Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LQ772_RS13615 Genome accession   NZ_CP088251
Coordinates   2934072..2935187 (+) Length   371 a.a.
NCBI ID   WP_231321500.1    Uniprot ID   -
Organism   Frateuria edaphi strain 5GH9-34     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2929072..2940187
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LQ772_RS13595 (LQ772_13595) - 2930631..2932001 (-) 1371 WP_231321496.1 pyridoxal-phosphate dependent enzyme -
  LQ772_RS13600 (LQ772_13600) - 2932174..2932401 (-) 228 WP_231321497.1 YdcH family protein -
  LQ772_RS13605 (LQ772_13605) - 2932746..2933402 (-) 657 WP_231321498.1 hypothetical protein -
  LQ772_RS13610 (LQ772_13610) - 2933399..2933974 (-) 576 WP_231321499.1 DUF4398 domain-containing protein -
  LQ772_RS13615 (LQ772_13615) pilU 2934072..2935187 (+) 1116 WP_231321500.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LQ772_RS13620 (LQ772_13620) mnhG 2935336..2935677 (-) 342 WP_231321501.1 monovalent cation/H(+) antiporter subunit G -
  LQ772_RS13625 (LQ772_13625) - 2935674..2935955 (-) 282 WP_231321502.1 K+/H+ antiporter subunit F -
  LQ772_RS13630 (LQ772_13630) - 2935952..2936437 (-) 486 WP_231321503.1 Na+/H+ antiporter subunit E -
  LQ772_RS13635 (LQ772_13635) - 2936434..2937987 (-) 1554 WP_231321505.1 monovalent cation/H+ antiporter subunit D -
  LQ772_RS13640 (LQ772_13640) - 2937984..2938322 (-) 339 WP_231321507.1 Na+/H+ antiporter subunit C -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41124.45 Da        Isoelectric Point: 6.4241

>NTDB_id=633528 LQ772_RS13615 WP_231321500.1 2934072..2935187(+) (pilU) [Frateuria edaphi strain 5GH9-34]
MDIGYFLKLMVDKGASDMFLTTGAPVNIKVEGKLYPLGNTGLPSGMVKKIAYSLMDEGQVPQFERDLELNMALAVKEAGR
FRINVFKQRGEVGMVIRAIKSEIPSIDQLQLPTIFRDLIMEQRGLILVVGATGSGKSTTLAAMLDQRNSNSPGHILTIED
PIEYLHRHKKSIINQREVGLDTHSYHEALKNAMREAPDVIMIGEIRDTDTMEAAVAFSETGHLCLATLHSNNADQTLERI
LNFFPESAHKNVLMNLALNLRAVISQRLVIGKDGRRVPAVEVLLNTPLIRDMIRRGQIHEIKEAMDRSLQEGMQTFDQSL
YKLYKSGRIELEEALAKADSRDGLALKIRLSEGGGAEATELVGNDPYGLGF

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=633528 LQ772_RS13615 WP_231321500.1 2934072..2935187(+) (pilU) [Frateuria edaphi strain 5GH9-34]
GTGGATATCGGTTACTTCCTCAAGCTGATGGTAGACAAGGGCGCGTCGGACATGTTCCTGACCACCGGCGCCCCGGTGAA
CATCAAGGTCGAGGGCAAGCTCTACCCGCTCGGCAACACCGGCCTGCCCAGCGGCATGGTCAAGAAGATCGCCTATTCGC
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGCGACCTGGAATTGAACATGGCGCTGGCGGTGAAGGAAGCCGGCCGC
TTCCGCATCAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTGATCCGCGCGATCAAGAGCGAGATTCCCTCGATCGA
CCAGCTGCAACTGCCGACCATCTTCCGCGATCTGATCATGGAGCAGCGCGGCCTGATCCTGGTGGTCGGCGCTACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGCTCGACCAGCGCAATTCCAATTCGCCCGGCCACATCCTCACCATCGAGGAC
CCGATCGAATACCTGCACCGGCACAAGAAGTCGATCATCAACCAGCGCGAGGTCGGGCTGGATACGCACAGCTATCACGA
GGCTCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCATGATCGGCGAGATCCGCGACACCGACACGATGGAGGCGG
CGGTCGCGTTCTCCGAAACCGGCCACCTGTGCCTGGCCACGTTGCACTCGAACAACGCCGACCAGACGCTGGAGCGCATC
CTCAACTTCTTCCCCGAATCGGCGCACAAGAACGTGCTGATGAACCTGGCGCTCAACCTGCGCGCGGTGATCAGCCAGCG
CCTGGTGATCGGCAAGGACGGGCGCCGCGTGCCGGCGGTGGAAGTGCTGCTCAACACGCCGCTGATCCGCGACATGATCC
GCCGCGGCCAGATCCACGAGATCAAGGAAGCGATGGACCGCAGCCTGCAGGAAGGCATGCAGACCTTCGACCAGTCGCTG
TACAAGCTCTACAAGAGCGGCCGCATCGAGCTGGAGGAAGCGCTGGCCAAGGCCGACTCGCGCGATGGCCTGGCGCTGAA
GATCCGCCTGTCCGAGGGCGGCGGGGCCGAGGCCACCGAACTGGTCGGGAACGATCCGTACGGCCTGGGGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(46-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.24

97.844

0.501
  pilU Acinetobacter baylyi ADP1

51.13

95.418

0.488
  pilU Vibrio cholerae strain A1552

47.978

100

0.48
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.475

97.574

0.434
  pilT Acinetobacter baylyi ADP1

42.647

91.644

0.391
  pilT Acinetobacter baumannii D1279779

42.012

91.105

0.383
  pilT Acinetobacter nosocomialis M2

42.012

91.105

0.383
  pilT Acinetobacter baumannii strain A118

42.012

91.105

0.383
  pilT Pseudomonas aeruginosa PAK

41.124

91.105

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.828

91.105

0.372
  pilT Vibrio cholerae strain A1552

40.356

90.836

0.367
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.356

90.836

0.367
  pilT Legionella pneumophila strain Lp02

41.796

87.062

0.364
  pilT Legionella pneumophila strain ERS1305867

41.796

87.062

0.364
  pilT Neisseria meningitidis 8013

38.905

93.531

0.364
  pilT Neisseria gonorrhoeae MS11

38.905

93.531

0.364