Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M5T33_RS19990 Genome accession   NZ_CP103739
Coordinates   4061833..4062813 (-) Length   326 a.a.
NCBI ID   WP_001309723.1    Uniprot ID   -
Organism   Escherichia coli strain 3359     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4056833..4067813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5T33_RS19960 (M5T33_19960) yggI 4057557..4058054 (+) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -
  M5T33_RS19965 (M5T33_19965) endA 4058149..4058856 (+) 708 WP_000286500.1 deoxyribonuclease I -
  M5T33_RS19970 (M5T33_19970) rsmE 4058936..4059667 (+) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M5T33_RS19975 (M5T33_19975) gshB 4059680..4060630 (+) 951 WP_000593273.1 glutathione synthase -
  M5T33_RS19980 (M5T33_19980) yqgE 4060739..4061302 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  M5T33_RS19985 (M5T33_19985) ruvX 4061302..4061718 (+) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  M5T33_RS19990 (M5T33_19990) pilT 4061833..4062813 (-) 981 WP_001309723.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M5T33_RS19995 (M5T33_19995) yggS 4062831..4063535 (+) 705 WP_000997806.1 pyridoxal phosphate homeostasis protein -
  M5T33_RS20000 (M5T33_20000) yggT 4063553..4064119 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  M5T33_RS20005 (M5T33_20005) yggU 4064116..4064406 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  M5T33_RS20010 (M5T33_20010) rdgB 4064414..4065007 (+) 594 WP_001174736.1 XTP/dITP diphosphatase -
  M5T33_RS20015 (M5T33_20015) hemW 4065000..4066136 (+) 1137 WP_000239946.1 radical SAM family heme chaperone HemW -
  M5T33_RS20020 (M5T33_20020) - 4066449..4067435 (+) 987 WP_000784004.1 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35940.15 Da        Isoelectric Point: 5.7980

>NTDB_id=624650 M5T33_RS19990 WP_001309723.1 4061833..4062813(-) (pilT) [Escherichia coli strain 3359]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGVILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINIPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=624650 M5T33_RS19990 WP_001309723.1 4061833..4062813(-) (pilT) [Escherichia coli strain 3359]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGCGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCGTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTCGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCTGCGTTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGGAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACATTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTAGGGGAAGGACGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain Lp02

44.648

100

0.448
  pilT Legionella pneumophila strain ERS1305867

44.648

100

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

39.21

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

36.97

100

0.374
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362