Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LVB87_RS02335 Genome accession   NZ_CP089925
Coordinates   489727..490839 (+) Length   370 a.a.
NCBI ID   WP_232899312.1    Uniprot ID   -
Organism   Lysobacter sp. KIS68-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 484727..495839
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVB87_RS02315 (LVB87_02315) - 484993..486162 (-) 1170 WP_232899308.1 cystathionine gamma-synthase -
  LVB87_RS02320 (LVB87_02320) - 486227..487597 (-) 1371 WP_232899309.1 pyridoxal-phosphate dependent enzyme -
  LVB87_RS02325 (LVB87_02325) - 487743..487961 (-) 219 WP_232899310.1 YdcH family protein -
  LVB87_RS02330 (LVB87_02330) - 488293..489618 (-) 1326 WP_232899311.1 OmpA family protein -
  LVB87_RS02335 (LVB87_02335) pilU 489727..490839 (+) 1113 WP_232899312.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LVB87_RS02340 (LVB87_02340) maiA 490844..491509 (-) 666 WP_232899313.1 maleylacetoacetate isomerase -
  LVB87_RS02345 (LVB87_02345) - 491602..492594 (-) 993 WP_232899314.1 fumarylacetoacetate hydrolase family protein -
  LVB87_RS02355 (LVB87_02355) rpsI 492802..493194 (-) 393 WP_232899315.1 30S ribosomal protein S9 -
  LVB87_RS02360 (LVB87_02360) rplM 493204..493632 (-) 429 WP_232899316.1 50S ribosomal protein L13 -
  LVB87_RS02365 (LVB87_02365) - 493778..494326 (-) 549 WP_232899317.1 hypothetical protein -
  LVB87_RS02370 (LVB87_02370) - 494328..494780 (-) 453 WP_232899318.1 EF-hand domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40803.19 Da        Isoelectric Point: 6.1879

>NTDB_id=639129 LVB87_RS02335 WP_232899312.1 489727..490839(+) (pilU) [Lysobacter sp. KIS68-7]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGKLYPLGNTGLPTGMVKKIAYSLMDEGQVPQFERDLELNMAIALPESGR
FRVNVFKQRGEVGMVIRAIRSVIPSIEELQLPQVLKEIIMAPRGLVLIVGSTGSGKSTTLASMIDYRNSTSPGHILTIED
PIEYLHKHKKSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHICLATLHSNNADQTIERI
LNFFPEAAHKNVLMNLALNLKAVVSQRLVIGVDGRRIPAAEVLINTPMIRDLLRRGQVHEIKQAMEESLEAGMESFDQCL
FRLAKEGKIDKEAALQAADSRDGLALKFRLSEGATGEHDPYADVFDTRVG

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=639129 LVB87_RS02335 WP_232899312.1 489727..490839(+) (pilU) [Lysobacter sp. KIS68-7]
ATGGACATCGGCTATTTCCTGAAGCTGATGACGGAGAAGAACGCTTCCGACATGTTCCTGACCACCGGCGCGCCGGTGTA
CATCAAGGTCGAAGGCAAGCTCTATCCGCTCGGGAACACCGGCCTGCCCACGGGCATGGTGAAGAAGATCGCGTACTCGC
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGACCTGGAGCTGAACATGGCGATCGCGCTCCCGGAGTCGGGCCGC
TTCCGCGTGAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTGATCCGCGCGATCCGCAGCGTGATCCCGTCCATCGA
AGAGCTGCAGCTGCCGCAGGTGCTGAAGGAAATCATCATGGCCCCGCGCGGCCTGGTGCTGATCGTCGGCTCCACCGGTT
CGGGCAAGTCGACCACGCTCGCTTCGATGATCGACTACCGCAACAGCACTTCGCCGGGCCACATCCTCACCATCGAGGAT
CCGATCGAATACCTGCACAAGCACAAGAAGTCGATCGTGAACCAGCGCGAAGTCGGCCTGGACACGCACGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGCGAAGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCGACGACGATGGAAGCGG
CGATCGCCTTCGCCGAAACCGGCCACATCTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAGCCGCGCACAAGAACGTGCTGATGAACCTCGCGCTGAACCTCAAGGCGGTGGTCTCGCAGCG
TTTGGTGATCGGCGTCGATGGCCGCCGCATCCCGGCGGCGGAAGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGATCAAGCAGGCGATGGAAGAGTCGCTGGAAGCGGGCATGGAGTCCTTCGACCAGTGCCTG
TTCCGCCTCGCGAAGGAAGGCAAGATCGACAAGGAAGCGGCGTTGCAGGCCGCGGACTCGCGCGACGGCCTCGCCCTCAA
GTTCCGCCTGTCCGAAGGCGCGACCGGCGAACACGATCCCTACGCGGACGTCTTCGACACCCGCGTCGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.532

97.027

0.5
  pilU Acinetobacter baylyi ADP1

49.02

96.486

0.473
  pilU Vibrio cholerae strain A1552

48.588

95.676

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.723

91.622

0.419
  pilT Pseudomonas aeruginosa PAK

42.308

91.351

0.386
  pilT Pseudomonas stutzeri DSM 10701

41.716

91.351

0.381
  pilT Legionella pneumophila strain ERS1305867

41.42

91.351

0.378
  pilT Acinetobacter nosocomialis M2

41.42

91.351

0.378
  pilT Acinetobacter baumannii D1279779

41.42

91.351

0.378
  pilT Acinetobacter baumannii strain A118

41.42

91.351

0.378
  pilT Legionella pneumophila strain Lp02

41.42

91.351

0.378
  pilT Acinetobacter baylyi ADP1

41.176

91.892

0.378
  pilT Neisseria gonorrhoeae MS11

39.769

93.784

0.373
  pilT Neisseria meningitidis 8013

39.481

93.784

0.37
  pilT Vibrio cholerae strain A1552

40.361

89.73

0.362
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.361

89.73

0.362