Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M5R25_RS04595 Genome accession   NZ_CP103657
Coordinates   960639..961619 (+) Length   326 a.a.
NCBI ID   WP_001361345.1    Uniprot ID   -
Organism   Escherichia coli strain 3150     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 955639..966619
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5R25_RS04565 (M5R25_04565) - 956017..957003 (-) 987 WP_001564022.1 TRAP transporter substrate-binding protein -
  M5R25_RS04570 (M5R25_04570) hemW 957316..958452 (-) 1137 WP_000239986.1 radical SAM family heme chaperone HemW -
  M5R25_RS04575 (M5R25_04575) rdgB 958445..959038 (-) 594 WP_001174738.1 XTP/dITP diphosphatase -
  M5R25_RS04580 (M5R25_04580) yggU 959046..959336 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  M5R25_RS04585 (M5R25_04585) yggT 959333..959899 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  M5R25_RS04590 (M5R25_04590) yggS 959917..960621 (-) 705 WP_000997793.1 pyridoxal phosphate homeostasis protein -
  M5R25_RS04595 (M5R25_04595) pilT 960639..961619 (+) 981 WP_001361345.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M5R25_RS04600 (M5R25_04600) ruvX 961832..962248 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  M5R25_RS04605 (M5R25_04605) yqgE 962248..962811 (-) 564 WP_001339287.1 YqgE/AlgH family protein -
  M5R25_RS04610 (M5R25_04610) gshB 962920..963870 (-) 951 WP_000593278.1 glutathione synthase -
  M5R25_RS04615 (M5R25_04615) rsmE 963883..964614 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M5R25_RS04620 (M5R25_04620) endA 964694..965401 (-) 708 WP_000286500.1 deoxyribonuclease I -
  M5R25_RS04625 (M5R25_04625) yggI 965496..965993 (-) 498 WP_023908971.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35970.18 Da        Isoelectric Point: 5.7980

>NTDB_id=624031 M5R25_RS04595 WP_001361345.1 960639..961619(+) (pilT) [Escherichia coli strain 3150]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAVPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=624031 M5R25_RS04595 WP_001361345.1 960639..961619(+) (pilT) [Escherichia coli strain 3150]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGCGAATGGAAGCTGTGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
TGCCGCATTGCGGGAAGATCCCGATGTGATTTTGCTCGGAGAGCTACGTGACAGCGAGACAATCCGTCTGGCGCTTACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCGGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGACGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

37.267

98.773

0.368