Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LUU92_RS27130 Genome accession   NZ_CP089519
Coordinates   5970430..5971464 (-) Length   344 a.a.
NCBI ID   WP_257102891.1    Uniprot ID   -
Organism   Pseudomonas extremorientalis strain 1502IPR-01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5965430..5976464
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LUU92_RS27100 (LUU92_27050) ruvX 5965529..5965966 (+) 438 WP_003195067.1 Holliday junction resolvase RuvX -
  LUU92_RS27105 (LUU92_27055) pyrR 5966040..5966546 (+) 507 WP_058426562.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  LUU92_RS27110 (LUU92_27060) - 5966571..5967575 (+) 1005 WP_058426561.1 aspartate carbamoyltransferase catalytic subunit -
  LUU92_RS27115 (LUU92_27065) - 5967572..5968843 (+) 1272 WP_257102890.1 dihydroorotase -
  LUU92_RS27120 (LUU92_27070) - 5968989..5969399 (-) 411 WP_257103878.1 TM2 domain-containing protein -
  LUU92_RS27125 (LUU92_27075) - 5969668..5970303 (+) 636 WP_071490370.1 C40 family peptidase -
  LUU92_RS27130 (LUU92_27080) pilT 5970430..5971464 (-) 1035 WP_257102891.1 type IV pilus twitching motility protein PilT Machinery gene
  LUU92_RS27135 (LUU92_27085) - 5971522..5972208 (+) 687 WP_092241976.1 YggS family pyridoxal phosphate-dependent enzyme -
  LUU92_RS27140 (LUU92_27090) proC 5972242..5973060 (+) 819 WP_257102892.1 pyrroline-5-carboxylate reductase -
  LUU92_RS27145 (LUU92_27095) - 5973070..5973660 (+) 591 WP_025859579.1 YggT family protein -
  LUU92_RS27150 (LUU92_27100) - 5973780..5974919 (+) 1140 WP_071490374.1 homoserine O-acetyltransferase -
  LUU92_RS27155 (LUU92_27105) metW 5974927..5975547 (+) 621 WP_034109956.1 methionine biosynthesis protein MetW -
  LUU92_RS27160 (LUU92_27110) - 5975569..5976006 (+) 438 WP_257102893.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37457.23 Da        Isoelectric Point: 6.8433

>NTDB_id=637928 LUU92_RS27130 WP_257102891.1 5970430..5971464(-) (pilT) [Pseudomonas extremorientalis strain 1502IPR-01]
MDITELLTASVRRGASDLHLSAGLAPMLRVDGEVWPLDGRVLSPPQVADLLSPLLNQHQQKDFETSLETDFAFELPGVAR
FRANVFRQERGMGAVFRAVPSEIQSLERLGLGEVFQRIAQLPRGLVLVTGPTGSGKSTTLAAMIDFLNQHRRQHILTLED
PIEFIHTPNMALINQRQVHRDTHSFSVALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRL
VDVFPAGEKAMVRSMLSESLQAVVSQVLVKKIGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGALGMKTLDMSL
KALVGEGVISREEAQQKARVPGEI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=637928 LUU92_RS27130 WP_257102891.1 5970430..5971464(-) (pilT) [Pseudomonas extremorientalis strain 1502IPR-01]
ATGGATATCACTGAATTACTCACGGCCAGCGTGCGCCGTGGCGCCTCCGACCTGCATTTGTCCGCCGGACTGGCGCCGAT
GCTGCGGGTGGATGGGGAGGTCTGGCCGCTTGATGGGCGGGTGCTTTCACCCCCGCAAGTGGCCGACTTGTTGAGCCCAT
TGCTCAATCAGCACCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCCTTCGAACTGCCCGGTGTGGCGCGG
TTCCGCGCCAATGTGTTCCGGCAGGAGCGGGGCATGGGCGCGGTGTTTCGCGCTGTACCGTCCGAGATCCAGAGCCTGGA
GCGCCTCGGCCTGGGGGAAGTATTCCAGCGTATCGCCCAGCTGCCGCGCGGCCTGGTGCTGGTGACCGGCCCTACCGGCT
CGGGCAAGTCCACTACCCTGGCGGCGATGATCGACTTTCTCAATCAGCATCGACGCCAGCACATCCTCACCCTTGAAGAC
CCCATCGAGTTTATCCACACGCCGAACATGGCATTGATCAACCAACGCCAGGTGCACCGCGACACCCATAGTTTTTCGGT
TGCCCTGCGCTCGGCGCTCAGGGAAGACCCGGACGTGATCCTGGTGGGTGAACTGCGCGACCTGGAAACCATCCGCCTGG
CCCTGACGGCGGCTGAGACCGGGCACCTGGTATTCGGCACCTTGCATACCTCGTCGGCGGCAAAGACCGTGGATCGGCTG
GTGGATGTGTTTCCGGCGGGGGAAAAGGCCATGGTCCGCTCGATGCTGTCGGAGTCGTTGCAGGCAGTGGTGTCCCAGGT
GCTGGTGAAGAAGATCGGCGGGGGGCGGGTGGCGGCCCATGAAATCATGTTGGGTACGCCGGCTATTCGCAATCTGATCC
GTGAGGACAAGGTGGCGCAGATGGTCTCGGCGATCCAGACGGGTGGGGCGCTGGGGATGAAGACGCTGGATATGAGCTTG
AAGGCGTTGGTCGGGGAGGGGGTGATCAGTCGGGAAGAGGCGCAGCAGAAGGCGAGGGTGCCTGGGGAAATATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

72.434

99.128

0.718
  pilT Pseudomonas stutzeri DSM 10701

70.381

99.128

0.698
  pilT Acinetobacter baumannii D1279779

68.622

99.128

0.68
  pilT Acinetobacter baumannii strain A118

68.622

99.128

0.68
  pilT Acinetobacter nosocomialis M2

68.622

99.128

0.68
  pilT Acinetobacter baylyi ADP1

67.742

99.128

0.672
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.985

97.965

0.637
  pilT Vibrio cholerae strain A1552

64.985

97.965

0.637
  pilT Legionella pneumophila strain Lp02

63.314

98.256

0.622
  pilT Legionella pneumophila strain ERS1305867

63.314

98.256

0.622
  pilT Neisseria meningitidis 8013

60.588

98.837

0.599
  pilT Neisseria gonorrhoeae MS11

60.294

98.837

0.596
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.406

100

0.485
  pilU Vibrio cholerae strain A1552

39.823

98.547

0.392
  pilU Pseudomonas stutzeri DSM 10701

40

97.384

0.39
  pilU Acinetobacter baylyi ADP1

38.439

100

0.387