Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LOY67_RS26415 Genome accession   NZ_CP087203
Coordinates   6028384..6029418 (-) Length   344 a.a.
NCBI ID   WP_265065087.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-056     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6023384..6034418
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY67_RS26390 (LOY67_26365) - 6024265..6025536 (+) 1272 WP_265065082.1 dihydroorotase -
  LOY67_RS26395 (LOY67_26370) - 6025660..6026088 (-) 429 WP_265065083.1 NINE protein -
  LOY67_RS26400 (LOY67_26375) - 6026338..6026949 (+) 612 WP_265065084.1 C40 family peptidase -
  LOY67_RS26405 - 6027005..6027724 (+) 720 WP_265065085.1 IS4 family transposase -
  LOY67_RS26410 - 6027784..6028236 (+) 453 WP_265065086.1 transposase -
  LOY67_RS26415 (LOY67_26385) pilT 6028384..6029418 (-) 1035 WP_265065087.1 type IV pilus twitching motility protein PilT Machinery gene
  LOY67_RS26420 (LOY67_26390) - 6029475..6030167 (+) 693 WP_265065088.1 YggS family pyridoxal phosphate-dependent enzyme -
  LOY67_RS26425 (LOY67_26395) proC 6030204..6031022 (+) 819 WP_265065089.1 pyrroline-5-carboxylate reductase -
  LOY67_RS26430 (LOY67_26400) - 6031033..6031623 (+) 591 WP_265065090.1 YggT family protein -
  LOY67_RS26435 (LOY67_26405) - 6031685..6032854 (-) 1170 WP_265064268.1 IS4 family transposase -
  LOY67_RS26440 (LOY67_26410) metX 6033132..6034271 (+) 1140 WP_265065091.1 homoserine O-succinyltransferase MetX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38122.78 Da        Isoelectric Point: 6.9777

>NTDB_id=628076 LOY67_RS26415 WP_265065087.1 6028384..6029418(-) (pilT) [Pseudomonas sp. B21-056]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDRQRVDYEKFLETDFSFDVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKVTEAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHKHHHILTIED
PIEFVHEPRKCLINQREVHRDTQGFSTALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLQAVISQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLVTKGLISREHARERARTPDSF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=628076 LOY67_RS26415 WP_265065087.1 6028384..6029418(-) (pilT) [Pseudomonas sp. B21-056]
ATGGATATCACTGAACTGTTGGCGTTCAGCGCCAAACAGGGGGCATCGGACCTGCACCTGTCGGCCGGGCTGCCGCCGAT
GATCCGCGTGGATGGCGATGTGCGGCGGATCAACCTGCCGGCACTGGACCACAAACAGGTTCATGAACTGATCTACGACA
TCATGAACGACCGGCAACGGGTAGATTACGAAAAGTTTCTGGAAACCGACTTTTCCTTCGACGTACCGGGCGTGGCACGG
TTCCGGGTCAATGCGTTCAACCAGAATCGTGGCGCGGGGGCGGTGTTCCGGACCATTCCGTCGAAAGTCCTGACCATGGA
AGACCTGGGCATGGGCGAGGTGTTTCGCAAAGTGACCGAGGCCCCTCGCGGGCTGGTGCTGGTGACCGGGCCGACCGGTT
CCGGCAAGTCCACCACCCTGGCGGCGATGATCGATTACCTCAACACCCATAAGCACCACCACATCCTTACCATCGAGGAC
CCCATCGAGTTTGTCCACGAACCGCGCAAATGCCTGATCAACCAGCGCGAAGTGCACCGCGACACCCAGGGTTTTTCCAC
GGCCCTGCGCTCGGCCCTGCGCGAAGACCCAGACGTGATCCTGGTGGGGGAGATGCGTGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCCGAAACCGGGCACCTGGTCTTCGGCACGTTGCACACCACTTCGGCGGCCAAGACCATCGACCGGGTG
GTGGACGTGTTCCCGGGGGACGAGAAGTCCATGGTGCGCTCGATGTTGTCGGAATCATTGCAGGCGGTGATTTCCCAGAC
GCTGATCAAGAAGATCGGCGGCGGCCGGGTTGCGGCTCACGAAATCATGCTGGGCACGTCGGCGATTCGTAACCTGATTC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCGGCTCCCTGGGGATGCAGACACTGGACATGTGCCTG
AAGGATCTGGTGACCAAGGGCTTGATCAGCCGTGAACATGCGCGGGAGCGGGCGCGTACGCCGGATAGTTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

88.953

100

0.89
  pilT Pseudomonas stutzeri DSM 10701

88.372

100

0.884
  pilT Acinetobacter nosocomialis M2

80.233

100

0.802
  pilT Acinetobacter baumannii D1279779

80.233

100

0.802
  pilT Acinetobacter baumannii strain A118

80.233

100

0.802
  pilT Acinetobacter baylyi ADP1

78.779

100

0.788
  pilT Legionella pneumophila strain Lp02

71.221

100

0.712
  pilT Legionella pneumophila strain ERS1305867

71.221

100

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.087

100

0.663
  pilT Neisseria gonorrhoeae MS11

65.797

100

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.384

0.395
  pilU Vibrio cholerae strain A1552

40.597

97.384

0.395