Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LOY55_RS28715 Genome accession   NZ_CP087176
Coordinates   6263006..6264040 (-) Length   344 a.a.
NCBI ID   WP_046029740.1    Uniprot ID   A0A2V2ALY2
Organism   Pseudomonas sp. B21-040     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6258006..6269040
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY55_RS28685 (LOY55_28645) ruvX 6258222..6258659 (+) 438 WP_003195067.1 Holliday junction resolvase RuvX -
  LOY55_RS28690 (LOY55_28650) pyrR 6258748..6259251 (+) 504 WP_046029746.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  LOY55_RS28695 (LOY55_28655) - 6259278..6260282 (+) 1005 WP_046029744.1 aspartate carbamoyltransferase catalytic subunit -
  LOY55_RS28700 (LOY55_28660) - 6260279..6261550 (+) 1272 WP_109785625.1 dihydroorotase -
  LOY55_RS28705 (LOY55_28665) - 6261638..6262069 (-) 432 WP_046029742.1 TM2 domain-containing protein -
  LOY55_RS28710 (LOY55_28670) - 6262320..6262940 (+) 621 WP_046029741.1 C40 family peptidase -
  LOY55_RS28715 (LOY55_28675) pilT 6263006..6264040 (-) 1035 WP_046029740.1 type IV pilus twitching motility protein PilT Machinery gene
  LOY55_RS28720 (LOY55_28680) - 6264098..6264784 (+) 687 WP_046029739.1 YggS family pyridoxal phosphate-dependent enzyme -
  LOY55_RS28725 (LOY55_28685) proC 6264821..6265639 (+) 819 WP_077431104.1 pyrroline-5-carboxylate reductase -
  LOY55_RS28730 (LOY55_28690) - 6265664..6266251 (+) 588 WP_046029737.1 YggT family protein -
  LOY55_RS28735 (LOY55_28695) - 6266251..6266541 (+) 291 WP_109785627.1 DUF167 domain-containing protein -
  LOY55_RS28740 (LOY55_28700) - 6266720..6267859 (+) 1140 WP_223522931.1 homoserine O-acetyltransferase -
  LOY55_RS28745 (LOY55_28705) metW 6267867..6268487 (+) 621 WP_046029734.1 methionine biosynthesis protein MetW -
  LOY55_RS28750 (LOY55_28710) - 6268514..6268948 (+) 435 WP_109785629.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38010.61 Da        Isoelectric Point: 7.0158

>NTDB_id=627368 LOY55_RS28715 WP_046029740.1 6263006..6264040(-) (pilT) [Pseudomonas sp. B21-040]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVQELIHDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMDDLGMGEVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNSHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLVKKIGGGRIAAHEIMLGTSAIRNLIREDKVAQMYSSIQTGGSLGMQTLDMCL
KELVTKGLISREHARDKARSPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=627368 LOY55_RS28715 WP_046029740.1 6263006..6264040(-) (pilT) [Pseudomonas sp. B21-040]
ATGGATATCACTGAGCTGCTGGCTTTCAGCGCTAAACAGGGCGCATCCGACCTGCACTTGTCAGCTGGCCTGCCCCCGAT
GATCCGTGTCGACGGCGATGTACGGCGCATCAACCTGCCGGCCCTGGATCACAAACAGGTACAGGAACTGATCCACGACA
TCATGAACGACACCCAGCGGGTGGACTTCGAGAAACACCTGGAAACCGATTTCTCATTCGAAGTGCCTGGTGTGGCGCGT
TTTCGGGTGAACGCGTTTAACCAGAACCGTGGCGCCGGGGCGGTGTTCAGGACCATTCCATCGAAGGTTCTGAGCATGGA
CGACCTCGGCATGGGCGAGGTGTTTCGCAAGATCACCGATGCGCCGCGCGGGCTGGTGCTGGTCACCGGGCCGACCGGTT
CCGGCAAGTCCACCACGTTGGCGGCGATGATCGACTACCTGAACAGCCATCGCCATCACCACATCCTCACCATCGAAGAC
CCGATCGAGTTTGTCCACGAATCGCGCAAATGCCTGATCAACCAGCGCGAGGTGCATCGCGATACGCGCAGCTTTGCCAC
GGCCCTGCGCTCGGCCCTGCGCGAAGACCCGGACGTGATTCTGGTAGGGGAGATGCGGGACCTGGAGACCATTCGCCTGG
CGCTGACCGCTGCCGAGACCGGGCATCTGGTGTTTGGCACGCTGCACACCACGTCAGCGGCGAAAACCATCGACCGGGTG
GTGGATGTGTTTCCGGGGGATGAGAAGTCGATGGTGCGGTCGATGCTGTCGGAATCGTTGCTGGCGGTGGTGTCGCAGAC
ACTGGTGAAGAAGATCGGCGGCGGGCGGATTGCGGCGCACGAGATCATGTTGGGTACGTCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCATCGATTCAGACGGGAGGATCGCTGGGGATGCAGACGCTGGATATGTGCTTG
AAGGAGTTGGTGACCAAGGGCTTGATCAGTCGCGAGCATGCGCGGGATAAGGCGCGGTCGCCGGATAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2V2ALY2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

87.209

100

0.872
  pilT Pseudomonas aeruginosa PAK

86.337

100

0.863
  pilT Acinetobacter nosocomialis M2

78.488

100

0.785
  pilT Acinetobacter baumannii D1279779

78.488

100

0.785
  pilT Acinetobacter baumannii strain A118

78.488

100

0.785
  pilT Acinetobacter baylyi ADP1

77.326

100

0.773
  pilT Legionella pneumophila strain Lp02

71.512

100

0.715
  pilT Legionella pneumophila strain ERS1305867

71.512

100

0.715
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.953

97.965

0.666
  pilT Vibrio cholerae strain A1552

67.953

97.965

0.666
  pilT Neisseria meningitidis 8013

65.797

100

0.66
  pilT Neisseria gonorrhoeae MS11

65.507

100

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.509
  pilU Pseudomonas stutzeri DSM 10701

40.87

100

0.41
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407
  pilU Vibrio cholerae strain A1552

40.29

100

0.404