Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LTQ03_RS09185 Genome accession   NZ_CP089201
Coordinates   1994617..1995726 (+) Length   369 a.a.
NCBI ID   WP_243583207.1    Uniprot ID   -
Organism   Vibrio splendidus strain 1_C04a     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1996055..2009022 1994617..1995726 flank 329


Gene organization within MGE regions


Location: 1994617..2009022
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LTQ03_RS09185 (LTQ03_09185) pilU 1994617..1995726 (+) 1110 WP_243583207.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LTQ03_RS09190 (LTQ03_09190) - 1995785..1995955 (-) 171 WP_243583208.1 hypothetical protein -
  LTQ03_RS09195 (LTQ03_09195) ruvX 1996055..1996477 (-) 423 WP_004735480.1 Holliday junction resolvase RuvX -
  LTQ03_RS09200 (LTQ03_09200) - 1996563..1997126 (-) 564 WP_004735481.1 YqgE/AlgH family protein -
  LTQ03_RS09205 (LTQ03_09205) gshB 1997243..1998193 (-) 951 WP_004735482.1 glutathione synthase -
  LTQ03_RS09210 (LTQ03_09210) rsmE 1998203..1998934 (-) 732 WP_243583209.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LTQ03_RS09215 (LTQ03_09215) - 1999094..1999825 (-) 732 WP_243583210.1 endonuclease -
  LTQ03_RS09220 (LTQ03_09220) - 1999923..2000423 (-) 501 WP_012604825.1 SprT family zinc-dependent metalloprotease -
  LTQ03_RS09225 (LTQ03_09225) - 2000502..2001299 (-) 798 WP_243583211.1 DUF2189 domain-containing protein -
  LTQ03_RS09230 (LTQ03_09230) metK 2001668..2002822 (-) 1155 WP_004735487.1 methionine adenosyltransferase -
  LTQ03_RS09235 (LTQ03_09235) tkt 2003200..2005194 (+) 1995 WP_243583212.1 transketolase -
  LTQ03_RS09240 (LTQ03_09240) epd 2005411..2006460 (+) 1050 WP_032545991.1 erythrose-4-phosphate dehydrogenase -
  LTQ03_RS09245 (LTQ03_09245) - 2006627..2007790 (+) 1164 WP_004735491.1 phosphoglycerate kinase -
  LTQ03_RS09250 (LTQ03_09250) fbaA 2007946..2009022 (+) 1077 WP_004735492.1 class II fructose-bisphosphate aldolase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41397.27 Da        Isoelectric Point: 5.8700

>NTDB_id=635661 LTQ03_RS09185 WP_243583207.1 1994617..1995726(+) (pilU) [Vibrio splendidus strain 1_C04a]
MELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGEKLNSAQVAQLLDAMMDQDRRDEYQQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGSGRHGVFEILLNSPRVSDLIRRGELHELKATMTKSKEIGMQTFDQALY
DLVIAGKISEEDAFHSADSANDLRLMLKTRRGDDDYGTGALAGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=635661 LTQ03_RS09185 WP_243583207.1 1994617..1995726(+) (pilU) [Vibrio splendidus strain 1_C04a]
ATGGAATTGAATCAAATCCTTGAGGGGATGCTATCTCAAAAAGCGTCGGATCTTTATATCACCGTTGATGCGCCAATTCT
GTTTCGTGTGGATGGTGAACTGCGACCTCAAGGCGAGAAATTGAATTCGGCTCAGGTTGCTCAGTTACTTGATGCGATGA
TGGATCAAGACCGACGCGATGAATATCAGCAAACGCGTGAGGCTAATTTTGCCATCGTGCGTGATTTTGGTCGTTTTCGT
GTGAGTGCGTTTTTTCAGCGAGAGCTACCAGGAGCGGTCATTCGTCGTATCGAAACTAACATCCCAACCTTTGAGCAATT
AAAGCTTCCTGATGTACTGCAAGACCTGTCAATCGCAAAGCGTGGATTGGTGCTGGTGGTTGGTGCTACGGGCTCTGGTA
AATCAACCTCAATGGCTGCGATGACGGGCTATCGTAATACCAATCGTTCAGGGCATATTTTGACGGTTGAAGATCCAATT
GAATTCGTTCACGAACATAAAAAATGTATCGTAACTCAACGTGAGGTTGGGCTAGACACCGAGAGCTATGAAGTTGCGCT
TAAGAACTCTTTACGCCAAGCACCGGATATGATCTTGATTGGCGAAATCCGTAGCCGTGAAACCATGGAATACGCGATGA
CCTTTGCTGAAACGGGCCACTTGTGTATGGCAACACTGCACGCGAATAATGCCAACCAAGCGTTAGAACGTATTCTTCAC
TTGGTGCCGAAAGAACAGAAAGAGCAGTTCTTGTTTGATCTGTCGATGAACCTGCGTGGTGTGGTCGCTCAGCAATTAAT
CCGAGATAAGAATGGCAGCGGTCGCCATGGTGTATTCGAGATTCTACTCAACAGCCCGCGAGTGTCTGACTTGATTCGTC
GTGGTGAGTTGCATGAACTAAAAGCGACTATGACTAAATCGAAAGAGATTGGTATGCAGACCTTTGACCAAGCTTTGTAT
GATTTAGTCATAGCAGGCAAGATCAGTGAAGAGGATGCGTTCCACAGTGCGGATTCTGCTAATGACTTACGCTTAATGCT
CAAAACCAGACGTGGTGATGACGACTACGGAACCGGGGCGTTGGCTGGTGTTAAAATTGATATGGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.239

99.729

0.84
  pilU Pseudomonas stutzeri DSM 10701

56.334

100

0.566
  pilU Acinetobacter baylyi ADP1

54.131

95.122

0.515
  pilT Acinetobacter baumannii strain A118

42.353

92.141

0.39
  pilT Acinetobacter baumannii D1279779

42.353

92.141

0.39
  pilT Acinetobacter nosocomialis M2

42.353

92.141

0.39
  pilT Legionella pneumophila strain ERS1305867

42.183

91.87

0.388
  pilT Legionella pneumophila strain Lp02

42.183

91.87

0.388
  pilT Acinetobacter baylyi ADP1

42.169

89.973

0.379
  pilT Pseudomonas aeruginosa PAK

41.791

90.786

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.901

87.805

0.377
  pilT Pseudomonas stutzeri DSM 10701

40.299

90.786

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Neisseria gonorrhoeae MS11

38.551

93.496

0.36
  pilT Neisseria meningitidis 8013

38.551

93.496

0.36