Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N4P55_RS26910 Genome accession   NZ_CP104408
Coordinates   5875461..5876885 (-) Length   474 a.a.
NCBI ID   WP_262666482.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain Ant01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5870461..5881885
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4P55_RS26880 (N4P55_26895) thiS 5871146..5871346 (-) 201 WP_053258809.1 sulfur carrier protein ThiS -
  N4P55_RS26885 (N4P55_26900) - 5871426..5871800 (-) 375 WP_064454557.1 DUF423 domain-containing protein -
  N4P55_RS26890 (N4P55_26905) mtgA 5871875..5872597 (+) 723 WP_064454558.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  N4P55_RS26895 (N4P55_26910) rpoH 5872790..5873644 (-) 855 WP_003176698.1 RNA polymerase sigma factor RpoH -
  N4P55_RS26900 (N4P55_26915) ftsX 5873774..5874796 (-) 1023 WP_064454559.1 permease-like cell division protein FtsX -
  N4P55_RS26905 (N4P55_26920) ftsE 5874793..5875464 (-) 672 WP_003213784.1 cell division ATP-binding protein FtsE -
  N4P55_RS26910 (N4P55_26925) pilA 5875461..5876885 (-) 1425 WP_262666482.1 signal recognition particle-docking protein FtsY Machinery gene
  N4P55_RS26915 (N4P55_26930) - 5877224..5878579 (+) 1356 WP_064454561.1 M16 family metallopeptidase -
  N4P55_RS26920 (N4P55_26935) - 5878572..5880062 (+) 1491 WP_064454562.1 M16 family metallopeptidase -
  N4P55_RS26925 (N4P55_26940) rsmD 5880062..5880679 (+) 618 WP_064454563.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  N4P55_RS26930 (N4P55_26945) - 5880740..5881738 (+) 999 WP_262666483.1 hydrolase -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 50462.42 Da        Isoelectric Point: 4.6805

>NTDB_id=629362 N4P55_RS26910 WP_262666482.1 5875461..5876885(-) (pilA) [Pseudomonas fluorescens strain Ant01]
MFGSNDDKKSPAAAGEKKGLFGWLRKKPQETVVEQSQVQPEPIPEPVLEAEPVAETPAPIVLPMAEPVLQPVEQVEPEPE
HKPWLELPVAEEPVALVEDVQAEHVTPPIPVVVEPVAVVVEAPPAIEASALVVVEPTPAPVVVAAPAVIEPPVAPEPVVV
PAETSKTGFFARLKQGLSKTSASIGEGMASLFLGKKVIDDELLEDIETRLLTADVGVEATAVIIQSLTQKVARKQLTDAD
ALYKSLQAELAAMLKPVEAPLVITPNKPFVILVVGVNGAGKTTTIGKLAKKLQLEGKKVMLAAGDTFRAAAVEQLQVWGE
RNKIPVIAQHTGADSASVIFDAVQAAKARNIDVLIADTAGRLHTKDNLMEELKKVRRVISKLDAEAPHEVLLVLDAGTGQ
NAISQAKQFNQTVQLTGLALTKLDGTAKGGVIFALAKQFGLPIRYIGVGEGIDDLRTFEAEPFVQALFAERERS

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=629362 N4P55_RS26910 WP_262666482.1 5875461..5876885(-) (pilA) [Pseudomonas fluorescens strain Ant01]
ATGTTTGGTTCCAACGACGACAAGAAGAGCCCAGCTGCGGCTGGCGAGAAAAAAGGCCTGTTCGGATGGCTGCGCAAAAA
GCCGCAGGAAACCGTCGTCGAACAGTCGCAGGTTCAACCTGAGCCTATCCCAGAGCCTGTACTGGAAGCCGAACCGGTCG
CTGAAACCCCGGCGCCGATCGTGTTGCCGATGGCTGAGCCGGTATTGCAGCCAGTGGAACAGGTTGAGCCAGAGCCAGAG
CACAAACCGTGGCTGGAACTGCCGGTGGCCGAAGAGCCCGTGGCCTTGGTCGAAGATGTGCAGGCTGAGCACGTGACGCC
GCCGATTCCAGTCGTGGTTGAGCCGGTAGCGGTTGTGGTCGAAGCGCCGCCAGCCATCGAAGCATCTGCGCTGGTAGTTG
TAGAGCCAACACCGGCCCCCGTGGTTGTTGCCGCGCCTGCCGTTATCGAGCCGCCGGTCGCACCAGAGCCGGTCGTCGTT
CCCGCCGAAACCAGCAAGACCGGCTTCTTCGCCCGTCTCAAGCAAGGCCTGAGCAAGACCAGCGCCAGCATCGGCGAAGG
TATGGCCAGCCTGTTCCTGGGCAAGAAGGTCATTGATGATGAACTGCTGGAAGACATCGAAACTCGCCTGCTGACTGCCG
ACGTGGGCGTCGAAGCCACTGCGGTGATCATCCAGAGCCTGACCCAGAAGGTCGCGCGCAAGCAGTTGACCGACGCCGAT
GCGCTTTACAAATCCCTGCAGGCCGAGCTGGCTGCGATGCTCAAACCCGTCGAAGCCCCGCTGGTGATCACGCCGAACAA
ACCGTTCGTGATCCTGGTGGTCGGCGTCAACGGCGCCGGCAAAACCACCACCATCGGCAAGCTGGCCAAGAAACTGCAGT
TGGAAGGCAAGAAAGTCATGCTTGCCGCCGGTGACACATTCCGTGCCGCCGCCGTGGAGCAGCTGCAAGTCTGGGGTGAG
CGCAACAAGATTCCGGTGATCGCCCAGCACACCGGCGCCGACTCCGCCTCGGTGATCTTCGATGCCGTGCAAGCCGCCAA
GGCCCGTAACATCGATGTGTTGATCGCCGATACCGCCGGTCGCCTGCACACCAAAGACAACTTGATGGAAGAGCTGAAGA
AAGTGCGCCGCGTGATCAGCAAGCTCGACGCCGAAGCACCGCACGAAGTGTTGCTGGTGCTCGACGCCGGCACCGGCCAG
AACGCCATCAGCCAGGCCAAACAATTCAACCAGACGGTGCAACTGACCGGCCTGGCATTGACTAAGCTCGACGGCACGGC
CAAGGGCGGCGTGATCTTCGCCCTGGCCAAACAGTTCGGGTTGCCGATTCGTTATATCGGCGTCGGTGAAGGCATCGACG
ACCTGCGCACCTTTGAAGCCGAACCCTTTGTACAGGCACTTTTCGCCGAGCGGGAGCGTTCATGA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(185-250)

SRP54-type protein, GTPase domain (SRP54)

(268-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

46.137

95.57

0.441