEELLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMDQLGLGPIFKTIAEYPRGIVLVTGPTGSGKSTTLAAMLDYINDTRHDHILTVED
PIEFVHTSKKCLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGVPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KNLVTKGIISKEAARAAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=639625 LU301_RS04770 WP_305273216.1 935600..936637(-) (pilT) [Moraxella sp. ZY210820]
ATGGATATTACTGAATTATTGGCATTCTCAGCCAAAAATGGTGCATCAGACTTACACTTATCAGCAGGTTTACCGCCAAT
GATTCGTGTTGATGGTGAAGTACGACGTATTAACTTACCTCCAATGGATCATAAAGACGTTCACCGTTTAATCTATGATA
TCATGAACGATAAACAACGCCGTGATTATGAAGAATTATTAGAAACTGACTTTTCATTTGAAGTACCGGGAGTTGCACGT
TTCCGTGTAAACGCATTTAACCAAAACCGTGGTGCTGGTGCTGTATTTCGTACCATTCCCTCAAAAGTATTAACCATGGA
TCAATTAGGTTTAGGTCCTATTTTTAAAACTATTGCCGAGTATCCTCGTGGTATTGTATTAGTTACAGGTCCGACAGGTT
CTGGTAAATCAACAACTTTGGCTGCAATGTTAGACTATATTAATGACACACGTCATGACCATATTTTAACTGTTGAAGAT
CCAATTGAATTTGTACATACGTCTAAAAAATGTTTGATTAACCAACGTGAAGTACACCGTGATACTTTAGGTTTTAACGA
AGCATTACGTTCAGCACTGCGTGAAGATCCTGATATTATTCTAGTTGGTGAGATGCGTGACTTAGAAACCATTCGTTTAG
CATTAACTGCTGCAGAAACAGGACACTTGGTATTTGGTACGCTCCACACCACATCTGCAGCAAAAACCATTGACCGTGTA
ATTGACGTATTCCCTGCTGAAGAAAAAGATATGGTGCGTGCCATGTTATCAGAATCATTACAAGCGGTTATTTCACAAAC
ACTCTTAAAGAAAAATGGTGGCGGTCGTGTTGCTGCACATGAAATTATGATTGGTGTACCTGCGATTCGTAACTTAATTC
GTGAAAATAAAGTTGCACAGATGTATTCTGCAATCCAAACGGGTGCAAACCATGGTATGACTACACTTGACCAAAGTTTA
AAAAACCTTGTTACTAAAGGGATTATTAGTAAAGAAGCGGCCCGTGCTGCTGCCAAACAGCCAGAGTCTTTCTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

91.884

100

0.919
  pilT Acinetobacter baumannii D1279779

91.884

100

0.919
  pilT Acinetobacter baumannii strain A118

91.884

100

0.919
  pilT Acinetobacter baylyi ADP1

87.5

99.71

0.872
  pilT Pseudomonas aeruginosa PAK

84.884

99.71

0.846
  pilT Pseudomonas stutzeri DSM 10701

84.593

99.71

0.843
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Neisseria gonorrhoeae MS11

68.406

100

0.684
  pilT Neisseria meningitidis 8013

68.406

100

0.684
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.643

97.391

0.678
  pilT Vibrio cholerae strain A1552

69.643

97.391

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

39.884

100

0.4
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394