Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LQ771_RS12200 Genome accession   NZ_CP088252
Coordinates   2662470..2663585 (+) Length   371 a.a.
NCBI ID   WP_231349682.1    Uniprot ID   -
Organism   Frateuria soli strain 5GH9-11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2657470..2668585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LQ771_RS12180 (LQ771_12180) - 2658986..2660356 (-) 1371 WP_231349678.1 pyridoxal-phosphate dependent enzyme -
  LQ771_RS12185 (LQ771_12185) - 2660568..2660795 (-) 228 WP_231349679.1 YdcH family protein -
  LQ771_RS12190 (LQ771_12190) - 2661141..2661800 (-) 660 WP_231349680.1 hypothetical protein -
  LQ771_RS12195 (LQ771_12195) - 2661797..2662372 (-) 576 WP_231349681.1 DUF4398 domain-containing protein -
  LQ771_RS12200 (LQ771_12200) pilU 2662470..2663585 (+) 1116 WP_231349682.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LQ771_RS12205 (LQ771_12205) maiA 2663820..2664479 (-) 660 WP_231349683.1 maleylacetoacetate isomerase -
  LQ771_RS12210 (LQ771_12210) - 2664669..2665847 (+) 1179 WP_231349684.1 phosphoglycerate kinase -
  LQ771_RS12215 (LQ771_12215) - 2665847..2666491 (+) 645 WP_231349685.1 HAD hydrolase-like protein -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41103.43 Da        Isoelectric Point: 6.5096

>NTDB_id=633551 LQ771_RS12200 WP_231349682.1 2662470..2663585(+) (pilU) [Frateuria soli strain 5GH9-11]
MDIGYFLKLMVDKGASDMFLTTGAPVNIKVEGKLYPLGNTGLPGGMVKKIAYSLMDEGQVPQFERDLELNMALAVKEAGR
FRINVFKQRGEVGMVIRAIKSEIPSIDQLQLPTIFRDLIMEQRGLILVVGATGSGKSTTLAAMLDHRNSNSPGHILTIED
PIEYLHRHKKSIINQREVGLDTHSYHEALKNAMREAPDVIMIGEIRDTDTMEAAVAFSETGHLCLATLHSNNADQTLERI
LNFFPESAHKNVLMNLALNLRAVISQRLVIGKDGRRVPAVEVLLNTPLIRDMIRRGQIHEIKEAMDRSLQEGMQTFDQSL
YKLYKSGRIELEEALAKADSRDGLALKIRLSEGGGAEATELVGNDPYGLGF

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=633551 LQ771_RS12200 WP_231349682.1 2662470..2663585(+) (pilU) [Frateuria soli strain 5GH9-11]
GTGGATATCGGTTACTTCCTCAAGCTGATGGTAGACAAGGGCGCGTCGGACATGTTCCTGACCACCGGCGCCCCGGTGAA
CATCAAGGTCGAGGGCAAGCTCTACCCGCTGGGCAACACCGGCCTGCCTGGCGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGACGAGGGCCAGGTACCCCAGTTCGAGCGCGACCTGGAGCTCAACATGGCGCTGGCGGTGAAGGAAGCCGGTCGC
TTCCGCATCAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTGATCCGCGCGATCAAGAGCGAGATCCCCTCGATCGA
CCAACTGCAGCTGCCGACCATCTTCCGCGACCTGATCATGGAGCAGCGCGGCCTGATCCTGGTCGTCGGCGCCACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGCTCGACCATCGCAACTCCAACTCGCCGGGCCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACCGCCACAAGAAGTCGATCATCAACCAGCGCGAGGTCGGGCTGGACACGCACAGCTACCACGA
GGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCATGATCGGCGAGATCCGCGACACCGACACGATGGAGGCGG
CGGTCGCCTTCTCCGAAACCGGCCACCTGTGCCTGGCGACGCTGCATTCGAACAACGCCGACCAGACGCTGGAGCGCATC
CTCAACTTCTTCCCCGAATCGGCGCACAAGAACGTGCTGATGAACCTGGCGCTCAACCTGCGCGCGGTGATCAGCCAGCG
CCTGGTGATCGGCAAGGACGGTCGCCGCGTGCCGGCGGTGGAGGTGCTGCTCAACACGCCGCTGATCCGCGACATGATCC
GCCGTGGCCAGATCCACGAGATCAAGGAAGCGATGGACCGCAGCCTGCAGGAAGGCATGCAGACCTTCGACCAGTCGCTG
TACAAGCTCTACAAGAGCGGGCGCATCGAACTGGAGGAGGCGCTGGCCAAGGCCGACTCGCGCGATGGCCTGGCGCTGAA
GATCCGCCTGTCCGAAGGCGGCGGTGCCGAAGCCACCGAACTGGTCGGAAACGATCCGTACGGGTTGGGGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.24

97.844

0.501
  pilU Acinetobacter baylyi ADP1

51.695

95.418

0.493
  pilU Vibrio cholerae strain A1552

47.978

100

0.48
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.751

97.574

0.437
  pilT Acinetobacter baylyi ADP1

42.647

91.644

0.391
  pilT Acinetobacter baumannii strain A118

42.012

91.105

0.383
  pilT Acinetobacter baumannii D1279779

42.012

91.105

0.383
  pilT Acinetobacter nosocomialis M2

42.012

91.105

0.383
  pilT Pseudomonas aeruginosa PAK

41.124

91.105

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.828

91.105

0.372
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.356

90.836

0.367
  pilT Vibrio cholerae strain A1552

40.356

90.836

0.367
  pilT Legionella pneumophila strain Lp02

41.796

87.062

0.364
  pilT Legionella pneumophila strain ERS1305867

41.796

87.062

0.364
  pilT Neisseria meningitidis 8013

38.905

93.531

0.364
  pilT Neisseria gonorrhoeae MS11

38.905

93.531

0.364