Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LPC09_RS18345 Genome accession   NZ_CP088245
Coordinates   3597931..3598971 (-) Length   346 a.a.
NCBI ID   WP_231307943.1    Uniprot ID   -
Organism   Metabacillus sp. B2-18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3592931..3603971
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPC09_RS18320 (LPC09_18320) - 3593697..3594281 (-) 585 WP_098795929.1 hypothetical protein -
  LPC09_RS18325 (LPC09_18325) pilM 3594288..3595283 (-) 996 WP_098795928.1 pilus assembly protein PilM -
  LPC09_RS18330 (LPC09_18330) - 3595299..3596072 (-) 774 WP_231307940.1 A24 family peptidase -
  LPC09_RS18335 (LPC09_18335) - 3596153..3596629 (-) 477 WP_231307941.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LPC09_RS18340 (LPC09_18340) - 3596723..3597931 (-) 1209 WP_231307942.1 type II secretion system F family protein -
  LPC09_RS18345 (LPC09_18345) pilT 3597931..3598971 (-) 1041 WP_231307943.1 type IV pilus twitching motility protein PilT Machinery gene
  LPC09_RS18350 (LPC09_18350) - 3598981..3600642 (-) 1662 WP_231307944.1 ATPase, T2SS/T4P/T4SS family -
  LPC09_RS18355 (LPC09_18355) - 3600667..3601992 (-) 1326 WP_098795922.1 G5 domain-containing protein -
  LPC09_RS18360 (LPC09_18360) - 3602010..3602801 (-) 792 WP_098795921.1 PRC-barrel domain-containing protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 37861.74 Da        Isoelectric Point: 8.4837

>NTDB_id=633483 LPC09_RS18345 WP_231307943.1 3597931..3598971(-) (pilT) [Metabacillus sp. B2-18]
MKAKIDSILKLAFEKQASDIHLSVGVPPVMRMNGELVRIGESMLMPADTEEMAKAMISSAKWSLFEEKGELDFSYGIPGV
SRFRVNAFRQRSCISLAVRIVPRNIPSIDDLQLPSILKSIVEKPHGLILVTGPTGSGKSTTLASMISFMNQTMKRHVITL
EDPIEYLHKHHKCIVDQREVGFDTGSFASGLKAALRQDPDVILVGELRDLETIHTAITAAETGHLVLGTLHTSSAASTVD
RIIDVFPANQQPQIRLQLASVLVGVISQRLFPTVDKSGRRAVTEILLNNSAVASIIRNEKTYQIPSVIQTSRAQGMRTFE
SSLSEYVKAGIISSEAALPYKQGTTD

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=633483 LPC09_RS18345 WP_231307943.1 3597931..3598971(-) (pilT) [Metabacillus sp. B2-18]
TTGAAGGCAAAAATTGACTCTATATTAAAGCTTGCTTTTGAAAAGCAAGCTTCAGACATTCATTTGTCAGTTGGTGTACC
TCCTGTTATGAGAATGAACGGCGAGTTAGTTCGAATTGGCGAAAGCATGTTAATGCCTGCTGATACAGAAGAAATGGCAA
AGGCAATGATTTCTTCAGCAAAATGGTCATTGTTTGAAGAAAAAGGAGAGCTTGATTTTTCTTACGGAATTCCAGGTGTA
TCTCGTTTTCGTGTTAATGCGTTTCGCCAGCGTTCTTGTATTTCCTTAGCTGTACGAATTGTCCCAAGGAATATTCCATC
AATAGACGATTTACAGTTGCCATCGATTTTAAAATCAATTGTGGAAAAGCCTCATGGACTGATCTTAGTAACTGGACCAA
CCGGCAGTGGAAAATCAACAACACTTGCGTCGATGATTTCCTTTATGAATCAAACGATGAAGCGCCATGTTATTACACTT
GAGGATCCGATTGAATATTTGCATAAACATCATAAATGCATTGTTGATCAACGTGAAGTTGGTTTTGATACAGGTAGCTT
TGCATCAGGTTTAAAAGCAGCACTAAGACAAGATCCAGATGTGATTTTAGTTGGAGAGCTTCGTGATTTAGAAACAATTC
ACACAGCAATAACGGCAGCAGAAACAGGACATCTTGTTCTTGGTACACTTCACACGTCGTCTGCTGCATCAACGGTTGAT
CGGATTATTGATGTGTTTCCGGCTAACCAGCAGCCACAAATTCGACTTCAGCTAGCTTCGGTGTTAGTTGGAGTTATTTC
ACAAAGACTTTTCCCGACTGTTGATAAATCAGGGCGCCGTGCTGTTACAGAAATCCTATTGAATAATTCGGCAGTTGCCA
GCATAATACGTAATGAAAAAACATACCAAATTCCGAGCGTGATTCAAACAAGCCGGGCACAAGGAATGAGAACCTTTGAA
AGCTCTCTTTCAGAATATGTTAAAGCAGGAATTATTTCTTCTGAAGCAGCTTTACCATACAAACAAGGGACGACAGATTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.599

96.532

0.488
  pilT Neisseria gonorrhoeae MS11

47.605

96.532

0.46
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.734

95.665

0.457
  pilT Vibrio cholerae strain A1552

47.734

95.665

0.457
  pilT Neisseria meningitidis 8013

47.305

96.532

0.457
  pilT Pseudomonas stutzeri DSM 10701

46.588

97.399

0.454
  pilT Acinetobacter baumannii D1279779

46.847

96.243

0.451
  pilT Acinetobacter baumannii strain A118

46.847

96.243

0.451
  pilT Acinetobacter nosocomialis M2

46.847

96.243

0.451
  pilT Legionella pneumophila strain ERS1305867

46.547

96.243

0.448
  pilT Legionella pneumophila strain Lp02

46.547

96.243

0.448
  pilT Pseudomonas aeruginosa PAK

45.946

96.243

0.442
  pilT Acinetobacter baylyi ADP1

45.593

95.087

0.434
  pilU Pseudomonas stutzeri DSM 10701

41.399

99.133

0.41
  pilU Acinetobacter baylyi ADP1

40.95

97.399

0.399
  pilU Vibrio cholerae strain A1552

40.483

95.665

0.387