Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LOY43_RS26735 Genome accession   NZ_CP087175
Coordinates   5991770..5992804 (-) Length   344 a.a.
NCBI ID   WP_095049465.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-041     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5986770..5997804
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY43_RS26705 (LOY43_26670) ruvX 5986999..5987436 (+) 438 WP_064589634.1 Holliday junction resolvase RuvX -
  LOY43_RS26710 (LOY43_26675) pyrR 5987517..5988023 (+) 507 WP_034153653.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  LOY43_RS26715 (LOY43_26680) - 5988051..5989055 (+) 1005 WP_007913665.1 aspartate carbamoyltransferase catalytic subunit -
  LOY43_RS26720 (LOY43_26685) - 5989052..5990323 (+) 1272 WP_095125844.1 dihydroorotase -
  LOY43_RS26725 (LOY43_26690) - 5990426..5990857 (-) 432 WP_007913651.1 TM2 domain-containing protein -
  LOY43_RS26730 (LOY43_26695) - 5991106..5991723 (+) 618 WP_095049466.1 C40 family peptidase -
  LOY43_RS26735 (LOY43_26700) pilT 5991770..5992804 (-) 1035 WP_095049465.1 type IV pilus twitching motility protein PilT Machinery gene
  LOY43_RS26740 (LOY43_26705) - 5992861..5993547 (+) 687 WP_095125843.1 YggS family pyridoxal phosphate-dependent enzyme -
  LOY43_RS26745 (LOY43_26710) proC 5993571..5994389 (+) 819 WP_095125842.1 pyrroline-5-carboxylate reductase -
  LOY43_RS26750 (LOY43_26715) - 5994400..5994990 (+) 591 WP_095049462.1 YggT family protein -
  LOY43_RS26755 (LOY43_26720) - 5994987..5995274 (+) 288 WP_095049461.1 DUF167 domain-containing protein -
  LOY43_RS26760 (LOY43_26725) - 5995454..5996592 (+) 1139 Protein_5269 homoserine O-acetyltransferase -
  LOY43_RS26765 (LOY43_26730) metW 5996600..5997220 (+) 621 WP_258708938.1 methionine biosynthesis protein MetW -
  LOY43_RS26770 (LOY43_26735) - 5997247..5997681 (+) 435 WP_095049459.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38085.76 Da        Isoelectric Point: 7.0157

>NTDB_id=627340 LOY43_RS26735 WP_095049465.1 5991770..5992804(-) (pilT) [Pseudomonas sp. B21-041]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLAMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLLTKGLISREHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=627340 LOY43_RS26735 WP_095049465.1 5991770..5992804(-) (pilT) [Pseudomonas sp. B21-041]
ATGGATATCACTGAACTGCTGGCCTTCAGCGCCAAACAAGGCGCTTCCGACCTGCACCTGTCGGCCGGTCTGCCGCCGAT
GATCCGTGTCGATGGCGATGTGCGGCGGATCAATCTGCCGGCGCTGGATCACAAGCAAGTGCACGAACTGATCTACGACA
TCATGAACGACACCCAGCGGGTCGACTTCGAGAAACATCTGGAAACCGACTTTTCCTTCGAAGTGCCGGGTGTGGCGCGT
TTCCGCGTCAACGCTTTCAACCAGAACCGTGGCGCAGGCGCCGTATTCCGTACCATTCCGTCGAAAGTGCTGAGCATGGA
AGACCTCGCCATGGGCGACGTCTTCCGCAAGATTACCGATGCGCCGCGCGGTCTGGTGCTGGTCACCGGGCCGACCGGTT
CCGGCAAATCAACCACGCTGGCGGCGATGATCGATTACCTCAATACCCACCGGCATCACCACATCCTGACTATCGAAGAT
CCGATCGAGTTTGTTCACGAATCGCGCAAATGCCTGATCAATCAGCGTGAAGTCCACCGTGATACCCGCAGTTTCGCCAC
TGCGTTGCGCTCGGCACTGCGTGAAGACCCGGACGTGATTCTGGTCGGCGAGATGCGTGACCTGGAAACCATTCGCCTGG
CGCTGACCGCTGCCGAAACCGGGCACTTGGTATTCGGCACGCTGCACACCACCTCGGCGGCGAAAACCATCGACCGCGTG
GTCGACGTGTTCCCCGGTGACGAGAAGTCGATGGTGCGTTCGATGCTGTCCGAGTCGTTGCTGGCGGTGGTCTCGCAGAC
GTTGATCAAGAAGATCGGCGGCGGGCGGGTGGCGGCGCACGAGATCATGCTGGGCACGTCGGCGATCCGTAACCTGATCC
GTGAGGACAAGGTGGCGCAGATGTATTCGGCGATTCAGACGGGTGGGTCGTTGGGCATGCAGACACTGGACATGTGCCTG
AAGGATCTGCTGACCAAGGGCTTGATCAGCCGCGAGCATGCGCGGGAGAAGGCGCGGACGCCGGATAATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.081

100

0.881
  pilT Pseudomonas aeruginosa PAK

87.5

100

0.875
  pilT Acinetobacter nosocomialis M2

79.36

100

0.794
  pilT Acinetobacter baumannii D1279779

79.36

100

0.794
  pilT Acinetobacter baumannii strain A118

79.36

100

0.794
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Legionella pneumophila strain Lp02

72.189

98.256

0.709
  pilT Legionella pneumophila strain ERS1305867

72.189

98.256

0.709
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.087

100

0.663
  pilT Neisseria gonorrhoeae MS11

65.797

100

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

39.42

100

0.395