Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LO739_RS18855 Genome accession   NZ_CP087280
Coordinates   3977195..3978172 (-) Length   325 a.a.
NCBI ID   WP_032613932.1    Uniprot ID   -
Organism   Leclercia adecarboxylata strain SH19PE29     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3972195..3983172
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LO739_RS18825 (LO739_18820) endA 3972781..3973488 (+) 708 WP_032613924.1 deoxyribonuclease I -
  LO739_RS18830 (LO739_18825) rsmE 3973600..3974331 (+) 732 WP_032613955.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LO739_RS18835 (LO739_18830) gshB 3974351..3975298 (+) 948 WP_032613926.1 glutathione synthase -
  LO739_RS18840 (LO739_18835) - 3975390..3975950 (+) 561 WP_032613928.1 YqgE/AlgH family protein -
  LO739_RS18845 (LO739_18840) ruvX 3975950..3976366 (+) 417 WP_032613930.1 Holliday junction resolvase RuvX -
  LO739_RS18850 (LO739_18845) - 3976406..3977095 (-) 690 WP_032613957.1 hypothetical protein -
  LO739_RS18855 (LO739_18850) pilT 3977195..3978172 (-) 978 WP_032613932.1 type IV pilus twitching motility protein PilT Machinery gene
  LO739_RS18860 (LO739_18855) - 3978190..3978891 (+) 702 WP_032613935.1 YggS family pyridoxal phosphate-dependent enzyme -
  LO739_RS18865 (LO739_18860) - 3978913..3979479 (+) 567 WP_032613937.1 YggT family protein -
  LO739_RS18870 (LO739_18865) yggU 3979476..3979772 (+) 297 WP_032613939.1 DUF167 family protein YggU -
  LO739_RS18875 (LO739_18870) - 3979776..3980369 (+) 594 WP_059307228.1 XTP/dITP diphosphatase -
  LO739_RS18880 (LO739_18875) hemW 3980362..3981504 (+) 1143 WP_032613943.1 radical SAM family heme chaperone HemW -
  LO739_RS18885 (LO739_18880) - 3981565..3981927 (+) 363 WP_032613945.1 endonuclease domain-containing protein -
  LO739_RS18890 (LO739_18885) - 3982221..3982940 (-) 720 WP_103792620.1 DUF2884 domain-containing protein -

Sequence

Protein


Download         Length: 325 a.a.        Molecular weight: 35677.89 Da        Isoelectric Point: 6.8724

>NTDB_id=628315 LO739_RS18855 WP_032613932.1 3977195..3978172(-) (pilT) [Leclercia adecarboxylata strain SH19PE29]
MNMEEIVALSVKHNVSDLHLCSNAPPRWRRRGKMELAPFPGPDVVMLLKSWLSDEQQGAWCAQGQVDFAVTLESHRLRAS
AFAHTQGCSLALRLLPEQCPQLRTLGTPQAIPELLTRDNGLILVTGATGSGKSTTLAAMVDFLNHHSDGHILTLEDPVEF
IHQSKRCLVQQREIGLHCNAFADALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDAF
PAEEKEPVRKQLAGSLQAVLAQKLERDNQQGRVALFELLINTPAAANLIREGKTYQLPGVIQTGLQAGMQNFEQSRAERK
AQGRL

Nucleotide


Download         Length: 978 bp        

>NTDB_id=628315 LO739_RS18855 WP_032613932.1 3977195..3978172(-) (pilT) [Leclercia adecarboxylata strain SH19PE29]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAGTAACGCACCACCTCG
CTGGCGACGACGAGGAAAGATGGAATTGGCGCCTTTTCCGGGACCGGATGTGGTGATGCTGCTGAAAAGCTGGCTCAGCG
ATGAACAACAGGGGGCATGGTGTGCTCAGGGGCAGGTCGATTTTGCCGTCACTCTGGAGAGCCACCGGTTGCGGGCCAGC
GCCTTCGCCCATACGCAAGGGTGTTCGCTGGCGCTCCGGCTGCTGCCGGAACAGTGTCCGCAATTGCGTACCCTAGGTAC
GCCGCAGGCGATCCCCGAACTGCTGACCAGGGATAACGGCCTGATTCTGGTGACCGGTGCCACCGGCAGCGGCAAGTCCA
CCACGCTTGCGGCAATGGTCGACTTTCTTAATCACCACAGCGACGGACATATCCTGACCCTGGAAGATCCGGTGGAGTTT
ATCCATCAGAGCAAACGTTGCCTGGTGCAGCAGCGGGAGATTGGCCTGCACTGCAACGCCTTTGCCGATGCGCTGCGGGC
GGCATTGCGGGAAGATCCCGATGTGATCCTGTTAGGCGAACTGCGCGATAGCGAAACGATCCGCCTTGCGCTTACTGCCG
CAGAGACCGGGCATCTGGTGCTGGCGACCCTGCATACCCGTGGCGCAGCCCAGGCGATAGAGCGGCTGGTGGATGCCTTC
CCGGCGGAGGAAAAAGAGCCGGTACGCAAGCAGCTGGCCGGCAGTTTGCAGGCGGTGCTGGCACAAAAACTCGAAAGGGA
CAACCAGCAGGGGCGCGTGGCGCTGTTTGAGTTACTCATTAACACTCCAGCTGCGGCGAATTTGATTCGTGAAGGCAAAA
CCTATCAACTGCCTGGCGTGATCCAGACCGGGCTGCAGGCAGGGATGCAGAACTTCGAGCAGAGCCGTGCTGAACGCAAG
GCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.489
  pilT Vibrio cholerae strain A1552

48.624

100

0.489
  pilT Legionella pneumophila strain Lp02

47.826

99.077

0.474
  pilT Legionella pneumophila strain ERS1305867

47.826

99.077

0.474
  pilT Neisseria meningitidis 8013

46.951

100

0.474
  pilT Pseudomonas stutzeri DSM 10701

48.265

97.538

0.471
  pilT Acinetobacter baylyi ADP1

46.789

100

0.471
  pilT Acinetobacter baumannii strain A118

47.649

98.154

0.468
  pilT Acinetobacter baumannii D1279779

47.649

98.154

0.468
  pilT Acinetobacter nosocomialis M2

47.649

98.154

0.468
  pilT Neisseria gonorrhoeae MS11

47.188

98.462

0.465
  pilT Pseudomonas aeruginosa PAK

47.634

97.538

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.631

96.615

0.422
  pilU Vibrio cholerae strain A1552

40.252

97.846

0.394
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.385
  pilU Acinetobacter baylyi ADP1

36.42

99.692

0.363