Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LOY69_RS28320 Genome accession   NZ_CP087201
Coordinates   6353864..6354898 (-) Length   344 a.a.
NCBI ID   WP_102881165.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-059     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6348864..6359898
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY69_RS28290 (LOY69_28235) ruvX 6348997..6349434 (+) 438 WP_102862073.1 Holliday junction resolvase RuvX -
  LOY69_RS28295 (LOY69_28240) pyrR 6349551..6350057 (+) 507 WP_060841533.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  LOY69_RS28300 (LOY69_28245) - 6350083..6351087 (+) 1005 WP_011064007.1 aspartate carbamoyltransferase catalytic subunit -
  LOY69_RS28305 (LOY69_28250) - 6351084..6352355 (+) 1272 WP_265073891.1 dihydroorotase -
  LOY69_RS28310 (LOY69_28255) - 6352483..6352902 (-) 420 WP_102862075.1 NINE protein -
  LOY69_RS28315 (LOY69_28260) - 6353154..6353777 (+) 624 WP_102862076.1 C40 family peptidase -
  LOY69_RS28320 (LOY69_28265) pilT 6353864..6354898 (-) 1035 WP_102881165.1 type IV pilus twitching motility protein PilT Machinery gene
  LOY69_RS28325 (LOY69_28270) - 6355082..6355780 (+) 699 WP_170001809.1 YggS family pyridoxal phosphate-dependent enzyme -
  LOY69_RS28330 (LOY69_28275) proC 6355815..6356633 (+) 819 WP_102862079.1 pyrroline-5-carboxylate reductase -
  LOY69_RS28335 (LOY69_28280) - 6356658..6357245 (+) 588 WP_102862080.1 YggT family protein -
  LOY69_RS28340 (LOY69_28285) - 6357245..6357538 (+) 294 WP_110594334.1 DUF167 domain-containing protein -
  LOY69_RS28345 (LOY69_28290) metX 6357702..6358841 (+) 1140 WP_102862082.1 homoserine O-succinyltransferase MetX -
  LOY69_RS28350 (LOY69_28295) metW 6358849..6359469 (+) 621 WP_011064017.1 methionine biosynthesis protein MetW -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37395.18 Da        Isoelectric Point: 6.8920

>NTDB_id=628018 LOY69_RS28320 WP_102881165.1 6353864..6354898(-) (pilT) [Pseudomonas sp. B21-059]
MDITELLTLSISRGASDLHLSAGLAPMLRIDGDIRPLDSPALDAQQVLSLMHGLMSEPQRQVFASQQDLDFAYELPGVAR
FRVNAFQHSRGAAAVLRLIPSRVLSLEALGLGQVFQQIAEAPRGLVLVTGPTGSGKSTTLAAMIDHLNQHRQQHILTLED
PIEFIHPQKSCLIHQREVPRDTRSFATALRAALREDPDVILLGELRDLESIRLALTAAETGHLVFATLHTASAAKSIDRL
VDVFPGEEKALVRSMLAESLQAVIAQVLLKKVGGGRVAAHEIMLGNAAIRNLIREDKVAQMYSAIQTGGAQGMRTLDMSL
KALLGQGLISREQAREQARMPESF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=628018 LOY69_RS28320 WP_102881165.1 6353864..6354898(-) (pilT) [Pseudomonas sp. B21-059]
ATGGACATCACCGAACTCCTGACTTTGAGCATCTCCCGGGGCGCTTCCGACCTGCATCTGTCTGCCGGACTGGCGCCGAT
GCTGCGCATTGATGGCGATATTCGTCCGCTGGACAGCCCGGCCCTGGATGCACAGCAGGTGCTGTCCTTGATGCATGGCC
TGATGAGCGAGCCACAGCGGCAGGTGTTCGCCAGCCAGCAGGATCTGGACTTTGCCTACGAACTGCCGGGCGTGGCGCGG
TTCCGGGTCAATGCCTTCCAGCACTCCCGGGGAGCGGCTGCAGTGCTGCGCCTGATCCCGTCACGGGTACTGAGCTTGGA
GGCGTTGGGACTGGGGCAAGTGTTTCAGCAGATTGCCGAAGCGCCCCGGGGGCTGGTGCTGGTCACCGGGCCCACCGGTA
GCGGCAAGTCCACCACCTTGGCGGCGATGATCGATCACCTGAACCAGCATCGGCAGCAGCACATCCTCACCCTCGAAGAC
CCCATCGAGTTCATTCATCCGCAGAAAAGTTGCCTGATCCACCAGCGCGAGGTGCCGCGTGATACCCGCAGTTTCGCCAC
CGCCTTGCGTGCGGCGCTGCGGGAGGACCCGGACGTGATCCTGCTGGGAGAGTTGCGCGATCTGGAAAGCATTCGCCTGG
CCCTGACCGCCGCCGAAACCGGGCACCTGGTGTTCGCCACCCTGCACACTGCATCCGCGGCCAAGAGCATCGACCGGCTG
GTGGACGTGTTTCCGGGGGAGGAAAAGGCCCTGGTGCGCTCGATGCTGGCCGAGTCGTTGCAGGCGGTCATTGCCCAGGT
GTTGCTGAAGAAAGTGGGCGGTGGGCGGGTGGCGGCTCACGAAATCATGCTCGGCAATGCGGCGATCCGTAACCTGATTC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACCGGCGGGGCCCAGGGCATGCGTACCCTGGACATGAGCCTC
AAGGCATTGCTGGGGCAAGGGCTGATCAGCCGTGAACAGGCGCGGGAGCAGGCCCGGATGCCGGAGAGCTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

68.895

100

0.689
  pilT Pseudomonas stutzeri DSM 10701

68.895

100

0.689
  pilT Acinetobacter nosocomialis M2

66.57

100

0.666
  pilT Acinetobacter baumannii D1279779

66.57

100

0.666
  pilT Acinetobacter baumannii strain A118

66.57

100

0.666
  pilT Acinetobacter baylyi ADP1

65.988

100

0.66
  pilT Legionella pneumophila strain Lp02

62.13

98.256

0.61
  pilT Legionella pneumophila strain ERS1305867

62.13

98.256

0.61
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

61.424

97.965

0.602
  pilT Vibrio cholerae strain A1552

61.424

97.965

0.602
  pilT Neisseria meningitidis 8013

59.71

100

0.599
  pilT Neisseria gonorrhoeae MS11

59.42

100

0.596
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Vibrio cholerae strain A1552

40.299

97.384

0.392
  pilU Acinetobacter baylyi ADP1

38.841

100

0.39
  pilU Pseudomonas stutzeri DSM 10701

39.701

97.384

0.387