Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PVLB_RS01645 Genome accession   NC_022738
Coordinates   363558..364556 (+) Length   332 a.a.
NCBI ID   WP_023378213.1    Uniprot ID   A0AAN0QYH6
Organism   Pseudomonas sp. VLB120     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 358558..369556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVLB_RS01615 (PVLB_01575) - 359035..359469 (-) 435 WP_023378207.1 DUF4426 domain-containing protein -
  PVLB_RS01620 (PVLB_01580) metW 359491..360111 (-) 621 WP_023378208.1 methionine biosynthesis protein MetW -
  PVLB_RS01625 (PVLB_01585) metX 360119..361258 (-) 1140 WP_023378209.1 homoserine O-succinyltransferase MetX -
  PVLB_RS01630 (PVLB_01590) - 361348..361938 (-) 591 WP_023378210.1 YggT family protein -
  PVLB_RS01635 (PVLB_01595) proC 361949..362767 (-) 819 WP_023378211.1 pyrroline-5-carboxylate reductase -
  PVLB_RS01640 (PVLB_01600) - 362806..363492 (-) 687 WP_023378212.1 YggS family pyridoxal phosphate-dependent enzyme -
  PVLB_RS01645 (PVLB_01605) pilT 363558..364556 (+) 999 WP_023378213.1 type IV pilus twitching motility protein PilT Machinery gene
  PVLB_RS01650 (PVLB_01610) - 364632..365258 (-) 627 WP_023378214.1 C40 family peptidase -
  PVLB_RS01655 (PVLB_01615) - 365427..365846 (+) 420 WP_023378215.1 NINE protein -
  PVLB_RS01660 (PVLB_01620) - 365888..366586 (-) 699 WP_023378216.1 SPOR domain-containing protein -
  PVLB_RS01665 (PVLB_01625) argS 366588..368324 (-) 1737 WP_023378217.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 35725.36 Da        Isoelectric Point: 6.6988

>NTDB_id=63364 PVLB_RS01645 WP_023378213.1 363558..364556(+) (pilT) [Pseudomonas sp. VLB120]
MDVTDLLVRAMAAGASDLHLAAGDVPLIRLDGELQRLDLPPLSATALHDGLAPLLAEAQCRQWAQGDELDLALDLPSLGR
IRLNLFRQSNGPAVAMRIIAQRIATLEALGLTEVFRAFAQHTDGLVLVGGPTGSGKSSTLAALVDQLNRERSLHIITLED
PIEVIHSCQRSLVNQREIGRHCRDFAQGLRSALRQDPDVIVIGELRDLETIRLALRAAETGHLVLATVHTRSAINSIDRL
VEVFAAEEKPLVRAMLAESLRLVVAQVLVKRRGGGRVAAREVLVATPAVRNLIREGRAAQLQSVMQSGAAAGMKTMEGAM
EALRAEGLIDAL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=63364 PVLB_RS01645 WP_023378213.1 363558..364556(+) (pilT) [Pseudomonas sp. VLB120]
ATGGACGTGACTGACCTCTTGGTCCGGGCGATGGCTGCAGGGGCGAGTGACCTGCACCTGGCCGCGGGCGACGTACCGCT
GATTCGCCTGGATGGCGAGCTGCAACGCCTGGATCTGCCGCCGTTGTCGGCGACGGCGCTGCACGACGGGCTGGCGCCTT
TGCTGGCCGAAGCGCAGTGTCGCCAGTGGGCGCAAGGTGATGAGCTGGACCTGGCGCTCGATCTCCCCTCGCTTGGGCGT
ATCCGCTTGAATCTGTTTCGCCAGTCGAATGGGCCTGCGGTGGCCATGCGGATCATTGCGCAACGAATCGCCACACTCGA
AGCGTTAGGCCTGACAGAAGTGTTTCGAGCTTTTGCCCAACATACCGATGGCCTCGTCCTGGTAGGTGGGCCGACCGGTA
GCGGGAAGTCCAGCACGCTGGCCGCGCTGGTCGACCAGCTCAATCGCGAGCGCTCGCTGCACATCATCACATTGGAAGAC
CCTATCGAAGTTATCCACAGTTGCCAGCGCAGCCTGGTCAACCAGCGCGAGATCGGACGCCACTGTCGTGACTTCGCCCA
GGGGTTGCGCAGTGCACTGCGCCAAGACCCGGATGTCATCGTGATCGGCGAACTGAGGGATCTCGAAACGATTCGTCTGG
CGCTCCGGGCTGCCGAGACGGGGCATCTGGTGCTGGCGACCGTCCATACCCGGTCGGCGATCAACAGCATCGACCGTCTG
GTGGAGGTGTTTGCCGCCGAGGAGAAGCCGTTGGTCAGGGCCATGCTGGCCGAATCGCTGCGCTTGGTGGTAGCGCAGGT
GCTGGTGAAACGAAGAGGTGGCGGCCGGGTGGCGGCGCGGGAAGTGTTGGTGGCGACGCCCGCTGTGCGTAACCTGATCC
GAGAGGGGCGAGCGGCGCAGTTGCAGTCCGTGATGCAGTCGGGGGCGGCGGCGGGGATGAAGACGATGGAAGGAGCGATG
GAGGCGTTGAGGGCAGAAGGGCTGATCGACGCGTTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.755

99.699

0.506
  pilT Vibrio cholerae strain A1552

50.755

99.699

0.506
  pilT Pseudomonas aeruginosa PAK

50.76

99.096

0.503
  pilT Acinetobacter baylyi ADP1

50.303

99.398

0.5
  pilT Pseudomonas stutzeri DSM 10701

50.152

99.096

0.497
  pilT Acinetobacter baumannii strain A118

49.848

99.096

0.494
  pilT Acinetobacter baumannii D1279779

49.848

99.096

0.494
  pilT Acinetobacter nosocomialis M2

49.544

99.096

0.491
  pilT Neisseria meningitidis 8013

48.182

99.398

0.479
  pilT Legionella pneumophila strain Lp02

48.328

99.096

0.479
  pilT Legionella pneumophila strain ERS1305867

48.328

99.096

0.479
  pilT Neisseria gonorrhoeae MS11

47.879

99.398

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.061

99.398

0.458
  pilU Pseudomonas stutzeri DSM 10701

37.879

99.398

0.377
  pilU Vibrio cholerae strain A1552

37.346

97.59

0.364
  pilF Thermus thermophilus HB27

31.25

100

0.361

Multiple sequence alignment