Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N5861_RS17860 Genome accession   NZ_CP104598
Coordinates   3695995..3696975 (-) Length   326 a.a.
NCBI ID   WP_250308302.1    Uniprot ID   -
Organism   Escherichia coli strain AHM8C160RI     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3690995..3701975
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5861_RS17830 (N5861_17765) yggI 3691658..3692155 (+) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -
  N5861_RS17835 (N5861_17770) endA 3692250..3692957 (+) 708 WP_000286500.1 deoxyribonuclease I -
  N5861_RS17840 (N5861_17775) rsmE 3693037..3693768 (+) 732 WP_001222508.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  N5861_RS17845 (N5861_17780) gshB 3693781..3694731 (+) 951 WP_000593273.1 glutathione synthase -
  N5861_RS17850 (N5861_17785) yqgE 3694840..3695403 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  N5861_RS17855 (N5861_17790) ruvX 3695403..3695819 (+) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  N5861_RS17860 (N5861_17795) pilT 3695995..3696975 (-) 981 WP_250308302.1 type IV pilus twitching motility protein PilT Machinery gene
  N5861_RS17865 (N5861_17800) yggS 3696993..3697697 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  N5861_RS17870 (N5861_17805) yggT 3697715..3698281 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  N5861_RS17875 (N5861_17810) yggU 3698278..3698568 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  N5861_RS17880 (N5861_17815) rdgB 3698576..3699169 (+) 594 WP_001174745.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  N5861_RS17885 (N5861_17820) hemW 3699162..3700298 (+) 1137 WP_097702201.1 radical SAM family heme chaperone HemW -
  N5861_RS17890 (N5861_17825) - 3700612..3701598 (+) 987 WP_077039125.1 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35951.13 Da        Isoelectric Point: 5.9549

>NTDB_id=631660 N5861_RS17860 WP_250308302.1 3695995..3696975(-) (pilT) [Escherichia coli strain AHM8C160RI]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYHQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=631660 N5861_RS17860 WP_250308302.1 3695995..3696975(-) (pilT) [Escherichia coli strain AHM8C160RI]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACTTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCACCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365