Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LP114_RS01375 Genome accession   NZ_CP087848
Coordinates   279054..280100 (+) Length   348 a.a.
NCBI ID   WP_029102986.1    Uniprot ID   A0A378QXY5
Organism   Moraxella bovis strain SAM57994     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 274054..285100
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LP114_RS01350 (LP114_01340) - 274774..275853 (-) 1080 WP_115368990.1 zinc-dependent alcohol dehydrogenase family protein -
  LP114_RS01355 (LP114_01345) - 276295..276942 (-) 648 WP_112741748.1 UPF0149 family protein -
  LP114_RS01360 (LP114_01350) - 277090..277455 (+) 366 WP_062499973.1 hypothetical protein -
  LP114_RS01365 (LP114_01355) - 277476..278096 (+) 621 WP_264677709.1 cell division protein ZapA -
  LP114_RS01370 (LP114_01360) - 278197..278901 (-) 705 WP_264677710.1 YggS family pyridoxal phosphate-dependent enzyme -
  LP114_RS01375 (LP114_01365) pilT 279054..280100 (+) 1047 WP_029102986.1 type IV pilus twitching motility protein PilT Machinery gene
  LP114_RS01380 (LP114_01370) pilU 280110..281234 (+) 1125 WP_078274516.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LP114_RS01385 (LP114_01375) fur 281285..281731 (-) 447 WP_264698326.1 ferric iron uptake transcriptional regulator -
  LP114_RS01390 (LP114_01380) - 281940..282827 (-) 888 WP_264677712.1 class II glutamine amidotransferase -
  LP114_RS01395 (LP114_01385) - 282829..283515 (-) 687 WP_264677713.1 hypothetical protein -
  LP114_RS14680 - 283915..284040 (+) 126 Protein_271 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38471.35 Da        Isoelectric Point: 6.9913

>NTDB_id=631806 LP114_RS01375 WP_029102986.1 279054..280100(+) (pilT) [Moraxella bovis strain SAM57994]
MSAPSIEDLLRFVVKNKASDLHLSAGLPPMIRVDGEVVRINTPEMDHSTVHKLIYDIMNDKQRADYEEFLETDFSFEIPG
LARFRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGPVFKKVSDFKRGVVLVTGPTGSGKSTTLAAMINYINETRKEHILT
IEDPIEFVHESKKSLVNQREVHRDTLGFDAALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTNSAAKTI
DRVIDVFPAAEKDMIRAMLSESLQAVISQALLKKVGGGRVAAHEIMLGTPAIRNLIRENKIAQMYSAIQTGAGEGMITLD
QTLKNLVSSRTITKETARVYAKQPEAFL

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=631806 LP114_RS01375 WP_029102986.1 279054..280100(+) (pilT) [Moraxella bovis strain SAM57994]
ATGTCAGCTCCTAGTATTGAAGACCTACTGCGTTTTGTGGTAAAAAATAAAGCGTCCGACTTGCACTTATCGGCAGGACT
ACCGCCCATGATTCGTGTGGACGGTGAAGTGGTGCGTATCAACACCCCCGAGATGGATCACTCTACTGTGCATAAGCTCA
TTTATGACATCATGAATGATAAACAACGTGCCGACTACGAAGAGTTTTTAGAAACTGACTTCTCGTTTGAGATCCCAGGT
CTGGCTCGTTTTCGTGTGAACGCCTTTAATCAAAACCGTGGTGCTGGTGCGGTATTTCGTACCATTCCATCCAAGGTTCT
GACCATGGAAGATTTGGGCATGGGTCCTGTCTTTAAAAAGGTCTCCGACTTTAAACGTGGTGTGGTACTGGTTACAGGTC
CGACAGGTTCGGGTAAATCGACCACGCTCGCTGCGATGATTAACTACATTAATGAGACCCGTAAAGAGCATATCCTAACC
ATCGAAGACCCCATCGAGTTTGTCCACGAGTCCAAAAAATCGCTCGTCAACCAACGTGAAGTACACCGTGACACCCTAGG
CTTTGATGCTGCCTTGCGTTCGGCACTGCGTGAAGACCCTGACATCATTTTGGTCGGTGAGATGCGTGACTTAGAGACCA
TCCGTCTTGCTCTAACCGCTGCCGAGACAGGACACTTGGTGTTTGGGACACTCCACACCAACTCTGCTGCCAAGACCATT
GACCGTGTGATTGACGTATTCCCTGCCGCCGAAAAAGACATGATTCGTGCGATGTTATCCGAGTCCCTGCAAGCGGTTAT
CTCGCAGGCCCTACTCAAAAAAGTCGGGGGCGGTCGTGTGGCAGCTCATGAAATCATGCTTGGCACGCCTGCCATTCGCA
ACCTTATCCGTGAAAACAAAATCGCCCAAATGTACTCTGCCATCCAAACAGGGGCAGGCGAAGGCATGATTACCCTTGAC
CAGACCTTAAAGAACTTGGTCTCATCTCGCACCATCACTAAAGAGACGGCACGTGTCTATGCCAAACAGCCAGAAGCGTT
CTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A378QXY5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

81.633

98.563

0.805
  pilT Acinetobacter baumannii D1279779

81.341

98.563

0.802
  pilT Acinetobacter baumannii strain A118

81.341

98.563

0.802
  pilT Pseudomonas stutzeri DSM 10701

80.409

98.276

0.79
  pilT Pseudomonas aeruginosa PAK

80.409

98.276

0.79
  pilT Acinetobacter baylyi ADP1

79.24

98.276

0.779
  pilT Legionella pneumophila strain Lp02

73.392

98.276

0.721
  pilT Legionella pneumophila strain ERS1305867

73.392

98.276

0.721
  pilT Neisseria meningitidis 8013

66.472

98.563

0.655
  pilT Neisseria gonorrhoeae MS11

66.181

98.563

0.652
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.35

92.816

0.644
  pilT Vibrio cholerae strain A1552

69.35

92.816

0.644
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.478

98.563

0.517
  pilU Pseudomonas stutzeri DSM 10701

42.522

97.989

0.417
  pilU Acinetobacter baylyi ADP1

40.708

97.414

0.397
  pilU Vibrio cholerae strain A1552

41.411

93.678

0.388