Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LOY36_RS26775 Genome accession   NZ_CP087193
Coordinates   5948631..5949665 (-) Length   344 a.a.
NCBI ID   WP_258671476.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-019     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5943631..5954665
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY36_RS26745 (LOY36_26705) ruvX 5943836..5944282 (+) 447 WP_018928712.1 Holliday junction resolvase RuvX -
  LOY36_RS26750 (LOY36_26710) pyrR 5944382..5944885 (+) 504 WP_018928711.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  LOY36_RS26755 (LOY36_26715) - 5944913..5945917 (+) 1005 WP_007939362.1 aspartate carbamoyltransferase catalytic subunit -
  LOY36_RS26760 (LOY36_26720) - 5945914..5947185 (+) 1272 WP_258671475.1 dihydroorotase -
  LOY36_RS26765 (LOY36_26725) - 5947223..5947654 (-) 432 WP_019580618.1 TM2 domain-containing protein -
  LOY36_RS26770 (LOY36_26730) - 5947905..5948525 (+) 621 WP_018928708.1 C40 family peptidase -
  LOY36_RS26775 (LOY36_26735) pilT 5948631..5949665 (-) 1035 WP_258671476.1 type IV pilus twitching motility protein PilT Machinery gene
  LOY36_RS26780 (LOY36_26740) - 5949723..5950409 (+) 687 WP_258671477.1 YggS family pyridoxal phosphate-dependent enzyme -
  LOY36_RS26785 (LOY36_26745) proC 5950444..5951262 (+) 819 WP_258671478.1 pyrroline-5-carboxylate reductase -
  LOY36_RS26790 (LOY36_26750) - 5951273..5951863 (+) 591 WP_019580613.1 YggT family protein -
  LOY36_RS26795 (LOY36_26755) - 5951868..5952161 (+) 294 WP_007987815.1 DUF167 domain-containing protein -
  LOY36_RS26800 (LOY36_26760) - 5952416..5953555 (+) 1140 WP_258671479.1 homoserine O-acetyltransferase -
  LOY36_RS26805 (LOY36_26765) metW 5953563..5954183 (+) 621 WP_007939351.1 methionine biosynthesis protein MetW -
  LOY36_RS26810 (LOY36_26770) - 5954210..5954644 (+) 435 WP_019650221.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38129.77 Da        Isoelectric Point: 6.8715

>NTDB_id=627802 LOY36_RS26775 WP_258671476.1 5948631..5949665(-) (pilT) [Pseudomonas sp. B21-019]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKEVHELIYDIMNDLQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMDDLGMGEVFRKITESPRGLVLVTGPTGSGKSTTLAAMIDYLNNHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRIAAHEIMLGTSAIRNLIREDKVAQMYSSIQTGGSLGMQTLDMCL
KELVTKGVISREHAREKARSPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=627802 LOY36_RS26775 WP_258671476.1 5948631..5949665(-) (pilT) [Pseudomonas sp. B21-019]
ATGGATATCACTGAGCTGCTGGCTTTCAGCGCCAAACAGGGCGCGTCCGACCTGCACCTGTCCGCCGGCCTGCCACCGAT
GATCCGTGTCGATGGCGATGTGCGACGCATCAACTTGCCGGCCCTGGACCACAAGGAGGTGCATGAGCTGATCTACGACA
TCATGAACGACCTCCAGCGGGTGGACTTCGAGAAGCATCTGGAAACCGACTTTTCCTTCGAAGTGCCCGGCGTGGCACGT
TTTCGGGTCAATGCCTTCAACCAGAACCGTGGCGCGGGCGCGGTATTCCGGACCATTCCGTCGAAGGTCCTGAGCATGGA
CGACCTGGGCATGGGGGAAGTGTTTCGCAAGATTACCGAATCCCCCCGAGGCCTGGTGCTGGTGACCGGCCCGACCGGTT
CCGGCAAGTCCACCACGCTGGCGGCGATGATCGATTACCTGAACAACCATCGCCATCACCACATCCTCACCATCGAAGAC
CCAATCGAGTTCGTCCACGAATCGCGCAAATGCCTGATCAATCAGCGTGAAGTCCATCGCGACACCCGCAGCTTTGCCAC
GGCCCTGCGTTCGGCCCTGCGTGAAGACCCGGACGTGATTCTGGTCGGGGAAATGCGTGACCTGGAGACCATTCGGCTGG
CACTGACCGCCGCCGAGACCGGCCACCTGGTGTTCGGCACGCTGCACACCACATCGGCGGCAAAAACCATCGACAGGGTG
GTGGACGTGTTTCCGGGCGACGAGAAATCGATGGTGCGTTCGATGCTCTCCGAGTCGTTGCTGGCGGTGGTGTCTCAGAC
TTTGATCAAGAAAATCGGCGGCGGGCGGATTGCCGCCCACGAAATCATGCTCGGCACCTCGGCGATCCGTAACCTGATCC
GTGAAGACAAGGTGGCGCAGATGTACTCGTCGATTCAGACCGGAGGGTCGCTGGGGATGCAGACGCTGGATATGTGCCTG
AAGGAGCTGGTGACCAAAGGCGTGATCAGTCGCGAGCATGCGCGGGAGAAGGCGCGCTCGCCGGATAATTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

87.5

100

0.875
  pilT Pseudomonas aeruginosa PAK

86.919

100

0.869
  pilT Acinetobacter nosocomialis M2

79.07

100

0.791
  pilT Acinetobacter baumannii D1279779

79.07

100

0.791
  pilT Acinetobacter baumannii strain A118

79.07

100

0.791
  pilT Acinetobacter baylyi ADP1

78.198

100

0.782
  pilT Legionella pneumophila strain Lp02

71.802

100

0.718
  pilT Legionella pneumophila strain ERS1305867

71.802

100

0.718
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.509
  pilU Acinetobacter baylyi ADP1

41.04

100

0.413
  pilU Pseudomonas stutzeri DSM 10701

40.29

100

0.404
  pilU Vibrio cholerae strain A1552

39.71

100

0.398