Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LTQ54_RS10975 Genome accession   NZ_CP089203
Coordinates   2369918..2370955 (+) Length   345 a.a.
NCBI ID   WP_004735478.1    Uniprot ID   A0A1R3EAA6
Organism   Vibrio splendidus strain 2_C04b     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2364918..2375955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LTQ54_RS10940 (LTQ54_10940) hemW 2365036..2366223 (-) 1188 WP_017095270.1 radical SAM family heme chaperone HemW -
  LTQ54_RS10945 (LTQ54_10945) - 2366223..2366822 (-) 600 WP_243578766.1 XTP/dITP diphosphatase -
  LTQ54_RS10950 (LTQ54_10950) - 2366871..2367302 (-) 432 WP_004735472.1 DUF4426 domain-containing protein -
  LTQ54_RS10955 (LTQ54_10955) yggU 2367331..2367621 (-) 291 WP_243578767.1 DUF167 family protein YggU -
  LTQ54_RS10960 (LTQ54_10960) - 2367651..2368208 (-) 558 WP_004735475.1 YggT family protein -
  LTQ54_RS10965 (LTQ54_10965) proC 2368284..2369102 (-) 819 WP_108167423.1 pyrroline-5-carboxylate reductase -
  LTQ54_RS10970 (LTQ54_10970) - 2369187..2369891 (-) 705 WP_243578768.1 YggS family pyridoxal phosphate-dependent enzyme -
  LTQ54_RS10975 (LTQ54_10975) pilT 2369918..2370955 (+) 1038 WP_004735478.1 type IV pilus twitching motility protein PilT Machinery gene
  LTQ54_RS10980 (LTQ54_10980) pilU 2370970..2372079 (+) 1110 WP_004735479.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LTQ54_RS10985 (LTQ54_10985) - 2372138..2372299 (-) 162 WP_243578769.1 hypothetical protein -
  LTQ54_RS10990 (LTQ54_10990) ruvX 2372398..2372820 (-) 423 WP_004735480.1 Holliday junction resolvase RuvX -
  LTQ54_RS10995 (LTQ54_10995) - 2372882..2373445 (-) 564 WP_017095267.1 YqgE/AlgH family protein -
  LTQ54_RS11000 (LTQ54_11000) gshB 2373562..2374512 (-) 951 WP_004735482.1 glutathione synthase -
  LTQ54_RS11005 (LTQ54_11005) rsmE 2374522..2375253 (-) 732 WP_017077778.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38317.83 Da        Isoelectric Point: 6.0512

>NTDB_id=635707 LTQ54_RS10975 WP_004735478.1 2369918..2370955(+) (pilT) [Vibrio splendidus strain 2_C04b]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMSDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQSRGCSAVFRTIPVEIPTLEQLGAPEIFERISNYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVEKKIEIETSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=635707 LTQ54_RS10975 WP_004735478.1 2369918..2370955(+) (pilT) [Vibrio splendidus strain 2_C04b]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATACCAGCTTTGAGTCATGCTGATGTGCATCGTTTGGTTTTTGAGA
TCATGAGTGATTCACAACGCGGTGAATTTGAAGAAAAACTGGAAGTCGACTTCTCTTTTGAATTACCCAACGTTGGTCGT
TTCCGTGTTAATGCTTTTAACCAATCTCGTGGCTGCTCTGCTGTGTTCCGTACCATTCCAGTAGAGATTCCCACCCTTGA
ACAGCTAGGGGCTCCTGAGATCTTTGAAAGAATATCTAATTACGAAAAAGGGCTAGTATTGGTCACAGGGCCGACTGGTT
CAGGTAAGTCGACAACTTTGGCAGCGATGGTGGATTACGTTAACCGTAACCATAATAAGCATATATTGACGATTGAAGAC
CCGATCGAATTTGTTCATACCAACAACAAATGCTTAATCAACCAACGAGAAGTGCATCGCGATACTCACAGCTTCAAAGC
GGCATTGCGTAGTGCGTTGCGTGAAGATCCAGACGTAATCCTTGTCGGCGAACTTCGTGACCAAGAGACGATCAGCTTAG
CGCTAACCGCAGCAGAAACGGGTCACTTAGTTTTTGGTACCTTGCATACTAGCTCTGCGGCAAAAACCATAGACCGTATT
ATCGATGTATTCCCGGGTAGCGACAAAGACATGGTGCGTTCAATGTTGTCTGAATCGTTACGTTCGGTGATTGCCCAGAA
GCTGTTAAAGCGTGTTGGTGGCGGTCGTGTGGCTTGTCATGAAATCATGATGGCGACACCTGCGATTAGAAACTTGATCC
GTGAAGACAAGGTCGCGCAGATGTATTCGATCATTCAAACGGGCGCGGCACACGGTATGCAGACCATGGAGCAAAATGCG
AAGCAGCTGATGGCACAAGGCTTAGTTGATTCAGAAGAGGTCGAGAAAAAGATCGAAATTGAAACCTCAATGTTTTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.696

100

0.887
  pilT Vibrio cholerae strain A1552

88.696

100

0.887
  pilT Acinetobacter baumannii D1279779

72.59

96.232

0.699
  pilT Acinetobacter baumannii strain A118

72.59

96.232

0.699
  pilT Acinetobacter nosocomialis M2

72.289

96.232

0.696
  pilT Pseudomonas aeruginosa PAK

69.412

98.551

0.684
  pilT Acinetobacter baylyi ADP1

71.515

95.652

0.684
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Legionella pneumophila strain Lp02

65.507

100

0.655
  pilT Legionella pneumophila strain ERS1305867

65.507

100

0.655
  pilT Neisseria meningitidis 8013

62.609

100

0.626
  pilT Neisseria gonorrhoeae MS11

62.319

100

0.623
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.841

0.496
  pilU Vibrio cholerae strain A1552

42.769

94.203

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.812

0.388
  pilU Acinetobacter baylyi ADP1

39.688

92.754

0.368