Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KDJ21_RS12315 Genome accession   NZ_CP088268
Coordinates   2538009..2539049 (+) Length   346 a.a.
NCBI ID   WP_212136829.1    Uniprot ID   -
Organism   Metabacillus litoralis strain NCTR108     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2533009..2544049
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KDJ21_RS12300 (KDJ21_012300) - 2534189..2534980 (+) 792 WP_212136833.1 PRC-barrel domain-containing protein -
  KDJ21_RS12305 (KDJ21_012305) - 2534997..2536313 (+) 1317 WP_212136831.1 VanW family protein -
  KDJ21_RS12310 (KDJ21_012310) - 2536338..2537999 (+) 1662 WP_121662983.1 GspE/PulE family protein -
  KDJ21_RS12315 (KDJ21_012315) pilT 2538009..2539049 (+) 1041 WP_212136829.1 type IV pilus twitching motility protein PilT Machinery gene
  KDJ21_RS12320 (KDJ21_012320) - 2539049..2540256 (+) 1208 Protein_2337 type II secretion system F family protein -
  KDJ21_RS12325 (KDJ21_012325) - 2540350..2540805 (+) 456 WP_212136827.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  KDJ21_RS12330 (KDJ21_012330) - 2540889..2541665 (+) 777 WP_212136825.1 prepilin peptidase -
  KDJ21_RS12335 (KDJ21_012335) pilM 2541681..2542676 (+) 996 WP_212136823.1 type IV pilus biogenesis protein PilM -
  KDJ21_RS12340 (KDJ21_012340) - 2542683..2543267 (+) 585 WP_212136821.1 PilN domain-containing protein -
  KDJ21_RS12345 (KDJ21_012345) - 2543264..2544043 (+) 780 WP_212136819.1 hypothetical protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 37863.65 Da        Isoelectric Point: 7.9669

>NTDB_id=633631 KDJ21_RS12315 WP_212136829.1 2538009..2539049(+) (pilT) [Metabacillus litoralis strain NCTR108]
MKAKIDSILKLAYEKQASDIHLSVGVPPVMRINGELVRIGENMLMPADTEEMAKGMISSAKWSLFEEKGELDFSYGIPGV
SRFRVNAFRQRSCISLAVRIVPRNIPSIDDLQLPSILKSIVEKPHGLILVTGPTGSGKSTTLASMISFMNQTMKRHVITL
EDPIEYLHKHQKCIVDQREVGFDTGSFASGLKAALRQDPDVILVGELRDLETIHTAITAAETGHLVLGTLHTSSAASTVD
RIIDVFPANQQPQIRLQLASVLVGVISQRLFPTVDKSGRRAVTEILLNNSAVASLIRNEKTYQIPSVIQTSRAQGMQTFE
SSLSEYVRAGIISSEAALPYKQGTTD

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=633631 KDJ21_RS12315 WP_212136829.1 2538009..2539049(+) (pilT) [Metabacillus litoralis strain NCTR108]
TTGAAAGCAAAAATTGACTCTATATTAAAGCTTGCTTATGAAAAGCAAGCTTCAGACATTCATTTGTCAGTTGGTGTGCC
TCCCGTTATGCGAATTAACGGCGAGTTAGTTCGAATTGGCGAAAACATGTTAATGCCTGCTGATACAGAAGAAATGGCAA
AGGGAATGATTTCTTCAGCAAAATGGTCATTGTTTGAAGAAAAAGGAGAGCTTGATTTTTCTTACGGAATTCCAGGTGTA
TCTCGCTTTCGTGTTAATGCATTTCGCCAGCGTTCTTGTATCTCTCTAGCTGTACGAATTGTTCCAAGAAATATTCCATC
AATTGACGATTTGCAGTTGCCATCGATTTTAAAATCAATTGTGGAAAAGCCCCATGGACTTATCTTAGTAACTGGACCAA
CCGGCAGTGGAAAATCAACAACACTTGCGTCGATGATTTCCTTTATGAATCAAACGATGAAGCGCCATGTTATTACACTT
GAGGATCCGATTGAATATTTGCATAAACATCAAAAATGTATTGTTGATCAACGTGAAGTTGGTTTTGATACAGGTAGCTT
TGCATCAGGTTTAAAAGCAGCACTAAGACAGGATCCAGATGTGATTTTAGTTGGAGAGCTTCGCGATTTAGAAACAATTC
ACACAGCAATAACTGCAGCAGAAACAGGGCACCTTGTTCTTGGTACACTTCACACGTCATCAGCTGCATCAACAGTTGAT
CGGATTATCGATGTGTTTCCGGCTAATCAGCAGCCACAAATCCGACTTCAGCTTGCTTCCGTGTTAGTAGGGGTTATATC
ACAAAGGCTTTTCCCGACAGTTGATAAATCAGGGCGCCGTGCTGTAACTGAAATTTTATTAAATAACTCGGCAGTTGCCA
GCTTAATACGAAATGAAAAAACATACCAAATTCCGAGCGTGATTCAAACAAGTCGAGCACAAGGAATGCAAACCTTTGAA
AGCTCTCTTTCAGAGTATGTAAGAGCAGGCATTATTTCTTCTGAAGCAGCCTTACCATACAAACAAGGGACGACTGACTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(46-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.198

96.532

0.494
  pilT Neisseria gonorrhoeae MS11

48.503

96.532

0.468
  pilT Neisseria meningitidis 8013

48.204

96.532

0.465
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.64

95.665

0.465
  pilT Vibrio cholerae strain A1552

48.64

95.665

0.465
  pilT Legionella pneumophila strain Lp02

47.147

96.243

0.454
  pilT Acinetobacter baumannii D1279779

47.147

96.243

0.454
  pilT Acinetobacter baumannii strain A118

47.147

96.243

0.454
  pilT Acinetobacter nosocomialis M2

47.147

96.243

0.454
  pilT Legionella pneumophila strain ERS1305867

47.147

96.243

0.454
  pilT Pseudomonas stutzeri DSM 10701

46.547

96.243

0.448
  pilT Pseudomonas aeruginosa PAK

46.547

96.243

0.448
  pilT Acinetobacter baylyi ADP1

45.897

95.087

0.436
  pilU Pseudomonas stutzeri DSM 10701

42.566

99.133

0.422
  pilU Acinetobacter baylyi ADP1

41.543

97.399

0.405
  pilU Vibrio cholerae strain A1552

40.785

95.665

0.39