Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LRK53_RS04295 Genome accession   NZ_CP088923
Coordinates   998674..999780 (-) Length   368 a.a.
NCBI ID   WP_027492951.1    Uniprot ID   -
Organism   Rhodanobacter thiooxydans strain FW510-R12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 993674..1004780
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LRK53_RS04270 (LRK53_04270) - 994647..994955 (+) 309 WP_027492946.1 c-type cytochrome -
  LRK53_RS04275 (LRK53_04275) djlA 994952..995758 (+) 807 WP_027492947.1 co-chaperone DjlA -
  LRK53_RS04280 (LRK53_04280) - 995892..996530 (-) 639 WP_027492948.1 HAD hydrolase-like protein -
  LRK53_RS04285 (LRK53_04285) - 996530..997714 (-) 1185 WP_027492949.1 phosphoglycerate kinase -
  LRK53_RS04290 (LRK53_04290) maiA 997886..998548 (+) 663 WP_027492950.1 maleylacetoacetate isomerase -
  LRK53_RS04295 (LRK53_04295) pilU 998674..999780 (-) 1107 WP_027492951.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LRK53_RS04300 (LRK53_04300) - 999866..1000432 (+) 567 WP_037089804.1 DUF4398 domain-containing protein -
  LRK53_RS04305 (LRK53_04305) - 1000429..1001070 (+) 642 WP_027492952.1 hypothetical protein -
  LRK53_RS04310 (LRK53_04310) - 1001434..1001661 (+) 228 WP_027492953.1 YdcH family protein -
  LRK53_RS04315 (LRK53_04315) - 1001808..1003178 (+) 1371 WP_235642525.1 pyridoxal-phosphate dependent enzyme -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40954.23 Da        Isoelectric Point: 6.3528

>NTDB_id=634007 LRK53_RS04295 WP_027492951.1 998674..999780(-) (pilU) [Rhodanobacter thiooxydans strain FW510-R12]
MDIGYFLKLMVDKGASDMFLSTGAPVNIKVEGKLYPLGNTGLPGGMVKKIAYSLMDEGQVPQFERDLELNMALAVKEAGR
FRINVFKQRGEVGMVIRAIKSEIPSIEQLQLPSIFRDLIMEPRGLILVVGATGSGKSTTLAAMLDQRNSHSPGHILTIED
PIEYLHRHKKSIVNQREVGLDTHSYHEALKNAMREAPDVIMIGEIRDTDTMEAAIAFSETGHLCLATLHSNNADQTLERI
LNFFPESAHKNVLMNLALNLRAVISQRLVIGKDGRRIPATEVLLNTPLIRDMIRRGQIHEIKEAMDRSLQEGMQTFDQSL
YKLYKEGRVELEEALNKSDSRDGLALKIRLAEGGNSDEAMADPYGMGF

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=634007 LRK53_RS04295 WP_027492951.1 998674..999780(-) (pilU) [Rhodanobacter thiooxydans strain FW510-R12]
GTGGATATCGGCTACTTCCTCAAGCTTATGGTAGACAAGGGCGCCTCGGACATGTTCCTGTCCACCGGCGCCCCGGTGAA
TATCAAGGTCGAGGGCAAGCTGTATCCGCTGGGCAACACCGGCCTGCCCGGCGGCATGGTCAAGAAGATCGCCTATTCGC
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGCGACCTGGAGCTGAACATGGCGCTGGCGGTGAAGGAAGCCGGCCGT
TTCCGCATCAACGTATTCAAGCAGCGCGGCGAAGTGGGCATGGTGATCCGCGCGATCAAGAGCGAGATCCCCAGCATCGA
ACAGCTGCAGCTGCCCAGCATCTTCCGCGACCTGATCATGGAGCCGCGCGGGCTGATCCTGGTGGTCGGCGCCACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCAATGCTCGACCAGCGCAACTCCCATTCGCCCGGCCACATCCTCACCATCGAGGAC
CCGATCGAATACCTGCACCGGCACAAGAAGTCGATCGTCAACCAGCGCGAGGTGGGCCTGGACACCCACAGCTACCACGA
GGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCATGATCGGCGAGATCCGCGACACCGACACCATGGAGGCGG
CGATCGCCTTCTCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACTCCAACAACGCCGACCAGACGCTGGAGCGCATC
CTCAACTTCTTCCCCGAATCGGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCGGTGATCAGCCAGCG
CCTGGTGATCGGCAAGGACGGCCGCCGCATCCCGGCCACCGAGGTACTGCTGAACACCCCGTTGATCCGCGACATGATCC
GCCGCGGCCAGATCCACGAGATCAAGGAAGCGATGGACCGCAGCCTGCAGGAAGGCATGCAGACCTTCGACCAGTCGTTG
TACAAGCTGTACAAGGAAGGCCGCGTGGAACTGGAAGAGGCGCTCAACAAGTCCGATTCGCGCGACGGCCTGGCGCTTAA
GATCCGCCTCGCCGAAGGCGGCAACAGCGACGAGGCGATGGCCGATCCTTACGGGATGGGGTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.852

95.652

0.486
  pilU Vibrio cholerae strain A1552

48.37

100

0.484
  pilU Acinetobacter baylyi ADP1

49.58

97.011

0.481
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.953

92.12

0.405
  pilT Legionella pneumophila strain ERS1305867

41.813

92.935

0.389
  pilT Legionella pneumophila strain Lp02

41.813

92.935

0.389
  pilT Acinetobacter baylyi ADP1

42.814

90.761

0.389
  pilT Pseudomonas aeruginosa PAK

41.42

91.848

0.38
  pilT Acinetobacter baumannii D1279779

41.916

90.761

0.38
  pilT Acinetobacter nosocomialis M2

41.916

90.761

0.38
  pilT Acinetobacter baumannii strain A118

41.916

90.761

0.38
  pilT Pseudomonas stutzeri DSM 10701

41.124

91.848

0.378
  pilT Vibrio cholerae strain A1552

40.95

91.576

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.95

91.576

0.375
  pilT Neisseria gonorrhoeae MS11

38.905

94.293

0.367
  pilT Neisseria meningitidis 8013

38.905

94.293

0.367