Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LP127_RS01725 Genome accession   NZ_CP087771
Coordinates   350336..351382 (+) Length   348 a.a.
NCBI ID   WP_078274517.1    Uniprot ID   A0A1S9ZZL3
Organism   Moraxella bovis strain SAM109244     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 345336..356382
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LP127_RS01700 (LP127_01695) - 346047..347126 (-) 1080 WP_115368990.1 zinc-dependent alcohol dehydrogenase family protein -
  LP127_RS01705 (LP127_01700) - 347568..348215 (-) 648 WP_112741748.1 UPF0149 family protein -
  LP127_RS01710 (LP127_01705) - 348363..348728 (+) 366 WP_078274520.1 hypothetical protein -
  LP127_RS01715 (LP127_01710) - 348749..349378 (+) 630 WP_078274519.1 cell division protein ZapA -
  LP127_RS01720 (LP127_01715) - 349479..350183 (-) 705 WP_112741749.1 YggS family pyridoxal phosphate-dependent enzyme -
  LP127_RS01725 (LP127_01720) pilT 350336..351382 (+) 1047 WP_078274517.1 type IV pilus twitching motility protein PilT Machinery gene
  LP127_RS01730 (LP127_01725) pilU 351392..352516 (+) 1125 WP_078274516.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LP127_RS01735 (LP127_01730) fur 352566..353012 (-) 447 WP_029102984.1 ferric iron uptake transcriptional regulator -
  LP127_RS01740 (LP127_01735) - 353220..354107 (-) 888 WP_078274515.1 class II glutamine amidotransferase -
  LP127_RS01745 (LP127_01740) - 354109..354513 (-) 405 WP_078274514.1 hypothetical protein -
  LP127_RS01750 (LP127_01745) - 354589..354867 (-) 279 WP_158079665.1 hypothetical protein -
  LP127_RS01755 (LP127_01750) - 355193..355318 (+) 126 Protein_341 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38455.35 Da        Isoelectric Point: 6.9913

>NTDB_id=631363 LP127_RS01725 WP_078274517.1 350336..351382(+) (pilT) [Moraxella bovis strain SAM109244]
MSAPSIEDLLRFVVKNKASDLHLSAGLPPMIRVDGEVVRINTPEMDHSTVHKLIYDIMNDKQRADYEEFLETDFSFEIPG
LARFRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGPVFKKVSDFKRGVVLVTGPTGSGKSTTLAAMINYINETRKEHILT
IEDPIEFVHESKKSLVNQREVHRDTLGFDAALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTNSAAKTI
DRVIDVFPAAEKDMIRAMLSESLQAVISQALLKKVGGGRVAAHEIMLGTPAIRNLIRENKIAQMYSAIQTGAGEGMITLD
QTLKNLVASRTITKETARVYAKQPEAFL

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=631363 LP127_RS01725 WP_078274517.1 350336..351382(+) (pilT) [Moraxella bovis strain SAM109244]
ATGTCAGCTCCTAGTATTGAAGACCTACTGCGTTTTGTGGTAAAAAATAAAGCGTCCGACTTGCACTTATCGGCAGGACT
ACCGCCCATGATTCGTGTGGACGGTGAAGTGGTGCGTATCAACACCCCCGAGATGGATCACTCTACTGTGCATAAGCTCA
TTTATGACATCATGAATGATAAACAACGTGCCGACTACGAAGAGTTTTTAGAAACTGACTTCTCGTTTGAAATCCCAGGT
CTGGCTCGTTTTCGTGTGAACGCCTTTAACCAAAACCGTGGTGCTGGTGCGGTATTTCGTACCATTCCATCCAAGGTTCT
GACCATGGAAGATTTGGGCATGGGTCCTGTCTTTAAAAAGGTCTCCGACTTTAAACGTGGTGTGGTACTGGTTACAGGTC
CGACAGGTTCGGGTAAATCGACCACGCTCGCTGCGATGATTAACTACATTAATGAGACCCGTAAAGAGCATATCCTAACC
ATCGAAGACCCCATCGAGTTTGTCCACGAGTCCAAAAAATCGCTCGTCAACCAACGTGAAGTACACCGTGATACCCTAGG
CTTTGATGCTGCCTTGCGTTCGGCACTGCGTGAAGACCCTGACATCATTTTGGTCGGTGAGATGCGTGACTTAGAGACCA
TCCGTCTTGCCCTAACCGCTGCCGAGACAGGGCACTTGGTGTTTGGGACACTCCACACCAACTCTGCTGCCAAGACCATT
GACCGTGTGATTGACGTATTCCCTGCCGCCGAAAAAGACATGATTCGTGCGATGTTATCCGAGTCCTTGCAGGCGGTTAT
CTCGCAAGCCCTACTCAAAAAAGTCGGGGGCGGTCGTGTGGCAGCTCACGAGATTATGCTTGGTACGCCTGCCATTCGTA
ACCTTATCCGTGAAAACAAAATCGCCCAAATGTACTCTGCCATCCAAACAGGGGCAGGCGAAGGCATGATTACCCTTGAC
CAGACCCTAAAGAACTTGGTCGCATCTCGCACCATTACCAAAGAGACGGCACGTGTCTATGCCAAACAGCCAGAAGCGTT
CTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1S9ZZL3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

81.633

98.563

0.805
  pilT Acinetobacter baumannii strain A118

81.633

98.563

0.805
  pilT Acinetobacter nosocomialis M2

81.341

98.563

0.802
  pilT Pseudomonas stutzeri DSM 10701

80.702

98.276

0.793
  pilT Pseudomonas aeruginosa PAK

80.702

98.276

0.793
  pilT Acinetobacter baylyi ADP1

79.532

98.276

0.782
  pilT Legionella pneumophila strain Lp02

73.392

98.276

0.721
  pilT Legionella pneumophila strain ERS1305867

73.392

98.276

0.721
  pilT Neisseria meningitidis 8013

66.472

98.563

0.655
  pilT Neisseria gonorrhoeae MS11

66.181

98.563

0.652
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.659

92.816

0.647
  pilT Vibrio cholerae strain A1552

69.659

92.816

0.647
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.161

99.713

0.52
  pilU Pseudomonas stutzeri DSM 10701

42.522

97.989

0.417
  pilU Acinetobacter baylyi ADP1

40.708

97.414

0.397
  pilU Vibrio cholerae strain A1552

41.411

93.678

0.388