Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   G163CM_RS21895 Genome accession   NZ_CP087880
Coordinates   4682041..4683021 (+) Length   326 a.a.
NCBI ID   WP_231826278.1    Uniprot ID   -
Organism   Pseudocitrobacter corydidari isolate G163CM     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4677041..4688021
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G163CM_RS21865 (G163CM_43630) ansB 4677632..4678678 (+) 1047 WP_231826273.1 L-asparaginase 2 -
  G163CM_RS21870 (G163CM_43640) hemW 4678717..4679853 (-) 1137 WP_231826274.1 radical SAM family heme chaperone HemW -
  G163CM_RS21875 (G163CM_43650) - 4679846..4680439 (-) 594 WP_231826275.1 XTP/dITP diphosphatase -
  G163CM_RS21880 (G163CM_43660) yggU 4680448..4680738 (-) 291 WP_231826276.1 DUF167 family protein YggU -
  G163CM_RS21885 (G163CM_43670) - 4680735..4681301 (-) 567 WP_015963064.1 YggT family protein -
  G163CM_RS21890 (G163CM_43680) - 4681310..4682023 (-) 714 WP_231826277.1 YggS family pyridoxal phosphate-dependent enzyme -
  G163CM_RS21895 (G163CM_43690) pilT 4682041..4683021 (+) 981 WP_231826278.1 type IV pilus twitching motility protein PilT Machinery gene
  G163CM_RS21900 (G163CM_43700) - 4683507..4684160 (+) 654 WP_015963067.1 LuxR C-terminal-related transcriptional regulator -
  G163CM_RS21905 (G163CM_43710) ruvX 4684256..4684678 (-) 423 WP_189623856.1 Holliday junction resolvase RuvX -
  G163CM_RS21910 (G163CM_43720) - 4684678..4685241 (-) 564 WP_231826279.1 YqgE/AlgH family protein -
  G163CM_RS21915 (G163CM_43730) gshB 4685386..4686333 (-) 948 WP_015963070.1 glutathione synthase -
  G163CM_RS21920 (G163CM_43740) rsmE 4686357..4687088 (-) 732 WP_189623858.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  G163CM_RS21925 (G163CM_43750) endA 4687223..4687930 (-) 708 WP_231826280.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35163.33 Da        Isoelectric Point: 6.9176

>NTDB_id=631980 G163CM_RS21895 WP_231826278.1 4682041..4683021(+) (pilT) [Pseudocitrobacter corydidari isolate G163CM]
MEIQEIVALSVKHNVSDLHLCSGAMARWRRCGLLESAPFSSPEPEALLRGWLSEAQHAQWRAQGQLDFAIAPSEGPRLRA
SAFRHARGVSLALRVLPTRCPTLAELGVPAALPELLHEEGGLILVTGATGSGKSTTLAAMVDYLNNTLDGHILTLEDPVE
FIHSSQRCLIQQREIGQHISSFAEALRGALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDV
FPAQEKDPVRSQLANSLCAVVAQKLVADKQGGRVALFELLVNTPAVGNLIREGKTHQLAGVMQTGQHAGMQTFSQSYQQC
VAKGKL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=631980 G163CM_RS21895 WP_231826278.1 4682041..4683021(+) (pilT) [Pseudocitrobacter corydidari isolate G163CM]
ATGGAAATACAAGAAATCGTGGCGCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGTAGCGGGGCAATGGCGCG
ATGGCGACGCTGTGGCTTGCTCGAATCTGCCCCCTTTTCATCACCCGAGCCGGAAGCATTGCTGCGCGGTTGGTTAAGCG
AGGCACAGCACGCGCAGTGGCGGGCGCAGGGCCAGCTTGATTTCGCGATAGCGCCCTCTGAAGGGCCACGCCTGCGCGCC
AGCGCATTTCGCCATGCGCGCGGGGTTTCACTGGCATTGCGTGTGCTACCGACGCGCTGCCCAACGCTCGCTGAGCTCGG
CGTGCCAGCGGCGCTACCTGAACTGTTGCATGAAGAGGGTGGGTTGATACTGGTCACCGGCGCAACCGGCAGCGGTAAGT
CAACGACGCTTGCCGCAATGGTCGATTACCTCAATAACACCCTGGATGGGCATATTCTGACGCTGGAAGATCCGGTGGAA
TTTATTCATTCCAGCCAGCGCTGCTTAATTCAACAACGCGAAATCGGTCAACATATCTCGTCATTCGCTGAGGCACTGCG
CGGCGCGCTACGTCAGGACCCCGACGTTATCCTGCTGGGGGAGTTACGCGACAGCGAGACGATCCGGCTGGCATTAACCG
CCGCCGAAACAGGCCATCTGGTGCTGGCGACCTTGCATACGCGCGGCGCGGCACAGGCGATTGAGCGTCTGGTTGACGTT
TTTCCGGCGCAGGAAAAAGATCCGGTTCGCAGCCAACTGGCGAACAGTTTGTGTGCCGTGGTGGCGCAAAAGCTGGTGGC
GGATAAACAAGGCGGGCGCGTGGCGCTCTTTGAGCTGCTGGTGAATACCCCGGCGGTAGGAAACCTGATCCGTGAGGGGA
AAACGCATCAGCTTGCAGGTGTCATGCAAACTGGACAGCACGCGGGGATGCAAACTTTTTCTCAGAGCTATCAACAATGT
GTGGCGAAGGGTAAGCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.303

100

0.509
  pilT Vibrio cholerae strain A1552

50.303

100

0.509
  pilT Neisseria meningitidis 8013

49.245

100

0.5
  pilT Neisseria gonorrhoeae MS11

48.943

100

0.497
  pilT Pseudomonas aeruginosa PAK

48.485

100

0.491
  pilT Pseudomonas stutzeri DSM 10701

48.182

100

0.488
  pilT Acinetobacter baylyi ADP1

47.879

100

0.485
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Legionella pneumophila strain Lp02

46.483

100

0.466
  pilT Legionella pneumophila strain ERS1305867

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.969

97.546

0.439
  pilU Vibrio cholerae strain A1552

41.641

100

0.42
  pilU Pseudomonas stutzeri DSM 10701

40.303

100

0.408
  pilU Acinetobacter baylyi ADP1

37.461

99.08

0.371