Detailed information NTG0623779

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: M5S78_RS03955
• Genome accession: NZ_CP103626
• Coordinates: 812983..813963 (+)
• Length: 326 a.a.
• NCBI ID: WP_001324408.1
• Uniprot ID: -
• Organism: Escherichia coli strain 4012
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 807983..818963

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M5S78_RS03925 (M5S78_03925)	yggM	808498..809505 (+)	1008	WP_000745217.1	DUF1202 family protein	-
M5S78_RS03930 (M5S78_03930)	hemW	809660..810796 (-)	1137	WP_000239928.1	radical SAM family heme chaperone HemW	-
M5S78_RS03935 (M5S78_03935)	rdgB	810789..811382 (-)	594	WP_001174743.1	XTP/dITP diphosphatase	-
M5S78_RS03940 (M5S78_03940)	yggU	811390..811680 (-)	291	WP_001277222.1	DUF167 family protein YggU	-
M5S78_RS03945 (M5S78_03945)	yggT	811677..812243 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
M5S78_RS03950 (M5S78_03950)	yggS	812261..812965 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
M5S78_RS03955 (M5S78_03955)	pilT	812983..813963 (+)	981	WP_001324408.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
M5S78_RS03960 (M5S78_03960)	ruvX	814126..814542 (-)	417	WP_000017106.1	Holliday junction resolvase RuvX	-
M5S78_RS03965 (M5S78_03965)	yqgE	814542..815105 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
M5S78_RS03970 (M5S78_03970)	gshB	815214..816164 (-)	951	WP_000593273.1	glutathione synthase	-
M5S78_RS03975 (M5S78_03975)	rsmE	816177..816908 (-)	732	WP_001222508.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
M5S78_RS03980 (M5S78_03980)	endA	816988..817695 (-)	708	WP_000286500.1	deoxyribonuclease I	-
M5S78_RS03985 (M5S78_03985)	yggI	817790..818287 (-)	498	WP_000858396.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35873.07 Da
• Isoelectric Point: 5.5857

>NTDB_id=623779 M5S78_RS03955 WP_001324408.1 812983..813963(+) (pilT) [Escherichia coli strain 4012]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGCVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=623779 M5S78_RS03955 WP_001324408.1 812983..813963(+) (pilT) [Escherichia coli strain 4012]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGTGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACTTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTAACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGATGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTTTCAGCAGCGA
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.235	100	0.494
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.235	100	0.494
pilT	Neisseria meningitidis 8013	48.476	100	0.488
pilT	Neisseria gonorrhoeae MS11	48.171	100	0.485
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter baumannii D1279779	46.177	100	0.463
pilT	Acinetobacter nosocomialis M2	46.177	100	0.463
pilT	Acinetobacter baumannii strain A118	46.177	100	0.463
pilT	Pseudomonas stutzeri DSM 10701	46.177	100	0.463
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain ERS1305867	44.954	100	0.451
pilT	Legionella pneumophila strain Lp02	44.954	100	0.451
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.824	97.546	0.408
pilU	Vibrio cholerae strain A1552	39.21	100	0.396
pilU	Pseudomonas stutzeri DSM 10701	37.273	100	0.377
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362