Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LOY68_RS29935 Genome accession   NZ_CP087205
Coordinates   6666086..6667120 (-) Length   344 a.a.
NCBI ID   WP_163910953.1    Uniprot ID   A0A6L5BLH7
Organism   Pseudomonas sp. B21-053     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6661086..6672120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY68_RS29905 (LOY68_29880) ruvX 6661245..6661682 (+) 438 WP_005792259.1 Holliday junction resolvase RuvX -
  LOY68_RS29910 (LOY68_29885) pyrR 6661764..6662270 (+) 507 WP_054044401.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  LOY68_RS29915 (LOY68_29890) - 6662297..6663301 (+) 1005 WP_007897943.1 aspartate carbamoyltransferase catalytic subunit -
  LOY68_RS29920 (LOY68_29895) - 6663298..6664569 (+) 1272 WP_265041395.1 dihydroorotase -
  LOY68_RS29925 (LOY68_29900) - 6664671..6665105 (-) 435 WP_163910960.1 NINE protein -
  LOY68_RS29930 (LOY68_29905) - 6665356..6665976 (+) 621 WP_265041396.1 C40 family peptidase -
  LOY68_RS29935 (LOY68_29910) pilT 6666086..6667120 (-) 1035 WP_163910953.1 type IV pilus twitching motility protein PilT Machinery gene
  LOY68_RS29940 (LOY68_29915) - 6667177..6667875 (+) 699 WP_265041397.1 YggS family pyridoxal phosphate-dependent enzyme -
  LOY68_RS29945 (LOY68_29920) proC 6667911..6668729 (+) 819 WP_265041398.1 pyrroline-5-carboxylate reductase -
  LOY68_RS29950 (LOY68_29925) - 6668754..6669341 (+) 588 WP_163910944.1 YggT family protein -
  LOY68_RS29955 (LOY68_29930) metX 6669539..6670678 (+) 1140 WP_054044416.1 homoserine O-succinyltransferase MetX -
  LOY68_RS29960 (LOY68_29935) metW 6670686..6671306 (+) 621 WP_054044417.1 methionine biosynthesis protein MetW -
  LOY68_RS29965 (LOY68_29940) - 6671333..6671767 (+) 435 WP_163910938.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38127.76 Da        Isoelectric Point: 7.0158

>NTDB_id=628135 LOY68_RS29935 WP_163910953.1 6666086..6667120(-) (pilT) [Pseudomonas sp. B21-053]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMDDLGMGDVFRKITEAPRGLVLVTGPTGSGKSTTLAAMIDYLNNHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRIAAHEIMLGTSAIRNLIREDKVAQMYSSIQTGGNLGMQTLDMCL
KELVTKGLISREHAREKARSPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=628135 LOY68_RS29935 WP_163910953.1 6666086..6667120(-) (pilT) [Pseudomonas sp. B21-053]
ATGGATATCACTGAGCTGCTGGCATTCAGCGCCAAACAGGGCGCGTCCGACTTGCACCTGTCTGCCGGCCTGCCCCCGAT
GATTCGCGTCGATGGCGATGTGCGGCGCATCAACCTGCCGGCCCTGGACCACAAACAGGTGCACGAGCTGATCTACGACA
TCATGAACGACACCCAGCGGGTGGACTTCGAGAAGCACCTGGAAACCGACTTCTCCTTCGAAGTGCCGGGCGTGGCGCGC
TTCCGGGTCAACGCGTTCAACCAGAATCGTGGCGCCGGCGCGGTGTTTCGGACCATTCCGTCGAAGGTCCTGAGCATGGA
CGACCTCGGCATGGGCGATGTGTTTCGCAAGATTACCGAAGCCCCCCGTGGCCTGGTGCTGGTGACGGGCCCGACCGGCT
CCGGCAAGTCCACCACCCTGGCAGCGATGATCGATTACCTGAACAACCATCGCCATCACCACATCCTCACCATCGAAGAC
CCGATCGAGTTCGTCCACGAATCACGCAAATGTCTGATCAATCAGCGCGAAGTCCACCGCGATACCCGCAGTTTCGCTAC
CGCGCTACGCTCGGCATTGCGCGAAGACCCGGATGTAATTCTGGTGGGGGAGATGCGCGATCTGGAAACCATTCGCCTGG
CGTTGACCGCAGCCGAGACCGGCCACCTGGTGTTTGGCACGCTGCACACCACGTCGGCGGCGAAGACGATTGACCGGGTG
GTAGACGTGTTTCCGGGAGATGAGAAGTCGATGGTGCGCTCGATGCTGTCCGAGTCGTTGCTGGCGGTGGTGTCGCAGAC
GCTGATCAAGAAGATTGGCGGCGGACGGATTGCGGCGCACGAGATCATGCTCGGCACGTCAGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGTCGATTCAGACCGGGGGCAATCTGGGGATGCAGACGCTGGATATGTGCTTG
AAGGAATTGGTGACCAAGGGCTTGATCAGCCGCGAGCATGCGCGGGAAAAAGCGCGGTCGCCGGATAATTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6L5BLH7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

87.5

100

0.875
  pilT Pseudomonas aeruginosa PAK

86.919

100

0.869
  pilT Acinetobacter nosocomialis M2

79.07

100

0.791
  pilT Acinetobacter baumannii D1279779

79.07

100

0.791
  pilT Acinetobacter baumannii strain A118

79.07

100

0.791
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Legionella pneumophila strain Lp02

71.802

100

0.718
  pilT Legionella pneumophila strain ERS1305867

71.802

100

0.718
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.953

97.965

0.666
  pilT Vibrio cholerae strain A1552

67.953

97.965

0.666
  pilT Neisseria meningitidis 8013

66.087

100

0.663
  pilT Neisseria gonorrhoeae MS11

65.797

100

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Acinetobacter baylyi ADP1

40.751

100

0.41
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Vibrio cholerae strain A1552

39.71

100

0.398