Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LP109_RS12370 Genome accession   NZ_CP087817
Coordinates   2446322..2447446 (-) Length   374 a.a.
NCBI ID   WP_078274516.1    Uniprot ID   A0A1S9ZZQ6
Organism   Moraxella bovis strain SAM109237     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2441322..2452446
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LP109_RS12330 (LP109_12215) - 2441841..2442383 (-) 543 WP_115369001.1 hypothetical protein -
  LP109_RS12335 (LP109_12220) - 2442512..2442919 (-) 408 WP_264676512.1 hypothetical protein -
  LP109_RS12340 (LP109_12225) - 2442936..2443358 (-) 423 WP_264676513.1 hypothetical protein -
  LP109_RS12345 (LP109_12230) - 2443520..2443645 (-) 126 Protein_2406 helix-turn-helix domain-containing protein -
  LP109_RS12350 (LP109_12235) - 2443971..2444249 (+) 279 WP_264676514.1 hypothetical protein -
  LP109_RS12355 (LP109_12240) - 2444325..2444729 (+) 405 WP_264676515.1 hypothetical protein -
  LP109_RS12360 (LP109_12245) - 2444731..2445618 (+) 888 WP_078274515.1 class II glutamine amidotransferase -
  LP109_RS12365 (LP109_12250) fur 2445826..2446272 (+) 447 WP_029102984.1 ferric iron uptake transcriptional regulator -
  LP109_RS12370 (LP109_12255) pilU 2446322..2447446 (-) 1125 WP_078274516.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LP109_RS12375 (LP109_12260) pilT 2447456..2448502 (-) 1047 WP_078274517.1 type IV pilus twitching motility protein PilT Machinery gene
  LP109_RS12380 (LP109_12265) - 2448655..2449359 (+) 705 WP_078274518.1 YggS family pyridoxal phosphate-dependent enzyme -
  LP109_RS12385 (LP109_12270) - 2449460..2450089 (-) 630 WP_078274519.1 cell division protein ZapA -
  LP109_RS12390 (LP109_12275) - 2450110..2450475 (-) 366 WP_078274520.1 hypothetical protein -
  LP109_RS12395 (LP109_12280) - 2450623..2451270 (+) 648 WP_264676516.1 UPF0149 family protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 41843.19 Da        Isoelectric Point: 6.4355

>NTDB_id=631625 LP109_RS12370 WP_078274516.1 2446322..2447446(-) (pilU) [Moraxella bovis strain SAM109237]
MDFDKLLQVMIAKNGSDLFITEGVPPSLKVNGTILPMTRTPLTAEQTMALVTSIMTEAQKKEFHETNECQFAISDKAEAA
RFRVSAMIQRNKAAMVLRKIETQIPTTEDLNLPPILKELAMKKRGIILLVGATGTGKSTTLAAMIGHRNENSRGHIITIE
DPIEYVHQHKGCIVTQREVGIDTLSFEEGLKNTLRQAPDVILIGEIRNREVMSYAIQYAETGHLVFATLHANNANQAIDR
IIHFFEADRHGQLFMDLSLNLRAIIAQQLIPTPDGKGRRAAIEILLGTQLIADYIRKGEIHEIKPVMKRSRDIGMQTFDQ
ALFDLYESGQITYQDALKHADSPNDLRLQIKLESKNAVVEEESAKLSIDMGDYN

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=631625 LP109_RS12370 WP_078274516.1 2446322..2447446(-) (pilU) [Moraxella bovis strain SAM109237]
ATGGATTTTGATAAACTACTACAAGTGATGATTGCCAAAAACGGCTCTGACCTTTTTATCACCGAAGGCGTTCCCCCATC
ACTCAAAGTCAATGGCACCATTTTGCCCATGACCAGAACGCCACTAACCGCCGAGCAGACCATGGCACTGGTAACCAGCA
TCATGACCGAAGCTCAGAAAAAAGAGTTTCATGAGACCAACGAGTGCCAATTTGCCATCTCTGACAAAGCCGAAGCAGCT
CGTTTTCGTGTGTCGGCGATGATTCAGCGCAACAAGGCAGCGATGGTACTGCGTAAGATTGAGACACAAATCCCCACCAC
CGAAGATTTGAACCTACCACCCATCCTAAAAGAGCTTGCCATGAAAAAGCGTGGCATTATCCTACTTGTGGGTGCGACAG
GTACGGGTAAATCCACCACGCTGGCTGCCATGATTGGCCATCGTAACGAGAACTCTCGTGGTCATATCATCACTATCGAA
GACCCGATTGAATACGTTCATCAGCACAAAGGCTGTATCGTCACTCAGCGTGAGGTGGGTATTGACACGCTCTCGTTTGA
AGAAGGTCTAAAAAACACTCTACGTCAAGCCCCTGATGTCATCCTTATCGGTGAGATTCGTAACCGTGAAGTCATGAGCT
ATGCCATCCAGTACGCTGAGACGGGTCACTTGGTGTTTGCCACGCTCCACGCCAACAACGCCAACCAAGCCATCGACCGT
ATCATTCACTTCTTTGAAGCGGACAGACACGGACAGTTATTCATGGACTTGTCACTTAACTTGCGTGCCATCATCGCCCA
ACAGCTTATCCCAACCCCTGATGGCAAAGGTCGCCGTGCCGCCATTGAGATTTTATTGGGTACACAGCTCATCGCTGACT
ACATTCGCAAGGGCGAGATTCACGAGATTAAACCTGTGATGAAACGCTCTCGTGACATCGGTATGCAGACCTTTGACCAA
GCGTTGTTCGACCTGTATGAATCAGGACAAATCACCTATCAAGACGCACTCAAACACGCTGATAGCCCGAACGACCTACG
TCTACAAATCAAACTCGAAAGCAAAAATGCTGTGGTCGAAGAAGAGTCTGCCAAACTCTCTATTGACATGGGCGATTATA
ATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(40-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1S9ZZQ6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.845

100

0.668
  pilU Acinetobacter baylyi ADP1

67.898

94.118

0.639
  pilU Vibrio cholerae strain A1552

52.162

98.93

0.516
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.816

93.048

0.398
  pilT Legionella pneumophila strain Lp02

43.452

89.84

0.39
  pilT Legionella pneumophila strain ERS1305867

43.452

89.84

0.39
  pilT Acinetobacter baumannii D1279779

40.816

91.711

0.374
  pilT Acinetobacter nosocomialis M2

40.816

91.711

0.374
  pilT Acinetobacter baumannii strain A118

40.816

91.711

0.374
  pilT Pseudomonas aeruginosa PAK

40.173

92.513

0.372
  pilT Neisseria meningitidis 8013

40.762

91.176

0.372
  pilT Pseudomonas stutzeri DSM 10701

39.595

92.513

0.366
  pilT Neisseria gonorrhoeae MS11

40

90.909

0.364
  pilT Vibrio cholerae strain A1552

40.597

89.572

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.597

89.572

0.364
  pilT Acinetobacter baylyi ADP1

39.359

91.711

0.361