Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LPW11_RS17645 Genome accession   NZ_CP087999
Coordinates   4129811..4130908 (+) Length   365 a.a.
NCBI ID   WP_230995190.1    Uniprot ID   -
Organism   Geomonas sp. RF6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4124811..4135908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPW11_RS17630 (LPW11_17630) - 4125303..4127168 (+) 1866 WP_230995188.1 DUF2723 domain-containing protein -
  LPW11_RS17635 (LPW11_17635) - 4127165..4128055 (+) 891 WP_230998315.1 shikimate dehydrogenase -
  LPW11_RS17640 (LPW11_17640) pilB 4128089..4129795 (+) 1707 WP_230995189.1 type IV-A pilus assembly ATPase PilB -
  LPW11_RS17645 (LPW11_17645) pilT 4129811..4130908 (+) 1098 WP_230995190.1 type IV pilus twitching motility protein PilT Machinery gene
  LPW11_RS17650 (LPW11_17650) - 4131145..4132362 (+) 1218 WP_230995191.1 type II secretion system F family protein -
  LPW11_RS17655 (LPW11_17655) - 4132366..4133973 (+) 1608 WP_230995192.1 ATP-binding protein -
  LPW11_RS17660 (LPW11_17660) - 4134029..4135402 (+) 1374 WP_230995193.1 sigma-54 dependent transcriptional regulator -
  LPW11_RS17665 (LPW11_17665) - 4135624..4135833 (+) 210 WP_230995194.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 40638.86 Da        Isoelectric Point: 7.2922

>NTDB_id=632226 LPW11_RS17645 WP_230995190.1 4129811..4130908(+) (pilT) [Geomonas sp. RF6]
MINFHQLLKDLVERGGSDLHITTNTPPQIRIDGKLTPMDIPPLNAIETKQLCYSILTDAQKHKFEEENELDLSFGVKGLS
RFRGNIFIQRGAVAGVFRVIPYRILSFEELGLPPVVTELCGKPRGLILVTGPTGSGKSTTLASMIDKINTDRHDHIVTIE
DPIEYLHPHKGCIVNQREVGADTKSFKHALKYVLRQDPDVVLVGELRDLETIEAALTLAETGHLCFATLHTNSCAQTINR
IIDVFPSYQQTQVRTQLSFVLEGVLSQMLVPRSDGRGRALALEVMVPNPAIRNLVREDKVHQIYSQMQVGQEKFGMQTMN
QSLVAHLAKRNISIDDAIARSPDPDELKQMLSPGGAAMGQRRPMR

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=632226 LPW11_RS17645 WP_230995190.1 4129811..4130908(+) (pilT) [Geomonas sp. RF6]
ATGATCAACTTTCACCAGCTACTGAAGGATCTCGTGGAGCGGGGCGGCTCCGACCTGCACATCACCACGAACACGCCGCC
GCAGATCCGCATCGACGGGAAGCTCACCCCGATGGACATCCCCCCCCTGAACGCCATCGAGACGAAGCAGCTGTGCTACA
GCATCCTCACCGACGCGCAGAAACACAAGTTCGAGGAGGAGAACGAGCTCGACCTCTCTTTCGGGGTAAAGGGGCTCTCC
CGCTTCCGCGGCAACATCTTCATCCAGCGCGGCGCGGTGGCAGGGGTCTTCAGGGTCATCCCGTACCGGATCCTCTCCTT
CGAGGAACTGGGGCTGCCGCCGGTGGTAACGGAGCTGTGCGGGAAGCCGCGCGGCCTTATCCTCGTCACCGGCCCCACGG
GGAGCGGGAAGTCCACCACACTCGCCTCCATGATCGACAAGATCAACACGGACCGGCACGACCACATCGTGACGATCGAG
GACCCGATCGAGTACCTGCACCCGCACAAGGGGTGCATCGTGAACCAGCGCGAGGTAGGGGCCGACACGAAGAGCTTCAA
GCATGCGCTTAAGTACGTGCTGCGCCAGGACCCGGACGTCGTCCTCGTCGGCGAGTTGCGCGACCTGGAGACGATCGAGG
CGGCGCTCACCCTCGCCGAGACGGGGCACCTCTGCTTCGCCACGCTGCACACCAATTCGTGCGCCCAGACCATAAACCGC
ATCATCGACGTCTTCCCCTCCTACCAGCAGACCCAGGTGCGCACCCAGCTCTCCTTCGTCCTGGAGGGGGTCCTCTCCCA
GATGCTCGTGCCGCGCTCCGACGGCCGAGGGCGTGCGCTGGCGCTGGAGGTCATGGTGCCGAACCCGGCGATCAGGAACC
TCGTCAGGGAGGACAAGGTGCACCAGATCTACTCCCAGATGCAGGTCGGCCAGGAGAAGTTCGGCATGCAGACGATGAAC
CAGTCTCTCGTGGCGCACCTCGCGAAGCGGAACATCTCCATCGATGACGCCATCGCCCGCTCCCCCGACCCGGACGAGCT
GAAGCAGATGCTCTCTCCCGGCGGGGCCGCCATGGGGCAGCGCCGGCCGATGAGGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.721

98.082

0.488
  pilT Legionella pneumophila strain Lp02

53.776

90.685

0.488
  pilT Legionella pneumophila strain ERS1305867

53.776

90.685

0.488
  pilT Acinetobacter baylyi ADP1

51.453

94.247

0.485
  pilT Pseudomonas aeruginosa PAK

49.711

94.795

0.471
  pilT Pseudomonas stutzeri DSM 10701

49.422

94.795

0.468
  pilT Acinetobacter baumannii D1279779

49.133

94.795

0.466
  pilT Acinetobacter baumannii strain A118

49.133

94.795

0.466
  pilT Acinetobacter nosocomialis M2

48.844

94.795

0.463
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.533

87.945

0.436
  pilT Vibrio cholerae strain A1552

49.533

87.945

0.436
  pilT Neisseria meningitidis 8013

49.838

84.658

0.422
  pilT Neisseria gonorrhoeae MS11

49.026

84.384

0.414
  pilU Pseudomonas stutzeri DSM 10701

40.947

98.356

0.403
  pilU Vibrio cholerae strain A1552

41.714

95.89

0.4
  pilU Acinetobacter baylyi ADP1

41.667

95.342

0.397